Graphitic carbon nitride-based nanocomposites electrochemiluminescence systems and their applications in biosensors

Zou, Rui; Teng, Xu; Lin, Yanjun; Lu, Chao

doi:10.1016/j.trac.2020.116054

Cited by 62 publications

(23 citation statements)

References 134 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electrochemical impedance spectroscopy (EIS) was used for the stepwise preparation of the proposed ratiometric ECL biosensor (Figure C). The semicircular part of EIS was related to the electron transfer resistance (Ret), related to the difficulty of electron transfer of a ferricyanide-redox probe between the electrode and the solution. , Because the electroactive ions are transported rapidly to the electrode interface, a very small semicircle was acquired from the bare GCE (curve a). After Zn-MOFs were modified onto the GCE, the Ret increased duo to the poor conductivity of Zn-MOFs (curve b).…”

Section: Resultsmentioning

confidence: 99%

Zinc–Metal Organic Frameworks: A Coreactant-free Electrochemiluminescence Luminophore for Ratiometric Detection of miRNA-133a

et al. 2021

View full text Add to dashboard Cite

Developing a coreactant-free ratiometric electrochemiluminescence (ECL) strategy based on a single luminophore to achieve more accurate and sensitive microRNA (miRNA) detection is highly desired. Herein, utilizing zinc−metal organic frameworks (Zn-MOFs) as the single luminophore, a novel dualpotential ratiometric ECL biosensor was constructed for ultrasensitive detection of miRNA-133a. The as-prepared Zn-MOFs exhibited simultaneous cathode and anode ECL emission. Furthermore, the Zn-MOFs were confirmed to be a multichannel ECL sensing platform with excellent annihilation and coreactant ECL emission. The corresponding ECL behaviors were investigated in detail. Benefiting from the hybridization chain reaction (HCR) amplification technology, N,N-diethylethylenediamine (DEAEA) was modified on hairpin DNA, and the gained products loaded with quantities of DEAEA enhanced the anodic ECL intensity of Zn-MOFs. In the presence of miRNA-133a, the ECL intensity ratio of anode to cathode (I a /I c ) was significantly increased, which realized the ultrasensitive ratiometric detection of miRNA-133a. In addition, without an exogenous coreactant, the biosensor revealed superb accuracy and stability. Under optimal conditions, the detection linearity of miRNA-133a was from 50 aM to 50 fM with a low detection limit of 35.8 aM (S/N = 3). This is the first work to use Zn-MOFs as a single emitter for reliable ratiometric ECL bioanalysis, which provides a new perspective for fabricating a ratiometric ECL biosensor platform.

show abstract

Section: Resultsmentioning

confidence: 99%

Zinc–Metal Organic Frameworks: A Coreactant-free Electrochemiluminescence Luminophore for Ratiometric Detection of miRNA-133a

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Electrochemiluminescence (ECL) is a special chemiluminescence phenomenon based on electrochemistry, which is a process of generating electrochemical species and forming the excited state of a luminophore. − ECL has attracted great attention because of its high sensitivity, easy controllability, rapidness, and a wide dynamic range. , In recent years, with the rapid development of nanobiotechnology, ECL has been widely used in environmental detection, biomedicine, food analysis, and immunosensing fields. , However, so far, there are few studies on the recognition of chiral molecules by ECL because of the absence of suitable chiral materials with good ECL activity.…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Chiral CuO/CoO Nanofibers: An Enhanced Electrochemiluminescence Sensing Strategy for Detection of 3,4-Dihydroxy-Phenylalanine Enantiomers

Song

Hai

et al. 2021

Anal. Chem.

View full text Add to dashboard Cite

l-3,4-Dihydroxy-phenylalanine (l-DOPA) is the most effective drug for the treatment of Parkinson’s disease, which plays a very important role in clinical and neurochemistry. However, how to achieve high-sensitivity recognition of l-DOPA still faces challenges. Here, a facile strategy is presented to construct nitrogen-doped chiral CuO/CoO nanofibers (N-CuO/CoO NFs) with nanozyme activity and electrochemiluminescence property, in which CuO/CoO NFs are used as the catalytic activity center and chiral cysteine (Cys) is used as the inducer of chiral recognition, for enantioselective catalysis and sensitive recognition of DOPA enantiomers. Notably, N doping not only enhances the enzyme-mimic activity of CuO/CoO NFs but also amplifies their electrochemiluminescence (ECL) signals in the presence of luminol. More importantly, in the presence of DOPA enantiomers, the d-cysteine (d-Cys)-modified N-CuO/CoO NFs exhibit different ECL performances; thus, d-Cys@N-CuO/CoO NFs could selectively distinguish and sensitively detect l-DOPA through ECL signals, and the detection limit is 0.29 nM for l-DOPA. In addition, it also showed good sensing performance for the determination of l-DOPA in fetal bovine serum. This is the first report on the detection of DOPA enantiomers based on an enhanced ECL strategy, providing a robust pathway for chiral discrimination and detection of chiral molecules.

show abstract

“…Other types of nanomaterials have recently been used in ECL bioanalysis, such as graphite-phase carbon nitride (CN) [ 74 ], a novel nitrogen-rich two-dimensional carbon material, which has a wide range of applications in photocatalysis and biosensing [ 75 , 76 ]. Surface-modified CN is of great significance due to its practical application, especially for the regulation of its ECL characteristics [ 77 , 78 ].…”

Section: Efficient Ecl Luminophoresmentioning

confidence: 99%

Strategies for Enhancing the Sensitivity of Electrochemiluminescence Biosensors

et al. 2022

View full text Add to dashboard Cite

Electrochemiluminescence (ECL) has received considerable attention as a powerful analytical technique for the sensitive and accurate detection of biological analytes owing to its high sensitivity and selectivity and wide dynamic range. To satisfy the growing demand for ultrasensitive analysis techniques with high efficiency and accuracy in complex real sample matrices, considerable efforts have been dedicated to developing ECL strategies to improve the sensitivity of bioanalysis. As one of the most effective approaches, diverse signal amplification strategies have been integrated with ECL biosensors to achieve desirable analytical performance. This review summarizes the recent advances in ECL biosensing based on various signal amplification strategies, including DNA-assisted amplification strategies, efficient ECL luminophores, surface-enhanced electrochemiluminescence, and ratiometric strategies. Sensitivity-enhancing strategies and bio-related applications are discussed in detail. Moreover, the future trends and challenges of ECL biosensors are discussed.

show abstract

Graphitic carbon nitride-based nanocomposites electrochemiluminescence systems and their applications in biosensors

Cited by 62 publications

References 134 publications

Zinc–Metal Organic Frameworks: A Coreactant-free Electrochemiluminescence Luminophore for Ratiometric Detection of miRNA-133a

Zinc–Metal Organic Frameworks: A Coreactant-free Electrochemiluminescence Luminophore for Ratiometric Detection of miRNA-133a

Nitrogen-Doped Chiral CuO/CoO Nanofibers: An Enhanced Electrochemiluminescence Sensing Strategy for Detection of 3,4-Dihydroxy-Phenylalanine Enantiomers

Strategies for Enhancing the Sensitivity of Electrochemiluminescence Biosensors

Contact Info

Product

Resources

About