Vitamin K 3 (VK 3 ), a synthetic blood coagulation vitamin with fat-solubility, easily results in side-effect in animal body due to its toxicity and improper addition of animal feedstuffs. In this study, a novel sensor based on glassy carbon electrode (GCE) modified with a conducting polymer-both nano-noble metals and two-dimensional nano-carbon composite consisting of poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), carboxymethyl cellulose (CMC) and reduced graphene oxide@palladium (rGO@Pd) was successfully used for the voltammetric detection of VK 3 in animal blood and feedstuff samples. Under the optimized conditions, PEDOT:PSS-CMC-rGO@Pd/GCE/GCE displayed the excellent synergistically electrocatalytic ability for the electrochemical oxidation-reduction of VK 3 , which exhibited a good voltammetric response for VK 3 in the concentration range from 4 × 10 -7 to 9 × 10 -5 M, with a limit of detection of 1.4 × 10 -8 M. Furthermore, a facile preparation, high sensitivity, good selectivity, superior sensing stability, and satisfactory practicability of PEDOT:PSS-CMC-rGO@Pd/GCE/GCE for sensing VK 3 suggested that the potential applicability of PEDOT:PSS-CMC-rGO@Pd provided a promising electrochemical sensing platform for the determination of VK 3 in pharmaceutical and feed inspection.