Green and Orange Fluorescent Carbon Dots for Detecting Oral Cancer by Staining Tissue Sections

Peng, Xiaoxiao; Yang, Huaixia; Li, Chenchen; Zhang, Yubing; Chen, Shu; Long, Yunfei

doi:10.1166/jnn.2019.16859

Cited by 6 publications

(6 citation statements)

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“…Carbon dots (CDs) are zero-dimensional carbon particles with particle sizes ranging from 1 to 10 nm. , In the last few years, CDs have become one of the most popular carbon nanomaterials with unique fluorescent properties, low cytotoxicity, easy preparation, and high-water solubility. They have been successfully applied to photocatalysis, ion detection, biosensing, energy research, and photodynamic therapy. − Because of low toxicity, high storage stability, and excellent biocompatibility, CDs were also applied to cell imaging, such as for onion epidermal cells, HepG2, oral tissue, brain tumors (U87 cells), Escherichia coli, , Pseudomonas aeruginosa and Fusarium avenaceum, Mycobacterium tuberculosis and Magnaporthe oryzae, and so forth . In the study on yeast cell imaging, several reports discovered that CDs were widely dispersed in the membrane and cytoplasmic area of yeast, whereas others demonstrated that CDs were conjugated with the nucleus of yeast .…”

Section: Introductionmentioning

confidence: 99%

Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots

Huo

et al. 2020

J. Agric. Food Chem.

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The estimation of yeast viability with B- and N-doped carbon dots (BN-CDs) was investigated in this paper. BN-CDs with a fluorescent quantum yield of 65.47% were prepared by a one-step hydrothermal method. The size distribution of BN-CDs was relatively narrow, with the majority falling within 7.5–8.5 nm, and they were mainly composed of carbon, oxygen, nitrogen, and boron. BN-CDs were shown to have strong and stable fluorescence. They exhibited excitation-independent photoluminescence property, which could avoid the autofluorescence and limitation of the excitation source. Dead and live yeast cells were distinguished well by BN-CD staining in a short time, and there was no strict requirement for light protection. The application of BN-CDs in beer brewing can solve the problem of estimation of yeast viability.

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Section: Introductionmentioning

confidence: 99%

Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots

Huo

et al. 2020

J. Agric. Food Chem.

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“…Some like acridine orang (AO) are even insoluble in water. Several CDs targeting cell nucleus were reported, ,,− which usually provide blue, green, ,− and orange cell images (Table ). These CDs are usually prepared by a microwave-assisted method and hydrothermal methods.…”

Section: Resultsmentioning

confidence: 99%

“…Cell staining is an indispensable tool for cell biology and medical research. , Specific dyes or chemicals that selectively bind to different structures or molecules within cells are commonly used in cell staining for visualizing and identifying cell organelles and assessing their functions . Based on the changes in cell organelles like nucleus sizes and morphologies, various diseases such as tumors, infectious diseases, and genetic diseases have been diagnosed .…”

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confidence: 99%

“…While staining membrane and cytoplasm is important, nucleus staining receives more attention because it contains the genetic material of the cell . Several types of CDs prepared from flower petals, o -diaminobenzene, ethylenediamine and formic acid, isophthalamide and l -cysteine, and ethylene glycol and polyethylene glycol have been successfully applied for nucleus staining and imaging. However, the number of CDs that can be applied for targeting nucleus is still rare .…”

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confidence: 99%

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User-Friendly Multifunctional Red-Emissive Carbon Dots for Rapid Cell Nucleus Staining via Targeting Nuclear Proteins

Sun,

Li,

Ouyang

et al. 2024

Anal. Chem.

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Cytoarchitectural staining is of great importance in disease diagnosis and cell biology research. This study developed user-friendly multifunctional redemissive carbon dots (R-CDs) for rapid cell nucleus staining via targeting nuclear proteins. R-CDs, simply prepared by electrochemical treatment of 1,2,4-benzenetriamine, exhibit strong emission at 635 nm when excited at 507 nm. The R-CDs can rapidly stain the nucleus of human SH-SY5Y, HepG2, and HUH-7 cells with a high signal-to-noise ratio owing to fluorescence enhancement after entering the nucleus. Compared to conventional cytosolic dyes such as Hoechst and DAPI, R-CDs are cheaper, more highly dispersed in water, and more stable (requiring no stringent storage conditions). The R-CDs show stable optical properties with insignificant photobleaching over 7 days and salt resistance up to 2 M of NaCl. More importantly, R-CDs, possessing a positive charge, allow rapid staining of live cells (3 min) and dead cells (10 s) in saline. According to kinetic variation, R-CDs can distinguish live cells from dead cells. Staining exhibits high efficiency in onion epidermal cells, Aspergillus niger, Caenorhabditis elegans, and human spermatozoa. The mechanism for efficient staining is based on their fast accumulation in the nucleus due to their small size and positive charge and strong interaction with nuclear proteins at amino acid residues of histidine and arginine, resulting in fluorescence enhancement by dozens of times. The developed R-CDs do not bind to DNA and would not cause genetic damage and will find various safe applications in biological and medical fields.

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“…[6,7,8,9,10,11,12]. In recent years, significant progress was made in this field, and numerous effective fluorescent probes were developed for sensing and imaging applications [13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29]. Thiols (including biothiol, hydrogen sulfide, and thiophenol) are important compounds with some unique properties such as strong nucleophilicity, coordination ability, and reducing capacity [30,31,32,33,34,35,36,37,38,39,40,41,42].…”

Section: Introductionmentioning

confidence: 99%

Recent Progress in the Development of Fluorescent Probes for Thiophenol

et al. 2019

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Thiophenol (PhSH) belongs to a class of highly reactive and toxic aromatic thiols with widespread applications in the chemical industry for preparing pesticides, polymers, and pharmaceuticals. In this review, we comprehensively summarize recent progress in the development of fluorescent probes for detecting and imaging PhSH. These probes are classified according to recognition moieties and are detailed on the basis of their structures and sensing performances. In addition, prospects for future research are also discussed.

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Green and Orange Fluorescent Carbon Dots for Detecting Oral Cancer by Staining Tissue Sections

Cited by 6 publications

References 0 publications

Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots

Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots

User-Friendly Multifunctional Red-Emissive Carbon Dots for Rapid Cell Nucleus Staining via Targeting Nuclear Proteins

Recent Progress in the Development of Fluorescent Probes for Thiophenol

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