Abstract:In this study, a label-free detection approach for effective detection of Odontoglossum ringspot virus (ORSV) in orchids was developed. We used a semiconductor fabrication process to fabricate 1,810 micro/nano hybrid-structured sensing electrodes on an 8-inch reclaimed silicon wafer. The commonly used self-assembled monolayer (SAM) process was then employed to sequentially modify the electrode surface with 11-mercaptoundecanoic acid (11-MUA), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccin… Show more
“…To date, many researchers have adapted antibody-modified electrochemical biosensors for virus detection. 148,149 Wang et al fabricated AuNP-based micro/nano hybrid-structured sensing electrodes to detect Odontoglossum ringspot virus (ORSV). 148 The electrodes were first modified with a self-assembled monolayer (SAM) of anti-ORSV antibodies and electrochemical impedance spectroscopy (EIS) changes were monitored for ORSV quantification.…”
Many outbreaks of emerging disease (e.g., avian influenza, SARS, MERS, Ebola, COVID-19) are caused by viruses. In addition to direct person-to-person transfer, the movement of these viruses through environmental matrices...
“…To date, many researchers have adapted antibody-modified electrochemical biosensors for virus detection. 148,149 Wang et al fabricated AuNP-based micro/nano hybrid-structured sensing electrodes to detect Odontoglossum ringspot virus (ORSV). 148 The electrodes were first modified with a self-assembled monolayer (SAM) of anti-ORSV antibodies and electrochemical impedance spectroscopy (EIS) changes were monitored for ORSV quantification.…”
Many outbreaks of emerging disease (e.g., avian influenza, SARS, MERS, Ebola, COVID-19) are caused by viruses. In addition to direct person-to-person transfer, the movement of these viruses through environmental matrices...
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