Graphene Oxide/α-MnO₂ Binary Nanosheets Based Non-Enzymatic Biosensor for Pico-Molar Level Electrochemical Detection of Biomarker (Guanine) in DNA Sample

Abstract

We reported Graphene Oxide/α-MnO 2 binary nanosheets (GO/α-MnO 2 BNSs) Based Non-Enzymatic Biosensor for the detection of guanine using selective and ultra-sensitive electrochemical method. In this work, GO/α-MnO 2 BNSs synthesized using simple and cost-effective ultra-sonication method. As produced GO/α-MnO 2 BNSs were investigated by FE-SEM, TEM, EDX, FT-IR, XRD, EIS and electrochemical methods. The electrochemical oxidation behavior of guanine at the GO/α-MnO 2 BNSs modified electrode was evaluated by cyclic voltammetry (CV) while sensitive guanine detection was performed via Differential pulse voltammetry. The as-fabricated biosensor exhibited lower limit of detection was 23.9 (± 0.52) pM with excellent sensitivity about 8.835 (± 0.96) μA μM −1 cm −2 (R 2 = 0.995) and linear range of 50 nM−10.5 μM without surface fouling. The sensor was evidently examined for the detection of guanine in the real sample of DNA to predict unknown concentrations of guanine in the pH 5.0 phosphate buffer.

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