Green electrospun pantothenic acid/silk fibroin composite nanofibers: Fabrication, characterization and biological activity

Fan, Li; Cai, Zengxiao; Zhang, Kuihua; Han, Feng; Li, Jingliang; He, Chuanglong; Mo, Xiumei; Wang, Xungai; Wang, Hongsheng

doi:10.1016/j.colsurfb.2013.12.030

Cited by 48 publications

(40 citation statements)

References 32 publications

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“…Shang et al [25] found the membranous materials prepared with regenerated silk fibroin containing low amounts (17-18%) of ß-sheets were easily degraded in water after pretreatment with proteinase XIV and co-culturing with fibroblasts (human corneal fibroblasts). These conclusions are in agreement with our findings, because regenerated silk fibroin [4][5][6][7][8][9][10][11][12]14,15,19,20,25] has a different inner structure and degradation properties to that of natural silk fibroin. Furthermore, serum contains a variety of enzymes that play synergic and antagonist actions.…”

Section: In Vitro and In Vivo Degradation Of The Silk And Degradationsupporting

confidence: 94%

“…Since sericin may have a stronger immunogenicity, silk fibroin [4][5][6][7][8][9][10][11][12][13][14][15] or degummed raw silk [17][18][19] is used in biomedical research. In this study, raw silk and degummed raw silk were implanted subcutaneously into ten Sprague-Dawley rats.…”

Section: Immunogenicity Of Sericinmentioning

confidence: 99%

“…Degradation of degummed silk [17,18], degummed silkprepared surgical sutures [19], or regenerated silk fibroin [1,2,[4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]25,26] is attributed to the degradation of silk fibroin. While the degradation properties of silk fibroin have been the subject of intensive investigation, the degradation properties of raw silk remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Silk structure and degradation

Liu

Song

Jin

et al. 2015

Colloids and Surfaces B: Biointerfaces

102

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To investigate the structure of silk and its degradation properties, we have monitored the structure of silk using scanning electron microscopy and frozen sections. Raw silk and degummed raw silk were immersed in four types of degradation solutions for 156 d to observe their degradation properties. The subcutaneous implants in rats were removed after 7, 14, 56, 84, 129, and 145 d for frozen sectioning and subsequent staining with hematoxylin and eosin (H.E.), DAPI, Beta-actin and Collagen I immunofluorescence staining. The in vitro weight loss ratio of raw silk and degummed raw silk in water, PBS, DMEM and DMEM containing 10% FBS (F-DMEM) were, respectively, 14%/11%, 12.5%/12.9%, 11.1%/14.3%, 8.8%/11.6%. Silk began to degrade after 7 d subcutaneous implantation and after 145 d non-degraded silk was still observed. These findings suggest the immunogenicity of fibroin and sericin had no essential difference. In the process of in vitro degradation of silk, the role of the enzyme is not significant. The in vivo degradation of silk is related to phagocytotic activity and fibroblasts may be involved in this process to secrete collagen. This study also shows the developing process of cocoons and raw silk.

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Section: In Vitro and In Vivo Degradation Of The Silk And Degradationsupporting

confidence: 94%

Section: Immunogenicity Of Sericinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Silk structure and degradation

Liu

Song

Jin

et al. 2015

Colloids and Surfaces B: Biointerfaces

102

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“…Alternatively, the fibroin fibers can be solubilized which is usually achieved by treatment with either highly concentrated lithium bromide solution (Gil et al, 2013;Han et al, 2014;Karahaliloglu et al, 2014) or ternary solvent solutions like calcium chloride in diluted ethanol (Fan et al, 2014;Okabayashi et al, 2009;Yan et al, 2013). The latter can solubilize the fibroin by binding Ca 2þ to its tyrosine residues disrupting van der Waals forces and hydrogen bonds (Inpanya et al, 2012).…”

Section: Silks Can Be and Must Be Processed In Different Ways For Scamentioning

confidence: 99%

Silks as scaffolds for skin reconstruction

Reimers

Liebsch

Radtke

et al. 2015

Biotech & Bioengineering

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In this short review, we describe the use of high molecular weight proteins produced in the glands of several arthropods-commonly called silks-for the purpose to enhance human skin wound healing. To this end an extensive literature search has been performed, the publications have been categorized concerning silk preparation and application and summarized accordingly: Scaffolds to promote wound healing were prepared by processing the silks in different ways including solubilization of the protein fibers followed by casting or electrospinning. The silk scaffolds were additionally modified by coating or blending with the intention of further functionalization. In several approaches, the scaffolds were also vitalized with skin cells or stem cells. In vitro and in vivo models were implied to test for safety and efficiency. We conclude that silk scaffolds are characterized by an advantageous biocompatibility as well as an impressive versatility rendering them ideally suited for application in wounds. Nevertheless, further investigation is needed to exploit the full capacity of silk in different wound models and to achieve clinical transfer in time.

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“…The polymers included, for example, PCL, [67], poly(L-lactic acid)-co-poly(εcaprolactone) (PLACL, [68]), carboxyethyl chitosan, PVA, [69], chitin [70], cellulose-based materials modified by oxidation [71] or with lysozyme [72], collagen [73], gelatin [74], and hyaluronan [75]. The bioactive substances were, for example, growth factors, such as epidermal growth factor [64], vitamins, such as vitamin C [68], vitamin E [76], and pantothenic acid (vitamin B5; [77]), antioxidants, such as grape seed extract ( [78]) or quinone-based chromenopyrazole [79], antibiotics, such as ciprofloxacin [64], tetracycline [68] or gentamycin [62], and other antimicrobial and wound healing agents, such as silver nanoparticles, dandelion leaf extract [63], Aloe vera [80], or astragaloside IV [74]. In order to enlarge the pore size in nanofibrous scaffolds for cell penetration, silk fibroin was electrospun together with so-called "sacrificial" crystals of ice [67] or NaCl [81,82], that is, crystals which are removed after the electrospinning process.…”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

Bačáková¹,

Pajorová²,

Zikmundová³

et al. 2020

Current and Future Aspects of Nanomedicine

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Nanofibrous scaffolds belong to the most suitable materials for tissue engineering, because they mimic the fibrous component of the natural extracellular matrix. This chapter is focused on the application of nanofibers in skin tissue engineering and wound healing, because the skin is the largest and vitally important organ in the human body. Nanofibrous meshes can serve as substrates for adhesion, growth and differentiation of skin and stem cells, and also as an antimicrobial and moistureretaining barrier. These meshes have been prepared from a wide range of synthetic and nature-derived polymers. This chapter is focused on the use of nature-derived polymers. These polymers have good or limited degradability in the human tissues, which depends on their origin and on the presence of appropriate enzymes in the human tissues. Non-degradable and less-degradable polymers are usually produced in bacteria, fungi, algae, plants or insects, and include, for example, cellulose, dextran, pullulan, alginate, pectin and silk fibroin. Well-degradable polymers are usually components of the extracellular matrix in the human body or at least in other verte brates, and include collagen, elastin, keratin and hyaluronic acid, although some polymers produced by non-vertebrate organisms, such as chitosan or poly(3-hydroxybutyrate-co-3-hydroxyvalerate), are also degradable in the human body.Current and Future Aspects of Nanomedicine 2 microbial infection, and at the same time, they can keep appropriate moisture and gas exchange at the wound site.Nanofibrous scaffolds for skin tissue engineering have been fabricated from a wide range of synthetic and nature-derived polymers, which can be either biostable or degradable within the human body. Biostable synthetic polymers used in nanofiber-based skin regenerative therapies include, for example, polyurethane [2], polydimethylsiloxane [3], polyethylene terephthalate [4], polyethersulfone [5], and also hydrogels such as poly(acrylic acid) (PAA, [6]), poly(methyl methacrylate) (PMMA, [7]), and poly[di(ethylene glycol) methyl ether methacrylate] (PDEGMA, [8]). Degradable synthetic polymers typically include poly(ε-caprolactone) (PCL, [9]) and its copolymers with polylactides (PLCL, [10]), polylactides (PLA, [11]) and their copolymers with polyglycolides (PLGA, [12]), and also so-called auxiliary polymers, such as poly(ethylene glycol) (PEG), poly(ethylene oxide) (PEO, [13]) or poly(vinyl alcohol) (PVA, [14]), which facilitated the electrospinning process and improved the mechanical properties and wettability of the chief polymer. However, the synthetic polymers, although they are well-chemically defined and tailorable, are often bioinert, hydrophobic and thus not promoting cell adhesion, and also not well-adhering to the wound site. Therefore, they need to be combined with other bioactive substances, particularly nature-derived polymers.This chapter is focused on nature-derived polymers used for fabrication of nanofibrous scaffolds for skin tissue engineering and wound healing. The advantages of...

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Green electrospun pantothenic acid/silk fibroin composite nanofibers: Fabrication, characterization and biological activity

Cited by 48 publications

References 32 publications

Silk structure and degradation

Silk structure and degradation

Silks as scaffolds for skin reconstruction

Nanofibrous Scaffolds for Skin Tissue Engineering and Wound Healing Based on Nature-Derived Polymers

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