Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity

Murugesan, S.; Bhuvaneswari, S.; Sivamurugan, Vajiravelu

doi:10.22159/ijpps.2017v9i5.17105

Cited by 57 publications

(30 citation statements)

References 33 publications

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“…Similar results were observed for silver nanoparticles performed by Asha et al [27] and Rajeshkumar and Malarkodi [28] who got an absorbance peak in the range of 400 to 450 nm when synthesized from different sources. The XRD analysis proves the crystalline nature of the nanoparticles, similar results were also observed for Sivaramakrishnan et al [29,30] and from the Debye-Scherrer equation, the average size of silver nanoparticles was found to be 34 nm. The FTIR analysis gave information about the functional groups of the phytochemicals probably present in the plant extract involved in the reduction of the Ag nanoparticles.…”

Section: Discussionsupporting

confidence: 87%

Green Synthesis of Silver Nanoparticles Using Medicinal Plant Acalypha Indica Leaf Extracts and Its Application as an Antioxidant and Antimicrobial Agent Against Foodborne Pathogens

2017

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Objective: In the present study, silver (Ag) nanoparticles was synthesized by traditionally used medicinal plant Acalypha indica, which was characterized using various advanced tools, and its antioxidant as well as antimicrobial properties, was studied against food pathogens. Methods:The synthesis of silver (Ag) nanoparticles from the leaf extracts were monitored with the characterization of silver nanoparticles with the help of UV-visible spectrophotometer. The optimized time for the synthesis of nanoparticles was 3 h, followed by fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), surface emission microscopy analysis (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM) and energy dispersive spectroscopy analysis (EDX). The antioxidant activity was evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing power assay, while antifungal activity was performed against food pathogens by measuring the zone of inhibition values (ZOI). Results:The Ag nanoparticle produced, have an average particle size of 34 nm with a spherical shape, analyzed from the XRD studies, and size was confirmed with the SEM and TEM analysis. The FTIR analysis gave information about the possible compounds adsorbed on the surface of the Ag nanoparticles. The Ag nanoparticles had good reducing power than the standard and the IC50 value for Ag nanoparticles was 5 mg/ml, while the standard taken had an IC50 value of nearly 6-7 mg/ml. The fungal strain A. fumigates showed ZOI of 133% at 75 µl of concentration proving that Ag nanoparticles can act effectively against this strain when compared to other strains even at low concentrations. Conclusion:The produced Ag nanoparticles can be used for its therapeutic purposes and for large-scale synthesis in food industries for food preservation or packaging.

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Section: Discussionsupporting

confidence: 87%

Green Synthesis of Silver Nanoparticles Using Medicinal Plant Acalypha Indica Leaf Extracts and Its Application as an Antioxidant and Antimicrobial Agent Against Foodborne Pathogens

2017

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“…The standard drug (ofloxacin) (5 µg/disc) observed 19 and 12 mm against S. aureus and E. coli. Nanoparticles may infiltrate the cells causing intracellular loss leading to cell death, and this inhibition depends on the concentration of the AgNPs [35,36]. The observed value of inhibition is in good agreement with recently reported values against E. coli and S. aureus using inorganic nanoparticles by our group [26,37].…”

Section: Antibacterial Activity Of Agnpssupporting

confidence: 91%

Psidium Guajava: A Novel Plant in the Synthesis of Silver Nanoparticles for Biomedical Applications.

Sharmila¹,

Ranjith²,

B³

2018

Asian J Pharm Clin Res

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Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens. Methods:Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and ecofriendly nanoparticles, which could be used in biomedical applications.

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“…The different researchers have reported antibacterial property of different metal NPs [32][33][34][35]. We have tested antibacterial activity of the ZnO NPs and ZnO-GO nanocomposites on Gram-positive (S. epidermidis) and Gram-negative (E. coli) bacteria using agar well diffusion method.…”

Section: Antibacterial Studymentioning

confidence: 99%

Synthesis, Characterization, and Antibacterial Study of Zinc Oxide-Graphene Nanocomposites

Kumar

Kumari

2017

Asian J Pharm Clin Res

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Objectives: A novel facile synthesis of zinc oxide (ZnO) and zinc-graphene oxide nanocomposites (ZnGONC) was achieved by modified sol-gel technique for their pharmaceutical and therapeutic use. Materials and Methods:Spherical, crystalline, defect-free Zinc oxide nanoparticles (ZnO NPs) with diameter 70-90 nm were synthesized by modified sol-gel technique. Reduced graphene oxide was synthesized by modified Hummers method. ZnGONC were synthesized by in situ method. The crystalline nature, size, shape, and dimensions of the NPs, graphene oxide, and nanocomposites were studied by X-ray diffraction method. Transmission electron microscopy analysis was carried out to examine the morphology of NPs and nanocomposites. Results:Fourier transform infrared spectroscopy analysis confirms that the ZnO NPs are surrounded by oxygen and silicon atoms. Antibacterial activity of ZnO NPs and ZnGONC was investigated against Gram-positive and Gram-negative bacteria. Zone of inhibition shown by ZnO NPs and ZnGONC was found to be higher than six investigated standard antibiotics. Conclusion:Synthesized ZnO NPs and nanocomposites can be used as antibacterial agents. This eco-friendly method of synthesis of ZnO NPs and ZnGONC could be a viable solution for industrial applications in the future and therapeutic needs.

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Green Synthesis, Characterization of Silver Nanoparticles of a Marine Red Alga Spyridia Fusiformis and Their Antibacterial Activity

Cited by 57 publications

References 33 publications

Green Synthesis of Silver Nanoparticles Using Medicinal Plant Acalypha Indica Leaf Extracts and Its Application as an Antioxidant and Antimicrobial Agent Against Foodborne Pathogens

Green Synthesis of Silver Nanoparticles Using Medicinal Plant Acalypha Indica Leaf Extracts and Its Application as an Antioxidant and Antimicrobial Agent Against Foodborne Pathogens

Psidium Guajava: A Novel Plant in the Synthesis of Silver Nanoparticles for Biomedical Applications.

Synthesis, Characterization, and Antibacterial Study of Zinc Oxide-Graphene Nanocomposites

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