Green Synthesis of Gold and Silver Nanoparticles by Using Amorphophallus paeoniifolius Tuber Extract and Evaluation of Their Antibacterial Activity

Abstract

In this report, we discussed rapid, facile one-pot green synthesis of gold and silver nanoparticles (AuNPs and AgNPs) by using tuber extract of Amorphophallus paeoniifolius, and evaluated their antibacterial activity. AuNPs and AgNPs were synthesized by mixing their respective precursors (AgNO3 and HAuCl4) with tuber extract of Amorphophallus paeoniifolius as the bio-reducing agent. Characterization of AuNPs and AgNPs were confirmed by applying UV-vis spectroscopy, field-emission scanning electron microscopy (FESEM), X-ray diffraction (XRD) analysis, Fourier transform infrared spectroscopy (FTIR), and energy dispersive X-ray spectroscopy (EDS). From UV-vis characterization, surface plasmon resonance spectra were found at 530 nm for AuNPs and 446 nm for AgNPs. XRD data confirmed that both synthesized nanoparticles were face-centered cubic in crystalline nature, and the average crystallite sizes for the assign peaks were 13.3 nm for AuNPs and 22.48 nm for AgNPs. FTIR data evaluated the characteristic peaks of different phytochemical components of tuber extract, which acted as the reducing agent, and possibly as stabilizing agents. The antibacterial activity of synthesized AuNPs and AgNPs were examined in Muller Hinton agar, against two Gram-positive and four Gram-negative bacteria through the disc diffusion method. AuNPs did not show any inhibitory effect, while AgNPs showed good inhibitory effect against both Gram-positive and Gram-negative bacteria.

Ligustrum lucidum Leaf Extract-Assisted Green Synthesis of Silver Nanoparticles and Nano-Adsorbents Having Potential in Ultrasound-Assisted Adsorptive Removal of Methylene Blue Dye from Wastewater and Antimicrobial Activity

“…The findings of the antibacterial assay revealed that there was a clear increase in the activities of AgNPs against S. aureus . These findings were analogous to the previous reports in the literature that silver nanoparticles had strong antibacterial activities [ 55 , 76 , 77 ]. Composite was also active for S. aureus , while unaccompanied nanoparticles of L. lucidum were more significantly active.…”

Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds

“…Thus far, utilization of leaf and fruit extracts of various plants are the most commonly reported. Among many plants, sweet potato is an interesting candidate for this purpose, not only because it contains high concentration of various reducing agents (e.g., reducing sugars, phenolic compounds, vitamin C, β-carotene), but also because it has a lot of unexplored regional variants, each offering slightly different compositions to one another [ 39 , 40 , 41 , 42 ].…”

Characterization And Cytotoxicity of Novel Synthesis Selenium Nanoparticles Stabilized By Vitamin E TPGS

“…The XRD analysis confirming that the produced SeNPs possessed a hexagonal phase structure (more stable form at room temperature), It's worth noting that the broadening of the XRD peaks indicates that the grain sizes of the produced powder are on the nano scale. The Scherer formula equation applied to calculate the average crystalline size for all assigned peaks of the synthesized SeNPs was obtained equal to 5.08 nm [34]. The contrasting of this result appear in XRD spectrum of the SeNPs prepared by bio-reduction of sodium selenate (Na 2 SeO 4 ) exhibited a broad peak at a 2theta angle of 15-35 degree indicating amorphous nature of selenium crystal this return stabilizer effect [35].…”