Efficient Imaging of Saccharomyces cerevisiae Based on B- and N-Doped Carbon Dots

Abstract

The estimation of yeast viability with B- and N-doped carbon dots (BN-CDs) was investigated in this paper. BN-CDs with a fluorescent quantum yield of 65.47% were prepared by a one-step hydrothermal method. The size distribution of BN-CDs was relatively narrow, with the majority falling within 7.5–8.5 nm, and they were mainly composed of carbon, oxygen, nitrogen, and boron. BN-CDs were shown to have strong and stable fluorescence. They exhibited excitation-independent photoluminescence property, which could avoid the autofluorescence and limitation of the excitation source. Dead and live yeast cells were distinguished well by BN-CD staining in a short time, and there was no strict requirement for light protection. The application of BN-CDs in beer brewing can solve the problem of estimation of yeast viability.

The structural characteristics and mechanisms of antimicrobial carbon dots: a mini review

“…Thakur et al first combined ciprofloxacin with CDs to form a Cipro@C-dots conjugate, which could perform in vivo imaging of Saccharomyces cerevisiae (yeast) and emit bright green fluorescence. 71 Similar imaging of CDs in living fungi was also observed in Valsa Mali Miyabe et Yamada, 87 Aspergillus aculeatus, 88,89 Saccharomyces cerevisiae, 90 Colletotrichum gloeosporioides, 91 and Fusarium oxysporum. 92 When CDs were prepared with bacteria as a precursor, the imaging application of CDs can be observed in dead fungal cells Saccharomyces cerevisiae (yeast) and Trichoderma reesei.…”

Reusable flexible poly(vinyl alcohol)/chitosan‐based polymer carbon dots composite film for acid blue 93 dye adsorption

“…Figure 2b shows the absorption peak at 3456 cm −1 corresponding to the N–H or O–H stretching vibrations. The peak at 2875 cm −1 was ascribed to the C–H stretching vibrations, while the sharp peak at 1788 cm −1 was observed due to the C=O stretching vibrations in the carboxyl group [36]. The peak at 1645 cm −1 was assigned to the characteristic amide bond (O=C–NH–) [37].…”

B- and N-doped carbon dots by one-step microwave hydrothermal synthesis: tracking yeast status and imaging mechanism

“…The N 1 s band was convoluted into two peaks at 399.1 and 398.35 eV, representing N–H and N–C, respectively. O 1 s XPS spectrum was decomposed into peaks at 530.6 and 529.5 eV, corresponding to O = C and O–C, respectively [ 49 ]. The spectrum of B 1 s exhibited two peaks at 190.8 and 190.2 eV, which could be assigned to B–C and B–O, respectively [ 42 ].…”