Graphene oxide–DNA based sensors

Gao, Li; Lian, Chaoqun; Zhou, Yang; Yan, Lirong; Li, Qin; Zhang, Chunxia; Chen, Liang; Chen, Keping

doi:10.1016/j.bios.2014.03.039

Cited by 181 publications

(99 citation statements)

References 84 publications

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“…Since then, this scheme has been adapted in many different systems for detecting a broad range of analytes. 6,[29][30][31] Being highly negatively charged, normally DNA probes cannot spontaneously enter 4 cells. A cationic delivery vehicle or nanostructured DNA is required for intracellular detection.…”

Section: Introductionmentioning

confidence: 99%

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

et al. 2014

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Fluorescent aptamer probes physisorbed on graphene oxide (GO) have recently emerged as a useful sensing platform. Signal is generated by analyte-induced probe desorption. To address non-specific probe displacement and false positive signal, we herein report a covalently linked aptamer probe for ATP detection. A fluorophore and amino dual-modified aptamer was linked to the carboxyl group on GO with a coupling efficiency of ~50%. The linearity, specificity, stability, and regeneration of the covalent sensor was systematically studied and compared to the physisorbed probe. Both sensors have similar sensitivity, but the covalent one is more resistant to non-specific probe displacement by proteins. The covalent sensor has a dynamic range from 0.125 mM to 2 mM ATP in buffer at room temperature and is resistance to DNase I. Intracellular ATP imaging was demonstrated using the covalent sensor, which generated higher fluorescence signal than the physisorbed sensor. After stimulating the cells with 5 mM Ca 2+ for ATP production, the intracellular signal enhanced by 31.8%. This work highlights the advantages of covalent aptamer sensors using GO as both a quencher and a delivery vehicle for intracellular metabolite detection.3

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Section: Introductionmentioning

confidence: 99%

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

et al. 2014

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“…The enriched functional groups can interact in an ionic, covalent or non-covalent manner, so that in principle they provide the highest extraction efficiency of biomolecules per unit area . In recent years, the functionalized GO has been exploited to fabricate biosensors for drug delivery Zhang et al, 2010a), bioimaging in living cells Sun et al, 2008), the detection of cancer cell (Tao et al, 2013), glucose (Song et al, 2010), DNA Gao et al, 2014), enzyme (Zhang et al, 2010b), protein , peptides (Han et al, 2010), and cellulose and lignin .…”

Section: Introductionmentioning

confidence: 99%

Graphene oxide functionalized long period grating for ultrasensitive label-free immunosensing

Liu

Cai

et al. 2017

Biosensors and Bioelectronics

107

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We explore graphene oxide (GO) nanosheets functionalized dual-peak long period grating (dLPG) based biosensor for ultrasensitive label-free antibody-antigen immunosensing. The GO linking layer provides a remarkable analytical platform for bioaffinity binding interface due to its favorable combination of exceptionally high surface-to-volume ratio and excellent optical and biochemical properties. A new GO deposition technique based on chemical-bonding in conjunction with physical-adsorption was proposed to offer the advantages of a strong bonding between GO and fiber device surface and a homogeneous GO overlay with desirable stability, repeatability and durability. The surface morphology of GO overlay was characterized by Atomic force microscopy, Scanning electron microscope, and Raman spectroscopy. By depositing the GO with a thickness of 49.2 nm, the sensitivity in refractive index (RI) of dLPG was increased to 2538 nm/RIU, 200% that of non-coated dLPG, in low RI region (1.333-1.347) where bioassays and biological events were usually carried out. The IgG was covalently immobilized on GO-dLPG via EDC/NHS heterobifunctional cross-linking chemistry leaving the binding sites free for target analyte recognition. The performance of immunosensing was evaluated by monitoring the kinetic bioaffinity binding between IgG and specific anti-IgG in real-time. The GO-dLPG based biosensor demonstrates an ultrahigh sensitivity with limit of detection of 7 ng/mL, which is 10-fold better than non-coated dLPG biosensor and 100-fold greater than LPG-based immunosensor. Moreover, the reusability of GO-dLPG biosensor has been facilitated by a simple regeneration procedure based on stripping off bound anti-IgG treatment. The proposed ultrasensitive biosensor can be further adapted as biophotonic platform opening up the potential for food safety, environmental monitoring, clinical diagnostics and medical applications.

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“…Biosensors are usually comprised by a biological recognition element and a transducer. 1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies. 7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.…”

Section: Introductionmentioning

confidence: 99%

Electro-Immuno Sensors: Current Developments and Future Trends

Barbosa¹,

Segura²,

Osma³

2017

IJBSBE

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Abbreviations: Iot, internet of things; ELISA, enzyme-linked immuno sorbent assay; WB, western blot; IHC, immunohistochemistry; ICC, immunocytochemistry; FACS, fluorescence-activated cell sorting; IP, immuno precipitation; ELISPOT, enzyme-linked immuno spot; PSA, prostate specific antigen; IDEs, interdigitated electrodes; HPV, human papilloma virus IntroductionBiosensors have been mostly used in the food industry, medical and biological fields, among others, where physical and chemical sensors cannot accurately measure all variables. Biosensors are usually comprised by a biological recognition element and a transducer.1,2 Among the different commercial biosensors, the most common recognition elements are DNA, 3 enzymes, 4 aptamers 5,6 and antibodies.7,8 DNA-based biosensors detect specific analytes by their cleavage and ligation to a functionalized DNA strand.9 Enzymatic biosensors immobilize an enzyme to an electrode to facilitate the transport of electrons from the enzyme to the electrode, produced by specific reactions at the active site of the enzyme.2 Aptamers are chemically synthesized oligonucleotides 10 that provide high specificity and sensitivity towards a specific target. Antibody-based biosensors recognize a specific antigen from the immobilization of a monoclonal or polyclonal antibody.8 In general, transducers vary according to the end user towards which the sensor is thought. Among others, transducers include electrochemical responses, mass changes, optical absorption or transmission, and thermal readings. During the last decades, several immunoassay techniques have appeared as a response to the need of having specific and sensitive tests for antigen recognition. The methods by which the recognition is perform varies from one to another, but the presence of at least one recognition antibody remains as a constant. Among others, the most common immune tests used in laboratories and medical facilities are the enzyme-linked immuno sorbent assay (ELISA), western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), fluorescence-activated cell sorting (FACS), immuno precipitation (IP), and enzyme-linked immuno spot (ELISPOT). Figure 1 shows a general diagram of these immunoassays.ELISA is a widely used technique for the recognition of antibodies' antigens within a sample by means of the biochemical recognition of an enzyme.11 In this technique, specific antibodies are conjugated to an enzyme that catalyzes a colorimetric molecule. Spectrophotometric measurements are used to determine the antigen concentration in the sample. ELISA tests can be performed in a qualitative way, in which the results are positive or negative by statistical methods; or quantitative, in which a standard curve from spectrophotometric measurements can be used to quantify the concentration of the antigen. 12WB is a technique for the detection of specific proteins in a sample through the electrophoretic transfer from a separating gel to a blotting matrix.13 WB uses available monoclonal or polyclonal antibodies for the d...

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Graphene oxide–DNA based sensors

Cited by 181 publications

References 84 publications

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Intracellular Detection of ATP Using an Aptamer Beacon Covalently Linked to Graphene Oxide Resisting Nonspecific Probe Displacement

Graphene oxide functionalized long period grating for ultrasensitive label-free immunosensing

Electro-Immuno Sensors: Current Developments and Future Trends

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