Green Synthesis and Characterization of Silver Nanoparticles for Antimicrobial Activity Against Burn Wounds Contaminating Bacteria

Koirala

et al. 2019

Acta Chemica Malaysia

Plant-mediated synthesis of silver nanoparticles through green chemistry approach has evolved into a new era of research; however, the heterogeneous size and dispersity of silver nanoparticles have limited its applications, such as surface-enhanced Raman scattering, plasmon resonance, and more specifically in the medical field of target drug delivery and therapeutic activity. Here-we investigated the effect of purification and separation modes on the dispersity, size, and morphology of silver nanoparticles, synthesized by plant extract (Nyctanthes arbor-tristis). Transmission electron microscopy revealed silver nanoparticles with an average diameter of 13.0 nm when synthesized through ethanol precipitation, which is advantageously smaller. This result suggests that the silver nanoparticles size can be fine-tuned by changing the separation mode during purification from plant extract. Due to uniformity, our obtained nanoparticles can be expected to show higher catalytic activity towards photochemical reactions, drug delivery and antibacterial activity due to the absence of inactive coating layer (capping agent).

Section: X-ray Diffraction Analysis Of Ag Npssupporting

confidence: 85%

“…Rout et al, also biosynthesized Ag NPs using a leaf extract of N. arbor-tristis with an average size of 20 nm. [52] However, their TEM study used the crude Ag NP colloidal solution without separation or purification, which known to affect the particle size.…”

Section: Introductionmentioning

confidence: 99%

Improved method for separation of silver nanoparticles synthesized using the Nyctanthes arbor-tristis shrub

Koirala

et al. 2019

Acta Chemica Malaysia

“…Juss., 69 Arnebia nobilis Rech.f., 70 Melia dubia Cav., 71 Terminalia chebula Retz., 72 Lansium domesticum Corrêa, 73 Orchidantha chinensis T.L.Wu, 74 A. vera (L.) Burm.f., 75 glucuronoxylan, 76 Momordica charantia L., 77,78 Carica papaya L., 79 Cymbopogon citratus (DC.) Stapf, 80 Nyctanthes arbor-tristis L., 81 Naringi crenulata (Roxb.) Nicolson, 82 Phytophthora infestans, 83 Biophytum sensitivum, 84 Propolis, 85 and Bryonia laciniosa L. 86 Most of these extracts possess inherent antibacterial and antimicrobial activity, which makes them appropriate for wound care.…”

Section: Green-synthesized Metal Nanoparticles Using Plantsmentioning

confidence: 99%

Natural product-based nanomedicines for wound healing purposes: therapeutic targets and drug delivery systems

Hajialyani¹,

Tewari²,

Sobarzo‐Sánchez³

et al. 2018

IJN

167

113

Wound healing process is an intricate sequence of well-orchestrated biochemical and cellular phenomena to restore the integrity of the skin and subcutaneous tissue. Several plant extracts and their phytoconstituents are known as a promising alternative for wound healing agents due to the presence of diverse active components, ease of access, and their limited side effects. The development of nanotechnological methods can help to improve the efficacy of different therapeutics as well as herbal-based products. Here, we present a review of the efficacy of the plant based-nanomaterials in the management of wounds and discuss the involved therapeutic targets. For this purpose, a profound search has been conducted on in vitro, in vivo, and/or clinical evidences evaluating the efficacy and pharmacological mechanisms of natural product-based nanostructures on different types of wounds. Different pharmacological targets are involved in the wound healing effects of herbal-based nanostructures, including suppressing the production of inflammatory cytokines and inflammatory transduction cascades, reducing oxidative factors and enhancing antioxidative enzymes, and promoting neovascularization and angiogenic pathways through increasing the expression of vascular endothelial growth factor, fibroblast growth factor, and platelet-derived growth factor. Moreover, nanostructure of plant extracts and their phytochemicals can enhance their bioavailability, control their release in the form of sustained delivery systems to the wound site, and enhance the permeability of these therapeutics to the underlying skin layers, which are all necessary for the healing process. Overall, various plant extracts and their natural compounds, used in nanoformulations, have demonstrated high activity in the management of wounds and thus can be assumed as future pharmaceutical drugs.

J Nanomedic Nanotechnol

“…After 0 minute of reaction of the yellow colour mixture of AgNO 3 solution with leaf extract was changed black suspended mixture very rapidly at room temperature just after 2 minutes. It was observed that the biosynthesis of SNPs using leaf extracts of Ocimum sanctum was taken place very rapidly than the other plants leaf [39]. The leaf extracts of Ocimum sanctum and concentrations of AgNO 3 solution were also wide-ranging at 5% to 10% and 1 to 4 mM by volume, respectively.…”

Section: Synthesis Of Silver Nanoparticles (Snps)mentioning

confidence: 99%

Biosynthesis and Characterization of Silver Nanoparticles (Snps) by using Leaf Extracts of Ocimum Sanctum L (Tulsi) and Study of its Antibacterial Activities

Bindhani¹,

Panigrahi²

2015

The present study aimed to synthesis of SNPs (Silver Nanoparticles) or (Green-Silver) in aqueous medium using leaf extracts of Ocimum sanctum L. The synthesized silver nanoparticles (SNPs) were characterized by the different techniques such as UV-vis Spectrophotometer, XRD, FTIR and TEM. The synthesis of silver nanoparticles were confirmed due to the SPR optical absorption band peak at ~440 nm by UV-vis spectrophotometer, nearly 15-45 nm in diameter with spherical in shape by TEM, high crystalline with the Bragg peaks of (111), (200), (220) and (311) plane as the predominant orientation by XRD and FTIR confirmed the presence of methoxy and allyl groups in SNPs. The reduction of Ag metal ions was performed due to the presence of various types of anti-oxidants in the leaves of Ocimum sanctum L. and each having a unique structure and functions. The formations of silver nanoparticles through aqueous medium were fairly stable and which can be incubated up to 4 months in refrigerator. At last, the antimicrobial activities of AgNPs were evaluated using different bacteria strain. It was found that, this biosynthesizing of silver nanoparticles may inhibit the all types of bacterial pathogenic organisms competently. This recent research study opens an innovative design to progress our understanding of how SNPs behave can be optimized to improve human antimicrobial activities.Citation: Bindhani BK, Panigrahi AK (2015) Biosynthesis and Characterization of Silver Nanoparticles (SNPs) by using Leaf Extracts of Ocimum Sanctum L (Tulsi) and Study of its Antibacterial Activities.