Graphene oxide incorporated polystyrene electrospun nanofibers for immunosensing of CD36 as a marker of diabetic plasma

Er, Simge; Demirkol, Dilek Odacı

doi:10.1016/j.bioelechem.2022.108083

Cited by 16 publications

(15 citation statements)

References 54 publications

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“…In the literature studies, it was indicated that DMF is the most suitable solvent to produce smooth and bead-free PS nanofibers due to its high dielectric constant (36.4) [ 41 , 42 , 43 ]. In addition, we optimized all electrospinning parameters to produce PS nanofibers with optimum morphological properties in our previous study [ 44 ]. Hence, DMF was chosen as a solvent for the PS (35%; w/v).…”

Section: Resultsmentioning

confidence: 99%

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Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Ketmen

Gelen

et al. 2022

Biosensors

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The large surface area/volume ratio and controllable surface conformation of electrospun nanofibers (ENFs) make them highly attractive in applications where a large surface area is desired, such as sensors and affinity membranes. In this study, nanocomposite-based ENFs were produced and immobilization of Anti-CRP was carried out for the non-invasive detection of C-reactive protein (CRP). Initially, the synthesis of graphene oxide (GO) was carried out and it was modified with magnetic nanoparticles (MNP, Fe3O4) and polydopamine (PDA). Catechol-containing and quinone-containing functional groups were created on the nanocomposite surface for the immobilization of Anti-CRP. Polystyrene (PS) solution was mixed with rGO-MNP-PDA nanocomposite and PS/rGO-MNP-PDA ENFs were produced with bead-free, smooth, and uniform. The surface of the screen-printed carbon electrode (SPCE) was covered with PS/rGO-MNP-PDA ENFs by using the electrospinning technique under the determined optimum conditions. Next, Anti-CRP immobilization was carried out and the biofunctional surface was created on the PS/rGO-MNP-PDA ENFs coated SPCE. Moreover, PS/rGO-PDA/Anti-CRP and PS/MNP-PDA/Anti-CRP immunosensors were also prepared and the effect of each component in the nanocomposite-based electrospun nanofiber (MNP, rGO) on the sensor response was investigated. The analytic performance of the developed PS/rGO-MNP-PDA/Anti-CRP, PS/rGO-PDA/Anti-CRP, and PS/MNP-PDA/Anti-CRP immunosensors were examined by performing electrochemical measurements in the presence of CRP. The linear detection range of PS/rGO-MNP-PDA/Anti-CRP immunosensor was found to be from 0.5 to 60 ng/mL and the limit of detection (LOD) was calculated as 0.33 ng/mL for CRP. The PS/rGO-MNP-PDA/Anti-CRP immunosensor also exhibited good repeatability with a low coefficient of variation.

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Section: Resultsmentioning

confidence: 99%

“…It was reported in the literature that SAA concentrations are generally between 20 and 50 mg/L for humans [ 71 ]. INS should be lower than 12 µU/mL in blood for healthy persons [ 44 , 72 ]. However, the INS level in saliva is 10 times lower than its blood concentration [ 73 ].…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Ketmen

Gelen

et al. 2022

Biosensors

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“…Especially, label-free immunosensors have attracted a lot of attention since they offer high selectivity, specificity toward the targeted analyte, and low-cost diagnosis of biomolecules related to different diseases. 8 Many nanomaterials such as carbon nanotubes (CNTs), 9 graphene oxide (GO), 10 colloidal nanoparticles, 11 quantum dots, 12 electrospun nanofibers (ENs), 13 and dendrimers 14 between the electrode and the receptor. 15 Additionally, interest in ENs is increasing day by day due to their improved properties such as high adsorption capacity, porous structure, and biocompatibility.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Especially, label-free immunosensors have attracted a lot of attention since they offer high selectivity, specificity toward the targeted analyte, and low-cost diagnosis of biomolecules related to different diseases . Many nanomaterials such as carbon nanotubes (CNTs), graphene oxide (GO), colloidal nanoparticles, quantum dots, electrospun nanofibers (ENs), and dendrimers can be used in sensors. Among them, CNTs can provide direct electron transfer between the electrode and the receptor .…”

Section: Introductionmentioning

confidence: 99%

“…A polystyrene/graphene oxide-(3-aminopropyl)triethoxysilane/Anti-CD36 (PS/GO-APTES/Anti-CD36) immunosensor system was developed for the detection of CD36 using electrochemical measurements in our previous study, which was the first study of a label-free electrochemical immunosensor for CD36 . This study is the second study of the label-free electrochemical immunosensor for CD36 detection in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Carbon Nanotube-Incorporated Nanofibers for Immunosensor Preparation against CD36

Demirkol

2023

ACS Omega

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The increased serum concentration of CD36 is significantly associated with atherosclerosis, insulin resistance, and diabetes mellitus. Currently, there is no sensor system used for the detection of CD36 in the clinical field. Therefore, there is a need to develop a sensor system for the detection of CD36. The large surface area/volume ratio and controllable surface conformation of electrospun nanofibers (ENs) make them highly attractive for immunosensor applications. In the present study, PS/MWCNT-PAMAM ENs were produced and used as an immobilization matrix of Anti-CD36. Thus, the electrochemical behavior of the developed nanocomposite-based ENs and their usage potential were investigated for immunosensor applications. First, an oxidized multiwall carbon nanotube (MWCNT-OH) was synthesized and modified with a polyamidoamine generation 3 (PAMAM G3) dendrimer. The synthesized MWCNT-PAMAM nanocomposite was mixed with polystyrene (PS) solutions at different ratios to produce bead-free, smooth, and uniform PS/MWCNT-PAMAM ENs. PS/MWCNT-PAMAM ENs were accumulated on a screen-printed carbon electrode (SPCE) using the electrospinning technique. A biofunctional surface on the PS/MWCNT-PAMAM EN-coated SPCE was created using carbodiimide chemistry by covalent immobilization of Anti-CD36. The analytic performance characteristics of the developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor were determined by performing electrochemical measurements in the presence of the CD36 protein. The linear detection range was found to be from 5 to 40 ng/mL, and the limit of detection was calculated as 3.94 ng/mL for CD36. The developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor also displayed high tolerance to interference substances, good repeatability, and high recovery percent (recovery%) for artificial blood serum analysis.

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Efficacious Removal of Flonicamid Insecticide from Water by GO@functionalized Calix[4]pyrrole: Synergistic Effect in Adsorption

et al. 2023

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The presence of a highly toxic pyridine carboxamide insecticide, namely flonicamid in water bodies poses a serious risk to both the aquatic ecosystem and human health. Therefore, a graphene oxide (GO) decorated with azo‐functionalized calix[4]pyrrole i. e. GO‐azocalix[4]pyrrole (GACP) was synthesized to study the removal of flonicamid insecticide through adsorption. The nano‐adsorbent was characterized by IR, XPS, TGA, Raman, SEM, and TEM data. The effect of concentration of flonicamid solution, contact time, adsorbent dosage, temperature, and pH was investigated in the batch adsorption process. Experimental data suggested that equilibrium was attained within 40 min and adsorption efficiency was 93.28 %. The synergistic effect of GO and azocalix[4]pyrrole played an important role in the adsorption. The Langmuir isotherm was followed and the maximum adsorption capacity was found to be 11.43 mg/g. The pseudo‐second‐order kinetic data was well obeyed and thermodynamic investigation suggested the feasible and spontaneous nature of flonicamid onto GACP nanocomposite.

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Graphene oxide incorporated polystyrene electrospun nanofibers for immunosensing of CD36 as a marker of diabetic plasma

Cited by 16 publications

References 54 publications

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Carbon Nanotube-Incorporated Nanofibers for Immunosensor Preparation against CD36

Efficacious Removal of Flonicamid Insecticide from Water by GO@functionalized Calix[4]pyrrole: Synergistic Effect in Adsorption

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