Green Synthesis of Bioactive CuO@Fe3O4@Walnut Shell Nanocomposite Using Crataegus azarolus Var. aronia L. Extract and its Antivasoconstrictive Action on Rat’s Aortic Smooth Muscle

Abstract

The antioxidant potential of Crataegus azarolus Var. aronia aqueous extract was used as reducing and stabilizing media to the green synthesis of bioactive CuO@Fe3O4@Walnut shell nanocomposite (NCs). The ability of the plant extract as bioreducing medium was proved using Fourier-transform infrared spectroscopy and ultraviolet–visible spectroscopy. While, the structural identification of synthesized CuO@Fe3O4@Walnut shell NCs was proved using the scanning electron microscopy, electron dispersive spectroscopy, elemental mapping, and point analysis. Then, the antivasoconstriction effects of CuO@Fe3O4@Walnut shell NCs (5*10−2 mg/ml) and (1*10−1 mg/ml) on rats isolated thoracic aortic smooth muscle cells were studied. Green synthesized CuO@Fe3O4@Walnut shell NCs showed effective antivasoconstriction activity against norepinephrine (NE) (1*10−9–10−4 M) induced contraction in endothelium-intact and endothelium-denuded rats aortic smooth muscle. In conclusion, the aqueous extract of C. aronia acts as potential reducing and stabilizing media to the green synthesis of bioactive CuO@Fe3O4@Walnut shell NCs and the NCs play an important role against contractile effects of NE on rats’ aortic smooth muscle.

THERAPEUTIC EFFECTS OF Cratargus azarolus VAR. aronia IN UROLITHIATIC MALE ALBINO RATS

“…Similarly to the current study, Lien et al, (2016) and Mahmud et al, (2021), discovered that in urolithiasis defect the level of serum TG significantly increased. More recently, Al-Mobideen et al, (2022), investigation support current study that C. aronia significantly decreased TG levels in diabetic mice.…”

“…Medicinal plants represent an important part in traditional medicine to treat and prevent numerous health disorders (Arshad et al, 2020, Mahmud, 2021. Different parts of the herbs such as stem, bark, root, leaf, and fruits have been used to treat a broad range of diseases (Mahmud, 2017a, Mahmud, 2017b, in their crude forms as herbal teas, syrups, infusions, ointments, liniments and powders (Saad et al, 2005).…”