Gravity‐enforced microtissue engineering

Strube, Randy; Haugstetter, Johannes; Furter, Markus; Fernández, A. Nuñez; Fluri, David; Kelm, Jens M.

doi:10.1002/9781118851647.ch2

Cited by 1 publication

(1 citation statement)

References 22 publications

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“…However, a necrotic core was observed over time in MSCs spheroids (200-400 µm), with or without magnetic stimulation, which can be associated with oxygen and nutrients depletion and accumulation of waste products in their core. This effect has been described in the literature before, limiting cell spheroids maximum diameter, depending on cell type [34][35][36].…”

Section: Magnetic Forces Impact On 2d and 3d Cultures Of Mscsmentioning

confidence: 59%

Magnetic stimulation of the angiogenic potential of mesenchymal stromal cells in vascular tissue engineering

Manjua

Portugal

Ferreira

2021

Science and Technology of Advanced Materials

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The growing prevalence of vascular diseases worldwide has emphasized the need for novel tissueengineered options concerning the development of vascularized 3D constructs. This study reports, for the first time, the use of external magnetic fields to stimulate mesenchymal stromal cells (MSCs) to increase the production of vascular endothelial growth factor-A (VEGF-A). Polyvinylalcohol and gelatin-based scaffolds, containing iron oxide nanoparticles, were designed for optimal cell magnetic stimulation. While the application of static magnetic fields over 24 h did not impact on MSCs proliferation, viability and phenotypic identity, it significantly increased the production of VEGF-A and guided MSCs morphology and alignment. The ability to enhance MSCs angiogenic potential was demonstrated by the increase in the number of new vessels formed in the presence of MSCs conditioned media through in vitro and in vivo models. Ultimately, this study uncovers the potential to manipulate cellular processes through short-term magnetic stimulation.

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Section: Magnetic Forces Impact On 2d and 3d Cultures Of Mscsmentioning

confidence: 59%