Silver nanoparticles and silver salt (AgNO₃) elicits morphogenic and biochemical variations in callus cultures of sugarcane

Abstract

The research work was arranged to check the role of AgNPs and silver ions on callus cells of sugarcane (Saccharum spp. cv CP-77,400). AgNPs were synthesized chemically and characterized by UV-Vis spectra, XRD and SEM. AgNPs and silver ions were applied in various concentrations (0, 20, 40, 60 ppm) to sugarcane calli and the induced stress was characterized by studying various morphological and biochemical parameters. AgNPs and silver ions treatments produced high levels of malondialdehyde, proline, proteins, TP and TF contents. Similarly, CAT, SOD and POX activity was also significant in both treatments. The lower concentration of AgNPs and silver ions (20 ppm) provided maximum intracellular GSH level. This work mainly showed effects of AgNPs and silver ions on sugarcane calli in terms of morphological aberrations and cell membrane damage due to severe oxidative stress and production of enhanced levels of enzymatic and non-enzymatic antioxidants as self-defence to tolerate oxidative stress by scavenging reactive oxygen species. These preliminary findings will provide the way to study ecotoxicity mechanism of the metal ions and NPs in medicine industry and in vitro toxicity research. Furthermore, silver ions alone and their chemically synthesised AgNPs can be used for various biomedical applications in future.

Phytotoxic impact of bifunctionalized silver nanoparticles (AgNPs-Cit-L-Cys) and silver nitrate (AgNO3) on chronically exposed callus cultures of Populus nigra L.

“…Similarly, AgNPs at 40 and 60 mg/L caused an enhancement in biomass accumulation in callus cultures of sugarcane ( Saccharum spp.) (Iqbal et al 2019 ). Contrarily, it has been observed that fresh and dry mass of pearl millet ( Pennisetum glaucum L.) seedlings reduced significantly after 24 h exposure to increasing concentration of AgNPs (Khan et al 2019 ).…”

Recent Advances in Chitosan‐Based Metal Nanocomposites for Wound Healing Applications

“…Silver sulfadiazine is notably the gold standard for the treatment of local burns [86]. With the development of nanotechnology, many studies have shown that silver nanoparticles exhibit prolonged and controlled release of silver ions; this can improve biological safety and reduce the frequency of clinical dressing replacements required with the use of silver salts [87]. Silver nanoparticles have strong bactericidal properties that have been observed in both in vivo and in vitro experiments [88][89][90][91].…”