Green synthesis and characterisation of silver nanoparticles and their effects on antimicrobial efficacy and biochemical profiling in Citrus reticulata

Abstract

The synthesis of nanoparticles by utilising plant extract has revolutionised the field of nanotechnology. In the present study, AgNPs were synthesised by utilising the leaves of as reducing and stabilising agent. UV-visible spectroscopy showed characteristic surface plasmon band in the range of 413-420 nm. Scanning electron microscopy (SEM) elucidated rectangular segments fused together. X-ray diffraction (XRD) analysis confirmed the crystalline nature of AgNPs and presence of metallic silver ions was confirmed by energy dispersive X-ray (EDX). The different concentrations (10, 20, 30 and 40 ppm) of AgNPs were exogenously applied on to record the disease incidence at different day intervals. The disease intensity was progressively increased in all the applied treatments with the passage of time. The 30 ppm concentration of AgNPs was found to be most suitable concentration for creating the resistance against brown spot disease. Moreover, the effects of AgNPs were also assessed for biochemical profiling in . The enhanced production of endogenous enzymes and non-enzymatic components was observed in response to 30 ppm concentration of AgNPs. The present work highlighted that green synthesised AgNPs can be as used as biological control of citrus diseases and the enhanced production of secondary metabolites antioxidants.

Assessment of AgNPs exposure on physiological and biochemical changes and antioxidative defence system in wheat ( Triticum aestivum L) under heat stress

“…Mehrian et al [64] explained that the level of antioxidants activities (POX, CAT and APX) was significantly high in tomato plants treated with AgNPs as compared with untreated plants. Similarly, few other researchers [17,21,64,73] reported maximum activities of CAT and peroxidase in castor seedlings, S. polyrhiza, Fenugreek and Citrus in response to AgNPs treatment.…”

Exposure of stevia (Stevia rebaudiana B.) to silver nanoparticles in vitro: transport and accumulation

“…The use of silver nanoparticles (AgNPs) in biological systems is part of the development of bionanotechnology. This discipline offers alternatives to solve problems related to the production of food crops by inhibiting microbial agents 1–3 . In plants, AgNPs have been used to induce germination, increase crop yields and promote development 4,5 .Some studies have focused on the description of antimicrobial activity and hormesis under in vitro conditions, in which development changes and increased biomass production have been observed 6–9 .…”

Silver nanoparticles and silver salt (AgNO3) elicits morphogenic and biochemical variations in callus cultures of sugarcane

“…Mehrian et al [58] explained that the level of antioxidants activities (POX, CAT and APX) was significantly high in tomato plants treated with AgNPs as compared with untreated plants. Similarly, few other researchers [53,71,72] reported maximum activities of CAT and POX in castor seedlings, S. polyrhiza, and Citrus reticulata in response to AgNPs treatment.…”