Green synthesis, characterization and antioxidant activity of bimetallic (Ag-ZnO) nanoparticles using Capparis zeylanica leaf extract

Suresh, P.K.; Doss, A.; Pole, R.P. Praveen; Devika, M.

doi:10.1007/s13399-023-03743-7

Cited by 21 publications

(4 citation statements)

References 47 publications

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“…However, at low concentrations, Ag NPs were found to be toxic, but when combined with copper nanoparticles (CuO NPs) to form what is known as bimetallic NPs the toxicity and the dangerous effect will be reduced. In this study, we attempted to lower the toxicity of the prepared Ag NPs by combining them with CuO NPs as one alloy as mentioned in our recent published bimetallic NPs papers [38][39][40][41][42][43][44][45][46][47][48]. The bond formed between the colistin drug, and the synthesized NPs was intermolecular hydrogen bond as the results confirmed by FTIR analysis in recent publication [49].…”

Section: Synthesis Of Col-ag Nps Col-cuo Nps and Col-ag-cuo Npsmentioning

confidence: 85%

In Vivo and in Vitro activity of colistin-conjugated bimetallic silver-copper oxide nanoparticles against Pandrug-resistant Pseudomonas aeruginosa

Abdul Hak,

Zedan,

El-Mahallawy

et al. 2024

BMC Microbiol

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Background Addressing microbial resistance urgently calls for alternative treatment options. This study investigates the impact of a bimetallic formulation containing colistin, silver, and copper oxide on a pandrug-resistant, highly virulent Pseudomonas aeruginosa (P. aeruginosa) isolate from a cancer patient at the National Cancer Institute, Cairo University, Egypt. Methods Silver nanoparticles (Ag NPs), copper oxide nanoparticles (CuO NPs), and bimetallic silver-copper oxide nanoparticles (Ag-CuO NPs) were synthesized using gamma rays, combined with colistin (Col), and characterized by various analytical methods. The antimicrobial activity of Col-Ag NPs, Col-CuO NPs, and bimetallic Col-Ag-CuO NPs against P. aeruginosa was evaluated using the agar well diffusion method, and their minimum inhibitory concentration (MIC) was determined using broth microdilution. Virulence factors such as pyocyanin production, swarming motility, and biofilm formation were assessed before and after treatment with bimetallic Col-Ag-CuO NPs. The in vivo efficacy was evaluated using the Galleria mellonella model, and antibacterial mechanism were examined through membrane leakage assay. Results The optimal synthesis of Ag NPs occurred at a gamma ray dose of 15.0 kGy, with the highest optical density (OD) of 2.4 at 375 nm. Similarly, CuO NPs had an optimal dose of 15.0 kGy, with an OD of 1.5 at 330 nm. Bimetallic Ag-CuO NPs were most potent at 15.0 kGy, yielding an OD of 1.9 at 425 nm. The MIC of colistin was significantly reduced when combined with nanoparticles: 8 µg/mL for colistin alone, 0.046 µg/mL for Col-Ag NPs, and 0.0117 µg/mL for Col-Ag-CuO NPs. Bimetallic Col-Ag-CuO NPs reduced the MIC four-fold compared to Col-Ag NPs. Increasing the sub-inhibitory concentration of bimetallic nanoparticles from 0.29 × 10-2 to 0.58 × 10-2 µg/mL reduced P. aeruginosa swarming by 32–64% and twitching motility by 34–97%. At these concentrations, pyocyanin production decreased by 39–58%, and biofilm formation was inhibited by 33–48%. The nanoparticles were non-toxic to Galleria mellonella, showing 100% survival by day 3, similar to the saline-treated group. Conclusions The synthesis of bimetallic Ag-CuO NPs conjugated with colistin presents a promising alternative treatment for combating the challenging P. aeruginosa pathogen in hospital settings. Further research is needed to explore and elucidate the mechanisms underlying the inhibitory effects of colistin-bimetallic Ag-CuO NPs on microbial persistence and dissemination.

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Section: Synthesis Of Col-ag Nps Col-cuo Nps and Col-ag-cuo Npsmentioning

confidence: 85%

In Vivo and in Vitro activity of colistin-conjugated bimetallic silver-copper oxide nanoparticles against Pandrug-resistant Pseudomonas aeruginosa

Abdul Hak,

Zedan,

El-Mahallawy

et al. 2024

BMC Microbiol

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“…Herbal products are enriched with pharmacologically active compounds. Since ancient times, plants have been utilized for diverse purposes, including medicines, dyes, cosmetics, disinfectants, and industrial applications [ 10 ]. Clinopodium vulgare L. (Lamiaceae), commonly known as wild basil, is a well-known medicinal plant used in traditional medicines worldwide.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Zinc Oxide Nanoparticles at Different pH Values from Clinopodium vulgare L. and Their Assessment as an Antimicrobial Agent and Biomedical Application

et al. 2023

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The current study attempts to evaluate the formation, morphology, and physico-chemical properties of zinc oxide nanoparticles (ZnO NPs) synthesized from Clinopodium vulgare extract at different pH values and to investigate their antimicrobial and biomedical application potential. The reduction of zinc ions to ZnO NPs was determined by UV spectra, which revealed absorption peaks at 390 nm at pH 5 and 348 nm at pH 9, respectively. The spherical morphology of the nanoparticles was observed using scanning electron microscopy (SEM), and the size was 47 nm for pH 5 and 45 nm for pH 9. Fourier-transformed infrared spectroscopy (FTIR) was used to reveal the presence of functional groups on the surface of nanoparticles. The antibacterial activity was examined against Staphylococcus aureus, Streptococcus pyogenes, and Klebsiella pneumonia via the agar-well diffusion method. Comparatively, the highest activities were recorded at pH 9 against all bacterial strains, and among these, biogenic ZnO NPs displayed the maximum inhibition zone (i.e., 20.88 ± 0.79 mm) against S. aureus. ZnO NPs prepared at pH 9 exhibited the highest antifungal activity of 80% at 25 mg/mL and antileishmanial activity of 82% at 400 mg/mL. Altogether, ZnO NPs synthesized at pH 9 show promising antimicrobial potential and could be used for biomedical applications.

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“…ZnO NPs internalization can cause membrane disruption and dysfunction . Additionally, the potential use of ZnO NPs as antimicrobial agents has been investigated not only for biomedical devices but also for water remediation , and photocatalysis …”

Section: Introductionmentioning

confidence: 99%

“…ZnO NPs internalization can cause membrane disruption and dysfunction. 18 Additionally, the potential use of ZnO NPs as antimicrobial agents has been investigated not only for biomedical devices 19 but also for water remediation 20 , 21 and photocatalysis. 22 …”

Section: Introductionmentioning

confidence: 99%

Effects of Zn–ZnO Core–Shell Nanoparticles on Antimicrobial Mechanisms and Immune Cell Activation

Fialho,

Costa-Barbosa,

Sampaio

et al. 2023

ACS Appl. Nano Mater.

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The deposition of zinc−zinc oxide nanoparticles (Zn−ZnO NPs) onto porous Ta 2 O 5 surfaces enriched with calcium phosphate by DC magnetron sputtering was investigated to improve the surface antimicrobial activity without triggering an inflammatory response. Different sizes and amounts of Zn NPs obtained by two optimized different depositions and an additional thin carbon (C) layer deposited over the NPs were explored. The deposition of the Zn NPs and the C layer mitigates the surface porosity, increasing the surface hydrophobicity and decreasing the surface roughness. The possible antimicrobial effect and immune system activation of Zn−ZnO NPs were investigated in Candida albicans and macrophage cells, respectively. It was found that the developed surfaces displayed a fungistatic behavior, as they impair the growth of C. albicans between 5 and 24 h of culture. This behavior was more evident on the surfaces with bigger NPs and the highest amounts of Zn. The same trend was observed in both reactive oxygen species (ROS) generation and loss of C. albicans' membrane integrity. After 24 h of culture, cell toxicity was also dependent on the amount of the NPs. Cell toxicity was observed in surfaces with the highest amount of Zn NPs and with the C layer, while cells were able to grow without any signs of cytotoxicity in the porous surfaces with the lowest amount of NPs. The same Zn-dose-dependent behavior was noticed in the TNF-α production. The Zn-containing surfaces show a vastly inferior cytokine secretion than the lipopolysaccharide (LPS)-stimulated cells, indicating that the modified surfaces do not induce an inflammatory response from macrophage cells. This study provides insights for understanding the Zn amount threshold that allows a simultaneous inhibition of the fungi growth with no toxic effect and the main antimicrobial mechanisms of Zn−ZnO NPs, contributing to future clinical applications.

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Green synthesis, characterization and antioxidant activity of bimetallic (Ag-ZnO) nanoparticles using Capparis zeylanica leaf extract

Cited by 21 publications

References 47 publications

In Vivo and in Vitro activity of colistin-conjugated bimetallic silver-copper oxide nanoparticles against Pandrug-resistant Pseudomonas aeruginosa

In Vivo and in Vitro activity of colistin-conjugated bimetallic silver-copper oxide nanoparticles against Pandrug-resistant Pseudomonas aeruginosa

Synthesis and Characterization of Zinc Oxide Nanoparticles at Different pH Values from Clinopodium vulgare L. and Their Assessment as an Antimicrobial Agent and Biomedical Application

Effects of Zn–ZnO Core–Shell Nanoparticles on Antimicrobial Mechanisms and Immune Cell Activation

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