Green synthesis and characterization of nanosilver derived from extracellular metabolites of potent Bacillus subtilis for antifungal and eco-friendly action against phytopathogen

Gajera, H. P.; Hirpara, Darshna G.; Bhadani, Rushita V.; Golakiya, B. A.

doi:10.1007/s10534-022-00382-9

Cited by 7 publications

(6 citation statements)

References 30 publications

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“…The possible formation of NPs from each prepared nanosilver clay composite sample was investigated using a JEOL 2100 TEM (Jeol Ltd., Tokyo, Japan), operated at 200 kV. Nanosilver clay composite samples were prepared for TEM analysis as follows; a small quantity of each nanosilver clay composite sample powder was individually sonicated in 1 mL of 95 % methanol for 5 min [28,30] . The sample solutions were deposited on Cu‐grids with holey carbon film (Sigma‐Aldrich, Johannesburg, South Africa).…”

Section: Methodsmentioning

confidence: 99%

“…Our research group reported a simple, faster, and environmentally friendly microwave‐assisted synthesis method for immobilizing AgNPs on montmorillonite clay surfaces without reducing agents [28] . It has been shown that both the concentration and particle size of AgNPs influence antifungal efficacy [23,24,27] with an average particle size between 7–25 nm inhibiting Fusarium oxysporum, Pythium spinosum , and Botrytis cinerea , and the highest inhibition observed at 0.01 mg/mL [30–32] . In other studies, AgNPs of smaller sizes (3, 5, 8, and 10 nm) and different concentrations (0.0125–0.100 mg/mL) used against Fusarium oxysporum f. sp .…”

Section: Introductionmentioning

confidence: 95%

“…Nanosilver clay composite samples were prepared for TEM analysis as follows; a small quantity of each nanosilver clay composite sample powder was individually sonicated in 1 mL of 95 % methanol for 5 min. [28,30] The sample solutions were deposited on Cu-grids with holey carbon film (Sigma-Aldrich, Johannesburg, South Africa). Particle size distribution analysis was done on the TEM images (circa 200 particles) using ImageJ software (National Institute of Health, Maryland, USA).…”

Section: Characterization Of Nanosilver Clay Compositesmentioning

confidence: 99%

“…[28] It has been shown that both the concentration and particle size of AgNPs influence antifungal efficacy [23,24,27] with an average particle size between 7-25 nm inhibiting Fusarium oxysporum, Pythium spinosum, and Botrytis cinerea, and the highest inhibition observed at 0.01 mg/ mL. [30][31][32] In other studies, AgNPs of smaller sizes (3,5,8, and 10 nm) and different concentrations (0.0125-0.100 mg/mL) used against Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains, [31] showed improved activity with a decrease in particle size and increase in concentration.…”

Section: Introductionmentioning

confidence: 99%

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Antifungal Properties of Nanosilver Clay Composites Against Fungal Pathogens of Agaricus bisporus

Ramakutoane,

Roux‐van der Merwe,

Badenhorst

et al. 2023

ChemistrySelect

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This study aimed to determine the possible control of selected pathogenic and competitive fungi of A. bisporus by three nanosilver clay composites and establish the effective concentrations of the composites for inhibiting pathogens. Nanosilver (AgNPs) clay (zeolite, montmorillonite, and palygorskite) composites were synthesized by a microwave‐assisted surface functionalization technique, and various techniques characterized the products. Zeolite and montmorillonite composites showed uniformly distributed spherical AgNPs with an average size of 3.33 nm and 2.85 nm, respectively, whereas palygorskite presented agglomerated and unevenly distributed AgNPs. The influence of the various composites on 9 fungi, including strains of T. aggressivum f. aggressivum, L. fungicola, C. dendroides, and Mycogone sp., was determined in vitro at different concentrations. At 10 mg/mL, AgNP‐zeolite and AgNP‐montmorillonite inhibited 8 out of 9 pathogens, while AgNP‐palygorskite only inhibited 1 pathogen. The nanosilver clay composites tested against A. bisporus revealed no adverse effects on mycelial growth at any concentrations tested. This study confirms that AgNP‐zeolite and AgNP‐montmorillonite composites have effective antifungal properties and can be used as alternative fungicides against mushroom pathogens without affecting A. bisporus growth. However, further investigation is required to unravel the mechanism of selective antifungal activity observed in this study.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Characterization Of Nanosilver Clay Compositesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antifungal Properties of Nanosilver Clay Composites Against Fungal Pathogens of Agaricus bisporus

Ramakutoane,

Roux‐van der Merwe,

Badenhorst

et al. 2023

ChemistrySelect

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“…The data presented in this study are available in [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ] (MNPs biosynthesis mechanisms), [ 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 ,...…”

mentioning

confidence: 99%

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

et al. 2023

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Nanoparticles (1 to 100 nm) have unique physical and chemical properties, which makes them suitable for application in a vast range of scientific and technological fields. In particular, metal nanoparticle (MNPs) research has been showing promising antimicrobial activities, paving the way for new applications. However, despite some research into their antimicrobial potential, the antimicrobial mechanisms are still not well determined. Nanoparticles’ biosynthesis, using plant extracts or microorganisms, has shown promising results as green alternatives to chemical synthesis; however, the knowledge regarding the mechanisms behind it is neither abundant nor consensual. In this review, findings from studies on the antimicrobial and biosynthesis mechanisms of MNPs were compiled and evidence-based mechanisms proposed. The first revealed the importance of enzymatic disturbance by internalized metal ions, while the second illustrated the role of reducing and negatively charged molecules. Additionally, the main results from recent studies (2018–2022) on the biosynthesis of MNPs using microorganisms were summarized and analyzed, evidencing a prevalence of research on silver nanoparticles synthesized using bacteria aiming toward testing their antimicrobial potential. Finally, a synopsis of studies on MNPs applied to cultural heritage materials showed potential for their future use in preservation.

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Biochemical and molecular depictions to develop ech42 gene-specific SCAR markers for recognition of chitinolytic Trichoderma inhibiting Macrophomina phaseolina (Maubl.) Ashby

et al. 2023

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Green synthesis and characterization of nanosilver derived from extracellular metabolites of potent Bacillus subtilis for antifungal and eco-friendly action against phytopathogen

Cited by 7 publications

References 30 publications

Antifungal Properties of Nanosilver Clay Composites Against Fungal Pathogens of Agaricus bisporus

Antifungal Properties of Nanosilver Clay Composites Against Fungal Pathogens of Agaricus bisporus

Biosynthesis of Metal and Metal Oxide Nanoparticles Using Microbial Cultures: Mechanisms, Antimicrobial Activity and Applications to Cultural Heritage

Biochemical and molecular depictions to develop ech42 gene-specific SCAR markers for recognition of chitinolytic Trichoderma inhibiting Macrophomina phaseolina (Maubl.) Ashby

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