Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Yang, Zhiwen; Pan, Yongning; Chen, Tingting; Li, Li; Zou, Wenyi; Liu, Dongmeng; Xue, Dahui; Wang, Xiaomei; Lin, Guimiao

doi:10.3389/fphar.2020.01206

Cited by 30 publications

(28 citation statements)

References 45 publications

(60 reference statements)

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“…Therefore, this type of multi-layer GO with more than 10 layers is more toxic than other few-layer GO studied previously in murine NIH-3T3 fibroblasts, U87 and U118 glioma cells, human lung fibroblasts cells, and even human skin HaCaT cells, up to 100 µg/ml [7][8][9][10]13]. These results are in good agreement with other studies such as that in which multi-GO showed more toxicity on cell viability than mono-GO in DC2.4 dendritic cells [11].…”

Section: Cytotoxicity Assaysupporting

confidence: 90%

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Multi-Layer Graphene Oxide in Human Keratinocytes: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Salesa¹,

Serrano‐Aroca²

2021

Preprint

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Few-layer graphene oxide (GO) has shown none or very weak cytotoxicity and anti-proliferative effects in a wide range of cell lines such as glyoma cells and human skin HaCaT cells, in concentrations up to 100 µg/mL However, multi-layer GO has been hardly explored in the biomedical field. Thus, multi-layer GO was examined here in human keratinocyte HaCaT cells treated with different concentrations ranging from 0.01 to 150 µg/mL during different periods of times (3, 12 and 24 hours). The results of this study showed a time-concentration dependence with two non-cytotoxic concentrations (0.01 and 0.05 µg/mL) and a median effective concentration value of 4.087 µg/mL at 24 hours of GO exposure. Contrary to what has been reported for few-layer GO, cell proliferation of the HaCaT cells in contact with the multi-layer GO at 0.01 μg/mL showed identical proliferative activity compared to an epidermal growth factor (1.6-fold greater than the control group) after 96 hours. The effects of the multi-layer GO on the expression of 13 genes (SOD1, CAT, MMP1, TGFB1, GPX1, FN1, HAS2, LAMB1, LUM, CDH1, COL4A1, FBN and VCAN) at the non-cytotoxic concentrations of GO in the HaCaT cells were analyzed after 24 hours. Thus, the lowest non-cytotoxic GO concentration was able to up-regulate the CAT, TGFB1, FN1 and CDH1 genes, which confirms the great potential of multi-layer GO in the biomedical field.

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Section: Cytotoxicity Assaysupporting

confidence: 90%

“…In the same research line, monolayer GO has shown less effect on cell viability than multi-GO in DC2.4 dendritic cells [11]. In that study, it was demonstrated that both mono-GO and multi-GO significantly induced the generation of reactive oxygen species in the DC2.4 cells.…”

Section: Introductionmentioning

confidence: 78%

Multi-Layer Graphene Oxide in Human Keratinocytes: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Salesa¹,

Serrano‐Aroca²

2021

Preprint

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“…Single-layer GO showed low toxicity in NIH-3T3 fibroblast cells [18], and single-or two-layer GO in human lung fibroblast (HLF) cells assessed with methyl thiazolyl tetrazolium showed no cytotoxicity at 10 µg/mL after 24 h [19], nor did a few-layer GO dose of less than 20 µg/mL exhibit toxicity in human fibroblast cells [9]. In the same line of research, monolayer GO has shown a lesser effect than multi-GO on cell viability in DC2.4 dendritic cells [20]. This study showed that both mono-GO and multi-GO significantly induced the generation of reactive oxygen species in the DC2.4 cells.…”

Section: Introductionmentioning

confidence: 88%

Multi-Layer Graphene Oxide in Human Keratinocytes: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Salesa

Serrano‐Aroca

2021

Coatings

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Few-layer graphene oxide (GO) has shown no or very weak cytotoxicity and anti-proliferative effects in a wide range of cell lines, such as glioma cells and human skin HaCaT cells at concentrations up to 100 µg/mL. However, as multi-layer GO has hardly been explored in the biomedical field, in this study, this other type of GO was examined in human keratinocyte HaCaT cells treated with different concentrations, ranging from 0.01 to 150 µg/mL, for different periods of time (3, 12, and 24 h). The results revealed a time–concentration dependence with two non-cytotoxic concentrations (0.01 and 0.05 µg/mL) and a median effective concentration value of 4.087 µg/mL at 24 h GO exposure. Contrary to what has previously been reported for few-layer GO, cell proliferation of the HaCaT cells in contact with the multi-layer GO at 0.01 μg/mL showed identical proliferative activity to an epidermal growth factor (1.6-fold greater than the control group) after 96 h. The effects of the multi-layer GO on the expression of 13 genes (SOD1, CAT, MMP1, TGFB1, GPX1, FN1, HAS2, LAMB1, LUM, CDH1, COL4A1, FBN, and VCAN) at non-cytotoxic concentrations of GO in the HaCaT cells were analyzed after 24 h. The lowest non-cytotoxic GO concentration was able to upregulate the CAT, TGFB1, FN1, and CDH1 genes, which confirms multi-layer GO’s great potential in the biomedical field.

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“…To search the proteins combined with XIST, we performed the starBase database and preliminarily determined 29 candidate proteins. Among them, only 6 ones were co-expressed with XIST, including TNRC6, DGCR8, C17ORF85 (NCBP3), ZC3H7B, SFRS1 and TIA1, all of which were involved in the development and progression of diverse tumors [41][42][43][44][45][46]. Thus, we identified that these six proteins potentially interacted with XIST.…”

Section: Discussionmentioning

confidence: 97%

Prognostic Roles of LncRNA XIST and Its Potential Mechanisms in Human Cancers: A Pan-Cancer Analysis

Wang

Shi

et al. 2021

Preprint

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Background: X-inactive specific transcript (XIST), it has been found, is abnormal expression in various neoplasms. This work aims to explore its potential molecular mechanisms and prognostic roles in types of malignancies. Methods: This research comprehensively investigated XIST transcription across cancers from Oncomine, TIMER 2.0 and GEPIA2. Correlations of XIST expression with prognosis, miRNAs, interacting protens, immune infiltrates, checkpoint markers and mutations of tumor-associated genes were also analyzed by public databases. Results: Compared to normal tissues, XIST was lower in BRCA, COAD, LUAD, lymphoma and OV in Oncomine; In TIMER 2.0, XIST was decreased in BRCA, KICH, THCA and UCEC, but increased in KIRC and PRAD; In GEPIA2, XIST was down-regulated in CESC, COAD, OV, READ, STAD, UCEC and UCS. Public databases also showed that XIST was a good indicator of prognosis in BRCA, CESC, COAD, STAD, OV and so on, but a bad one in KIRC, KIRP and so on. From starBase, we found 29 proteins interacting with XIST, and identified 4 miRNAs, including miR-103a-3p, miR-107, miR-130b-3p and miR-96-5p, which might be sponged by XIST in cancers. Furthermore, XIST was linked with immune infiltration, especially T cell CD4+, and was related to over 20 immune checkpoint markers. In addition, XIST was associated with several tumor-associated gene mutations in some cancers. Conclusion: In summary, abnormal expression of XIST influenced prognosis, miRNAs, immune cell infiltration and mutations of tumor-associated genes across cancers, especially BRCA and colorectal cancer. More efforts should be made to detect potential molecular mechanisms of XIST in the carcinogenesis.

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Cytotoxicity and Immune Dysfunction of Dendritic Cells Caused by Graphene Oxide

Cited by 30 publications

References 45 publications

Multi-Layer Graphene Oxide in Human Keratinocytes: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Multi-Layer Graphene Oxide in Human Keratinocytes: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Multi-Layer Graphene Oxide in Human Keratinocytes: Time-Dependent Cytotoxicity, Proliferation, and Gene Expression

Prognostic Roles of LncRNA XIST and Its Potential Mechanisms in Human Cancers: A Pan-Cancer Analysis

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