The aim of this research was to investigate synergistic effect of combination two related antioxidant agents by novel synthesis of selenium nanoparticles functionalized with D-α- Tocopherol polyethylene glycol 1000 succinate (TPGS) as a capping agent during chemical reduction of sodium selenite by ascorbic acid. Particle size, morphology, crystallite size of synthesized Se-TPGS were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Field Emission Scanning Electron Microscopy (FESEM), Ultraviolet-Visible spectrophotometry (UV/Vis), X-Ray diffraction (XRD). The in vitro antioxidant activity, cell toxicity and blood compatibility assays were investigate respectively. The DLS results revealed that the Se-TPGS possess very low polydispersity index value (PDI = 0.03) and having hydrodynamic particle size of 83.33 ± 14.7 nm with negatively value for zeta potential equal to -19.5 mV. Both SEM and TEM show regular spherical shape of SeNPs with size measurement less than DLS measuring. XRD result confirming that the produced SeNPs possessed a standard crystalline hexagonal phase structure. DPPH assay of total antioxidant activity display high antioxidant activity of Se-TPGS NPs when comparing with ascorbic acid as a reference material. The cytotoxicity of new formula Se-TPGS investigated for both cancerous (MCF-7) and normal (HBL-100) breast cell lines with significantly different. This study provides a facile and green method for chemically synthesizing stable SeNPs which are suitable for further evaluation in therapeutic applications. The SeNPs could be a blueprint in the field of biomedical application as an alternative chemotherapy for fighting cancerous cells.
As cancer-related deaths continue to rise, developments in nanotechnology have emerged as a feasible option for finding successful treatments targeting cancerous cells while avoiding all of the drawbacks of traditional drugs. Selenium nanoparticles (SeNPs) have been reported to exhibit an inhibitory effect on cancerous cells. The aim of the present study was to use the drug delivery systems poly(lactic-coglycolic acid) (PLGA) and poly(lactic-co-glycolic acid-poly(ethylene glycol)-folic acid (PLGA-PEG-FA) to encapsulate SeNPs and investigate their antineoplastic effects against two cell line types (MCF-7 as a positive folate receptor and HBL as a negative folate receptor) by exploiting overexpression features in some types of cancer cells to ensure delivery of drug molecules at high dosages toward targeted cells and circumvent normal cells/tissues. SeNPs were chemically synthesized and characterized with dynamic light scattering (DLS) and transmission electronic microscopy (TEM). The cytotoxicity of both nanomaterials was evaluated against MCF-7 and HBL cells by using the methyl thiazolyl tetrazolium (MTT) assay which showed a high cytotoxic effect against MCF-7 cells with a lesser effect against HBL cells. Additionally, an apoptosis assay was also performed by using acridine orange/ethidium bromide dual staining, and the antioxidant effect was also investigated by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) colorimetric method with high antioxidant potential for both formulations. They showed nonhemolytic activity on human red blood cells. This work could be considered promising for pharmaceutical formulation.
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