Green-banana biomass consumption by diabetic patients improves plasma low-density lipoprotein particle functionality

Abstract

The aim of this study was to investigate the effects of 6-months consumption of green-banana biomass on the LDL particle functionality in subjects with type 2 diabetes. Subjects (n = 39, mean age 65 years old) of both sexes with diabetes (HbA1c ≥ 6·5%) were randomized to receive nutritional support plus green-banana biomass (40 g) (n = 21) or diet alone (n = 18) for 6-months. Non-linear optical responses of LDL solutions from these participants were studied by Z-scan technique. UV–visible spectrophotometer was used to measure the absorbance of the LDL samples. Small Angle X-ray Scattering and Dynamic Light Scattering experiments were used to look for any structural changes in LDL samples and to determine their size distribution. The Lipoprint test was used to determine the LDL sub-fractions in terms of distribution and size. Consumption of green-banana biomass, reduced total- ( p = 0.010), non-HDL-cholesterol ( p = 0.043), glucose ( p = 0.028) and HbA1c ( p = 0.0007), and also improved the protection of the LDL particle against oxidation, by the increase in carotenoids content in the particles ( p = 0.007). This higher protection against modifications may decrease the risk of developing cardiovascular disease. These benefits of the green-banana biomass encourage the use of resistant starches with potential clinical applications in individuals with pre-diabetes and diabetes.

Effects of Resistant Starch Interventions on Metabolic Biomarkers in Pre-Diabetes and Diabetes Adults

“…However, changes in FBS, hs-CRP and IL-6 were not significant in the RS2 group compared with the control group. In contrast to the previous finding by Heianza and co-workers ( 6 ), both studies done by Lotfollahi and Gargari recorded decreased HbA1c levels after the intervention ( 31 , 32 ).…”

“…However, changes in FBS, hs-CRP and IL-6 were not significant in the RS2 group compared with the control group. In contrast to the previous finding by Heianza and co-workers ( 6), both studies done by Lotfollahi and Gargari recorded decreased HbA1c levels after the intervention (31,32). Although RS2 can improve the glycemic status and inflammatory markers in some individuals with T2DM, more studies are needed to confirm the efficacy of RS2 as an adjunct therapy in diabetes, as reported by Gargari et al (32).…”

“…Diabetic developments are also affected by chemical and diet-related factors ( 63 ). Recently, RS2 in the form of green-banana biomass studied in 39 T2DM patients of both genders for 6 mths showed significantly lower TC, non-HDL-cholesterol, glucose, and HbA1c levels ( p < 0.05), as well as improved the LDL particles protection against oxidation which indirectly assists in lowering cardiovascular disease risk among T2DM patients ( 31 ). Gargari and co-investigators ( 32 ) have conducted a study to determine if RS can react as an alimentary prebiotic for patients with T2DM.…”

“…• RS had no significant effect on SCFA but reduced FFA rebound (p < 0.001) and second-meal glucose (p = 0.002) and insulin responses (p = 0.024). protection against oxidation which indirectly assists in lowering cardiovascular disease risk among T2DM patients (31). Gargari and co-investigators (32) have conducted a study to determine if RS can react as an alimentary prebiotic for patients with T2DM.…”

Changes in lipoproteins associated with lipid-lowering and antiplatelet strategies in patients with acute myocardial infarction

“…Low-density lipoprotein was obtained by preparative sequential ultracentrifugation equipped with a fixed-angle rotor (Hitachi Himac CP 70MX, Tokyo, Japan) as previously reported [ 17 ]. These samples were dialyzed against phosphate-buffered saline (PBS) with ethylenediaminetetraacetic acid (EDTA) to remove the salts.…”