Green synthesis of biocompatible carboxylic curdlan-capped gold nanoparticles and its interaction with protein

Yan, Jing; Liu, Jinlin; Sun, Yujia; Tang, Shuang; Mo, Zheng-ying; Liu, Yuanshuai

doi:10.1016/j.carbpol.2014.10.048

Cited by 32 publications

(11 citation statements)

References 39 publications

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“…67% structure of BSA comprises of α‐helix. Hence, changes in α‐helical structure could express high intensity in the far UV region 32 . Interaction of 3‐carboxyphenoxathiin with BSA could decrease intensity up to 2.7% in the α‐helical content of BSA 30 .…”

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxicity, macromolecular interaction and antioxidant potential of dual coated selenium nanoparticles

Shinde

Desai²

2022

J Biomed Mater Res

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The present research focuses on synthesizing selenium nanoparticles (Se NPs) coated with Methionine and Folic acid. selenium‐methionine‐folate nanoparticles (Se‐Met‐Fa NPs) were prepared by the chemical precipitation method. These were characterized by UV–Vis spectroscopy, FTIR, X‐ray diffraction, Zeta potential, ICP‐AES, TEM, and Raman spectroscopy. The average diameter of nanoparticles was determined by TEM was 50 nm. In Vitro viability of cells exposed to Se‐Met‐Fa NPs were studied using MTT and AO/EB assay. Approximately 80% of cells were viable at 100 μg/ml concentration after 24 h of incubation, suggestive of the safety of nanoparticles. Macromolecular interaction studies were carried out with plasmid DNA and Bovine serum albumin (BSA) protein. UV–Vis Spectroscopy showed ground state complex formation of Se‐Met‐Fa NPs with BSA. Intrinsic fluorescence of BSA was quenched by Se‐met‐Fa NPs via Static quenching and was observed under Spectro‐fluorimetry. Conformational structural change in α‐Helices of BSA was observed to be 4.4% after the interaction with Se‐Met‐Fa NPs, and it was studied using CD spectroscopy. At 250 μg/ml Se‐Met‐Fa NPs prevented oxidative damage of Plasmid DNA. The total antioxidant property of Se‐Met‐Fa NPs expressed in terms of scavenging of free DPPH radicals. Ten micrograms per milliliter could inhibit 41% of DPPH, proving its scavenger role at the lowest concentration. Nanoparticles comprising antioxidant semi‐conducting cores and encapsulated by biomaterials that are highly bioavailable can be promising therapeutic agents for inflammation and oxidative stress disorders studies.

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Section: Discussionmentioning

confidence: 99%

In vitro cytotoxicity, macromolecular interaction and antioxidant potential of dual coated selenium nanoparticles

Shinde

Desai²

2022

J Biomed Mater Res

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“…For curdlan, the carboxylic groups of curdlan derivatives such as carboxylic curdlan and carboxymethyl curdlan have the ability to adsorb metal ions by electrostatic attractive forces. These functional groups reduce the metal ions, and the necessary nucleation is created to form stabilized clusters during synthesis of Au-NPs, Ag-NPs, and ZnO-NP, as well as stabilization of Se-NPs [57,60,73,74]. In this context, it is noteworthy to mention that curdlan is not soluble in water; therefore, it is carboxymethylated, oxidized, and its sodium salt is formed, in order to improve their properties [110].…”

Section: Potential Mechanisms That Explain the Synthesis Of Metal Nanmentioning

confidence: 99%

“…In this context, other biomedical measurements related with other applications are cysteine detection by absorption spectroscopy and visual inspection during color change [71], biocompatibility assays by cytotoxicity studies using MTT method [54,95,96], toxicological tests by measuring in vivo studies of oral, dermal, and immunotoxicity, as well as genotoxicity, carcinogenicity, biochemical and hematological parameters, and histopathological analysis [96], interaction with protein assay employing fluorescence, circular dichroism spectroscopies and UV-visible spectroscopy [60,74], in vitro ROS scavenging assay and antioxidant activities by DPPH (2,2-diphenyl-1-picrylhydrazyl), Trolox and ferric-reducing ability of plasma (FRAP) tests [54,57], and in vitro MRI of cell phantoms to assess nanoprobes for cell labeling by MRI [56]. Thus, there are many ways to measure the potential of metal nanoparticles synthesized by EPSs as a reducing and/or stabilizing agent in different important biomedical applications today.…”

Section: Main Techniques Used To Characterize Metalmentioning

confidence: 99%

“…Curdlan and other modified compounds of EPSs have been useful to produce Ag-NPs using 4-acetamido-2,2,6,6tetramethypiperidine-1-oxyl radical-(TEMPO-) oxidized curdlan [73], and also, nontoxic and biocompatible Au-NPs were produced using carboxylic curdlan. For the last study, the interaction of Au-NPs with bovine serum albumin were tested, indicating that these nanoparticles do not produce significant changes in protein structure [74].…”

mentioning

confidence: 99%

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Bacterial Exopolysaccharides as Reducing and/or Stabilizing Agents during Synthesis of Metal Nanoparticles with Biomedical Applications

Escárcega‐González

Garza-Cervantes

Vázquez-Rodríguez

et al. 2018

International Journal of Polymer Science

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Bacterial exopolysaccharides (EPSs) are biomolecules secreted in the extracellular space and have diverse biological functionalities, such as environmental protection, surface adherence, and cellular interactions. EPSs have been found to be biocompatible and eco-friendly, therefore making them suitable for applications in many areas of study and various industrial products. Recently, synthesis and stabilization of metal nanoparticles have been of interest because their usefulness for many biomedical applications, such as antimicrobials, anticancer drugs, antioxidants, drug delivery systems, chemical sensors, contrast agents, and as catalysts. In this context, bacterial EPSs have been explored as agents to aid in a greener production of a myriad of metal nanoparticles, since they have the ability to reduce metal ions to form nanoparticles and stabilize them acting as capping agents. In addition, by incorporating EPS to the metal nanoparticles, the EPS confers them biocompatibility. Thus, the present review describes the main bacterial EPS utilized in the synthesis and stabilization of metal nanoparticles, the mechanisms involved in this process, and the different applications of these nanoparticles, emphasizing in their biomedical applications.

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“…The naturally occurring EPSs are abundant, readily available, biodegradable, and potentially non-toxic ( Moscovici, 2015 ; Bhatia, 2016 ). EPSs have found a vast range of applications in nanotechnology, like green synthesis of metal nanoparticles ( Kanmani and Lim, 2013 ; Sathiyanarayanan et al, 2017 ), hydrogel ( Na et al, 2000 ; Maiti et al, 2011 ; Koop et al, 2015 ), coating materials ( Jang et al, 2013 ; You et al, 2014 ; Bondarenko et al, 2016 ), stabilizers ( Dey et al, 2016 ), nanoparticles ( Wang et al, 2013 ; Guo et al, 2014 ; Zhang et al, 2016 ), capping agents ( Wu et al, 2012 ; Yan et al, 2015 ). Even more, EPS can self-assembled into different nanostructures like nanofibers ( Xu et al, 2013 ; Chen et al, 2016 ) and nanoparticles ( Li et al, 2017 ), which could be employed as promising bio-active carriers for DNA ( Liu et al, 2014 , 2015 ), protein ( Takahashi et al, 2014 ), and drug ( Takedatsu et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharide-Derived Carbon Dots for Microbial Viability Assessment

Lin

Chen

2018

Front. Microbiol.

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Fluorescent dye staining combined with fluorescence microscopy or flow cytometry is becoming a routine way to monitor microorganism viability that is necessary for food safety, antibiotic development, and human health. However, the conventional live/dead assay dyes suffer from high cost, inconvenient staining steps, and high cytotoxicity, which is urgently needed to overcome. Herein, cheap carbon dots, CDs-EPS605, were reported to successfully assess microbial viability in a convenient way with neglectable cytotoxicity. The fluorescent N-doped CDs-EPS605 could be facilely prepared from bacterial amino exopolysaccharide (EPS) by one-step hydrothermal carbonization, which is cost-effective and sustainable. The negatively charged CDs-EPS605 consisted of C, H, O, N, P, and S, and featured various functional groups, including -COOH, -OH, -CONH-, and -NH2. CDs-EPS605 were observed to sensitively and selectively stain dead microorganisms instead of live ones to enable discrimination of live/dead microorganisms. The labeling method with CDs-EPS605 did not require protection from light, or washing, which is convenient. Additionally, CDs-EPS605 displayed better photostability and much less cytotoxicity compared to the commercial counterpart. Altogether, CDs-EPS605 represent a simple, yet powerful staining agent for microbial viability assessment, and at the same time enrich the current applications of microbial EPS.

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Green synthesis of biocompatible carboxylic curdlan-capped gold nanoparticles and its interaction with protein

Cited by 32 publications

References 39 publications

In vitro cytotoxicity, macromolecular interaction and antioxidant potential of dual coated selenium nanoparticles

In vitro cytotoxicity, macromolecular interaction and antioxidant potential of dual coated selenium nanoparticles

Bacterial Exopolysaccharides as Reducing and/or Stabilizing Agents during Synthesis of Metal Nanoparticles with Biomedical Applications

Exopolysaccharide-Derived Carbon Dots for Microbial Viability Assessment

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