“Green” synthesis of Ag2S nanoparticles, study of their properties and bioimaging applications

Green synthesis of silver nanoparticles using aqueous extract of hot chili pepper fruits and its antimicrobial activity against Pseudomonas aeruginosa

“…It is generally accepted that metallic nanoparticles can be obtained through laser ablation, chemical, thermal or photo reduction of various compounds and evaporation-condensation approach [3]. Still, different types of nanomaterials, including Ag-NPs can be manufactured through "green synthesis" approach, which is considered superior to the abovementioned techniques due to the simplicity and biosafety because of absence of hazardous materials and toxic intermediates [4,5].…”

Biogenic Sulfur-Based Chalcogenide Nanocrystals: Methods of Fabrication, Mechanistic Aspects, and Bio-Applications

“…Although the applied electric currents hindered their growth, the cells consumed higher amounts of glucose owing to DNA changes, produced more proteins, and increased their cell wall permeability. Furthermore, Ag 2 S NPs synthesized using Pleurotus ostreatus presented good antimicrobial effects and luminescent properties in the green region [182].…”

“…On the other hand, the presence of precursors is necessary when employing fungi to produce released enzymes and peptides that participate in the extracellular NP synthesis. An additional complication lies in the variability of the NP synthesis kinetics as the process may complete within a few hours or last up to several days, depending on several parameters, such as the used organism and species, its growth stage, and sufficient production of key biomolecules involved in the process [92,168,169,175,178,180,182].…”

“…Imaging plant tissues using bioproduced S-NPs is also possible. For example, Borovaya et al imaged the Allium cepa (garlic) epidermal root cells using bioproduced Ag 2 S NPs since these cells emitted luminescence in the green region (520-550 nm) [182].…”

“…In the first case, filter paper disks containing the test compound, NPs in the present case, at a certain concentration are placed on the agar surface whereas in the second, holes are punched in the agar and filled with the test compound [230]. The antimicrobial activity starts with the interaction between NPs and the cell wall of the microbes [84,88,182]. At this level, electrostatic interactions occur by the difference in electrical charges of the negatively charged cell membrane and NPs.…”