Graphene oxide nanoparticles induce hepatic dysfunction through the regulation of innate immune signaling in zebrafish (<i>Danio rerio</i>)

Xiong, Gaofeng; Deng, Yunyun; Liao, Xinjun; Zhang, June; Cheng, Bo; Cao, Zigang; Lu, Huiqiang

doi:10.1080/17435390.2020.1735552

Cited by 30 publications

(26 citation statements)

References 56 publications

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“…They were probably the effective ingredients of SNX in reducing dyslipidemia. In addition, GO annotation revealed that SNX regulated lipid metabolism in relation to steroid metabolic process, cholesterol storage, myelin sheath, steroid hormone receptor activity and steroid binding 44,45 . Oxidoreductase activity might play an important role in the mechanism of SNX, which was proved in this study.…”

Section: Discussionsupporting

confidence: 64%

Shunaoxin pills improve the antihypertensive effect of nifedipine and alleviate its renal lipotoxicity in spontaneous hypertension rats

Liu

Man

Luo

et al. 2020

Environmental Toxicology

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Shunaoxin pills (SNX) have been used to treat cerebrovascular diseases in China since 2005. Hypertension is a major risk factor for cerebrovascular disease. This study aimed to explore the synergistic antihypertensive effect of SNX and nifedipine and whether SNX could alleviate nifedipine‐induced renal lipotoxicity. During administration, systolic blood pressure was measured weekly. After 5 weeks administration, we examined pathological changes of kidney, renal function, the lipid metabolism index, and adipogenesis genes expression in the kidney tissues, and explored its underlying mechanism. Finally, network pharmacology was used for supplement and verification. As a result, SNX improved the antihypertensive effect of nifedipine and apparently improved nifedipine‐induced renal pathological changes, dyslipidemia and the levels of adipogenesis gene expression in kidney tissues. SNX reduced the levels of interleukin‐6 and interleukin‐1β in renal tissues, down‐regulated the production of malondialdehyde, and increased superoxide dismutase activity and the protein expression of heme oxygenase‐1 in kidney tissues. Network pharmacology also showed that SNX could improve nifedipine‐induced renal lipotoxicity. The combination of SNX and nifedipine had certain benefits in the treatment of hypertension.

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Section: Discussionsupporting

confidence: 64%

Shunaoxin pills improve the antihypertensive effect of nifedipine and alleviate its renal lipotoxicity in spontaneous hypertension rats

Liu

Man

Luo

et al. 2020

Environmental Toxicology

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“…A large amount of ROS can destroy cell structure and cell function, and even cause cell death. Xiong et al studied the mechanism of the toxicity of GO on zebrafish, and they found that GO-induced liver injury was mainly mediated by ROS and MAPK signals (Xiong et al, 2020). Pelin et al found that fewlayer-graphene and GO mediated a significant increase in intracellular ROS production primarily through activation of flavin-based oxidase in human HaCaT skin keratinocytes, and caused significant mitochondrial membrane depolarization and mitochondrial damage (Pelin et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Yang

Pan²,

Chen

et al. 2020

Front. Pharmacol.

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Graphene, known as "black gold", has important applications in various fields. In previous studies, it has been proved that graphene oxide (GO) which is a derivative of graphene has low toxicity. However, the immunotoxicity of GO has not been fully elucidated. In this work, we used DC2.4 cell line to investigate the in vitro immunotoxicity of two types of GO, mono-layer GO (mono-GO) and multi-layer GO (multi-GO). We found that mono-GO had less effect on cell viability than multi-GO, but both mono-GO and multi-GO significantly induced the generation of ROS in DC2.4 cells. Interestingly, mono-GO caused DC2.4 cells to aggregate, thus changed the cell morphology significantly. However, no similar influence occurred for multi-GO. In addition, the results showed that these two GOs obviously enhance the release of TNF-a by DC2.4 cells with and without LPS stimulation. GO did not affect the level of IL-6 released from DC2.4 cells, but multi-GO promoted the release of IL-6 while mono-GO inhibited the production of IL-6 when cells were in response to LPS stimulation. Whole-transcriptome sequencing analysis found some immune-related differentially expressed genes including H2-DMb1, Ncbp3, Oas2, Men1, Fas, Cd320, Cd244, and Tinagl1 which are engaged in the immune system process. These results suggested that both mono-GO and multi-GO are immunotoxic to DC2.4 cells, which provides important basis for subsequent biological and clinical medical applications.

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“…Moreover, GO induced NF-κB p65 into the nucleus and increased the protein levels of NF-κB p65, JAK2, STAT3, and Bcl2 in adult zebrafish liver. Overall, the elaborative study and results demonstrated GO-induced hepatic dysfunction mainly through the ROS and PPAR-α-mediated innate immune signaling in zebrafish [98].…”

Section: Exposure Accumulation and Bio-distribution Of Graphene In mentioning

confidence: 77%

“…The chorion has been reported as a major barrier to prevent nanomaterial uptake by fish embryos [114]. In this consideration, microinjection can be used to deliver nanomaterials to the developmental embryos [60,98]. However, in this particular treatment, the injected nanomaterials will be eventually diluted over time with the rapidly dividing embryonic cells.…”

Section: Current Understanding Of Graphene and Graphene Oxide (Go) Tomentioning

confidence: 99%

Toxicity Studies on Graphene-Based Nanomaterials in Aquatic Organisms: Current Understanding

et al. 2020

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Graphene and its oxide are nanomaterials considered currently to be very promising because of their great potential applications in various industries. The exceptional physiochemical properties of graphene, particularly thermal conductivity, electron mobility, high surface area, and mechanical strength, promise development of novel or enhanced technologies in industries. The diverse applications of graphene and graphene oxide (GO) include energy storage, sensors, generators, light processing, electronics, and targeted drug delivery. However, the extensive use and exposure to graphene and GO might pose a great threat to living organisms and ultimately to human health. The toxicity data of graphene and GO is still insufficient to point out its side effects to different living organisms. Their accumulation in the aquatic environment might create complex problems in aquatic food chains and aquatic habitats leading to debilitating health effects in humans. The potential toxic effects of graphene and GO are not fully understood. However, they have been reported to cause agglomeration, long-term persistence, and toxic effects penetrating cell membrane and interacting with cellular components. In this review paper, we have primarily focused on the toxic effects of graphene and GO caused on aquatic invertebrates and fish (cell line and organisms). Here, we aim to point out the current understanding and knowledge gaps of graphene and GO toxicity.

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Graphene oxide nanoparticles induce hepatic dysfunction through the regulation of innate immune signaling in zebrafish (Danio rerio)

Cited by 30 publications

References 56 publications

Shunaoxin pills improve the antihypertensive effect of nifedipine and alleviate its renal lipotoxicity in spontaneous hypertension rats

Shunaoxin pills improve the antihypertensive effect of nifedipine and alleviate its renal lipotoxicity in spontaneous hypertension rats

Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Toxicity Studies on Graphene-Based Nanomaterials in Aquatic Organisms: Current Understanding

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