Nitrogen-Doped Chiral CuO/CoO Nanofibers: An Enhanced Electrochemiluminescence Sensing Strategy for Detection of 3,4-Dihydroxy-Phenylalanine Enantiomers

Abstract

l-3,4-Dihydroxy-phenylalanine (l-DOPA) is the most effective drug for the treatment of Parkinson’s disease, which plays a very important role in clinical and neurochemistry. However, how to achieve high-sensitivity recognition of l-DOPA still faces challenges. Here, a facile strategy is presented to construct nitrogen-doped chiral CuO/CoO nanofibers (N-CuO/CoO NFs) with nanozyme activity and electrochemiluminescence property, in which CuO/CoO NFs are used as the catalytic activity center and chiral cysteine (Cys) is used as the inducer of chiral recognition, for enantioselective catalysis and sensitive recognition of DOPA enantiomers. Notably, N doping not only enhances the enzyme-mimic activity of CuO/CoO NFs but also amplifies their electrochemiluminescence (ECL) signals in the presence of luminol. More importantly, in the presence of DOPA enantiomers, the d-cysteine (d-Cys)-modified N-CuO/CoO NFs exhibit different ECL performances; thus, d-Cys@N-CuO/CoO NFs could selectively distinguish and sensitively detect l-DOPA through ECL signals, and the detection limit is 0.29 nM for l-DOPA. In addition, it also showed good sensing performance for the determination of l-DOPA in fetal bovine serum. This is the first report on the detection of DOPA enantiomers based on an enhanced ECL strategy, providing a robust pathway for chiral discrimination and detection of chiral molecules.

Nanozyme‐encoded luminescent detection for food safety analysis: An overview of mechanisms and recent applications

“…Nitrogen‐doped chiral CuO/CoO nanofibers were prepared as POD‐like nanozymes with an electrochemiluminescence (ECL) property. This D‐cysteine (Cys)‐modified nanozyme exhibited varying ECL performances in the presence of dihydroxyphenylalanine enantiomers (Song et al., 2021).…”

CoO Nanozymes with Multiple Catalytic Activities Regulate Atopic Dermatitis

“…However, in previous studies, CoO nanoparticles have often been doped with other substances as part of the catalyst. Few studies have used CoO nanoparticles alone as catalysts to participate in the reaction, and most of the related enzyme-like catalytic activities were peroxidase-like or oxidase-like [53][54][55][56]. In this paper, we prepared CoO nanoparticles with a "cluster" morphology, and the morphology of CoO nanoparticles can be changed by changing the reaction temperature.…”

“…Co 3 O 4 nanozymes are widely used in cancer therapy, antiviral, magnetic resonance imaging (MRI), and targeted drug delivery [49][50][51]. At present, many studies have reported that CoO nanozymes with POD-like activity prepared by a variety of synthetic methods have extremely high application value in biosensing [52][53][54][55]; CoO nanostructures have the advantages of high stability, large specific surface area, and easy redox [56,57]. However, pure CoO nanoparticles show poor electrical conductivity and tend to self-aggregate to cover active sites.…”

Acetylcholinesterase Activity Monitoring and Natural Anti-neurological Disease Drug Screening via Rational Design of Deep Eutectic Solvents and CeO2-Co(OH)2 Nanosheets

“…Nanozymes with oxidase/peroxidase activity play a critical role in the oxidation reaction of colorless TMB to blue ox-TMB. So far, the reported nanozymes mainly include carbon-based nanomaterials (for example, graphene oxide, carbon nanotubes, , and carbon nanodots), metals (such as Au, , Pt, Ag, and Ni), metal oxides (such as CeO 2 , MnO 2 , CuO, and Mn 3 O 4 ), and other nanocomposites (such as Co 3 O 4 @CeO 2 , TiO 2 @CeOx, NPG/Cu 2 O, and magnetic nanoporous graphene). Among them, nanocomposites exhibit excellent catalytic performance due to their strong synergistic effect and rich hybrid connection with redox relationships .…”