Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells

Vivek, Raju; Thangam, Ramar; Muthuchelian, K.; Gunasekaran, Palani; Krishnasamy, Kaveri; Kannan, Soundarapandian

doi:10.1016/j.procbio.2012.09.025

Cited by 354 publications

(191 citation statements)

References 25 publications

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“…1). Under the UV region, AgNPs give a characteristic absorbance band due to the excitation mode of their surface plasmon which is dependent on the nanoparticle size (Vivek et al 2012;. The earlier reports have concluded that AgNPs production from fungi extract Rhizopus stolonifer (Banu et al 2011) and plant extract Rhizophora apiculata (Antony et al 2011) showed maximum absorbance at 422 and 423 nm, respectively.…”

Section: Resultsmentioning

confidence: 99%

Toxicity assessment on haemotology, biochemical and histopathological alterations of silver nanoparticles-exposed freshwater fish Labeo rohita

2015

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The increasing use of nano based-products induces the potential hazards from their manufacture, transportation, waste disposal and management processes. In this report, we emphasized the acute toxicity of silver nanoparticles (AgNPs) using freshwater fish Labeo rohita as an aquatic animal model. The AgNPs were synthesized using chemical reduction method and the formation of AgNPs was monitored by UV-Visible spectroscopy analysis. The functional groups, crystaline nature and morphological characterizations were carried out by fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and high resolution transmission electron microscopy (HRTEM) analysis. UV-Vis range was observed at 420 nm and XRD pattern showed that the particles are crystalline nature. HRTEM analysis revealed that the morphology of particles was spherical and size ranges between 50 and 100 nm. This investigation was extended to determine the potential acute toxicity, L. rohita was treated orally with the lethal concentration (LC 50 ) of AgNPs. The antioxidative responses were studied in the three major tissues such as gill, liver and muscle of L. rohita. The results of this investigation showed that increasing the concentration of AgNPs led to bioaccumulation of AgNPs in the major tissues. The haematological parameters showed significant alterations in the treated fish. The histological changes caused by chemically synthesized AgNPs demonstrated the damages in the tissues, primary lamella and blood vessels of L. rohita. The histological study also displayed the formation of vacuolation in liver and muscle when compared with untreated tissues (control) of L. rohita.

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Section: Resultsmentioning

confidence: 99%

Toxicity assessment on haemotology, biochemical and histopathological alterations of silver nanoparticles-exposed freshwater fish Labeo rohita

2015

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“…Hackenberg et al 30 reported that 10 µg/mL of AgNPs reduced human mesenchymal stem cells viability within 1 hour exposure. Vivek et al 22 reported that the cytotoxicity of AgNPs was significantly increased according to the concentration of nanoparticles. Moreover, several studies reported that AgNPs may induce reactive oxygen species and cause damage to cellular components leading to cell death.…”

Section: Cytotoxicitymentioning

confidence: 99%

“…18 Previous studies reported the cytotoxicity of AgNPs against NIH 3T3 fibroblast cells, HeLa cells, human glioblastoma cells, and human breast cancer cells (MCF-7). [19][20][21][22] However, to our knowledge, cytotoxicity of AgNPs in rat splenocytes have never been explored.…”

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confidence: 99%

“…The stock concentration (5 mg/mL) of MTT, a yellow tetrazole, was prepared and 20 µL of MTT was added in each AgNP-treated well and incubated for 4 hours. Purple colored formazan crystals were observed in the bottom of the well and these crystals were dissolved with 200 µL of dimethyl sulfoxide (DMSO), and read at 595 nm 18,22 in a multi-well plate reader (Epoch microplate spectrophotometer; Biotek, Winooski, USA).…”

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confidence: 99%

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Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Aravinthan

Govarthanan

Selvam³

et al. 2015

IJN

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A rapid, green phytosynthesis of silver nanoparticles (AgNPs) using the aqueous extract of Helianthus tuberosus (sunroot tuber) was reported in this study. The morphology of the AgNPs was determined by transmission electron microscopy (TEM). Scanning electron microscopy–energy-dispersive spectroscopy (SEM–EDS) and X-ray powder diffraction (XRD) analysis confirmed the presence of AgNPs. Fourier transform infrared spectroscopy (FTIR) analysis revealed that biomolecules in the tuber extract were involved in the reduction and capping of AgNPs. The energy-dispersive spectroscopy (EDS) analysis of the AgNPs, using an energy range of 2–4 keV, confirmed the presence of elemental silver without any contamination. Further, the synthesized AgNPs were evaluated against phytopathogens such as Ralstonia solanacearum and Xanthomonas axonopodis . The AgNPs (1–4 mM) extensively reduced the growth rate of the phytopathogens. In addition, the cytotoxic effect of the synthesized AgNPs was analyzed using rat splenocytes. The cell viability was decreased according to the increasing concentration of AgNPs and 67% of cell death was observed at 100 μg/mL.

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“…51,52 The absence of cytotoxicity suggests that they may be applied as costeffective and reliable mediators in cancer diagnostics and antibacterial agents in wound healing.…”

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confidence: 99%

Coalescence of functional gold and monodisperse silver nanoparticles mediated by black <em>Panax ginseng</em> Meyer root extract

Wang¹,

Markus²,

Kim³

et al. 2016

IJN

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Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells

Cited by 354 publications

References 25 publications

Toxicity assessment on haemotology, biochemical and histopathological alterations of silver nanoparticles-exposed freshwater fish Labeo rohita

Toxicity assessment on haemotology, biochemical and histopathological alterations of silver nanoparticles-exposed freshwater fish Labeo rohita

Sunroot mediated synthesis and characterization of silver nanoparticles and evaluation of its antibacterial and rat splenocyte cytotoxic effects

Coalescence of functional gold and monodisperse silver nanoparticles mediated by black <em>Panax ginseng</em> Meyer root extract

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