Graphene Oxide Conjugated Magnetic Beads for RNA Extraction

Pham, Xuan‐Hung; Baek, Ahruem; Kim, Tae Han; Rho, Won‐Yeop; Chung, Woo‐Jae; Kim, Dong-Eun; Jun, Bong‐Hyun

doi:10.1002/asia.201700554

Cited by 19 publications

(13 citation statements)

References 42 publications

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“…For application, GO has been shown to be a suitable platform for protein and nucleic acid adsorption [14,61]. However, the separation of GO from solution is a time-consuming and complicated process [14]. As mentioned above, the MNPs@SiO 2 was easily collected when conjugated on the surface of GO in our study, Therefore, we used the GO/MNPs@SiO 2 nanocomposites with superparamagnetic property as an isolation material to investigate the isolation of protein in solution.…”

Section: The Magnetization and Protein Isolation Of Silica-coated Magmentioning

confidence: 88%

“…Narrow intense peaks at 1715 cm −1 and 1621 cm −1 represent characteristic stretching of -COO-groups and C=C vibration of aromatic chains on the GO surface. GO also showed peaks at 1352, 1216, 1036, and 974 cm −1 representing the carboxyl/carbonyl, epoxy, and alkoxy groups, respectively [14,[56][57][58].…”

Section: Preparation Of Graphene Oxide Grafted Polyethyleneimine (Go-mentioning

confidence: 99%

“…In conclusion, this result suggests that the GO/MNP@SiO2 nanostructure can be used for protein and nucleic isolation. For application, GO has been shown to be a suitable platform for protein and nucleic acid adsorption [14,61]. However, the separation of GO from solution is a time-consuming and complicated process [14].…”

Section: The Magnetization and Protein Isolation Of Silica-coated Magmentioning

confidence: 99%

“…This result indicates that BSA can be easily adsorbed onto the GO/MNP@SiO 2 nanocomposite and collected due to the presence of GO as a platform and MNP as a magnetic separator material. In our previous report, we used graphene oxide conjugated magnetic beads to extract total RNA and miRNA from non-small cell lung cancer cells [14]. Therefore, we believed that it is also possible to isolate RNA and miRNA from a matrix containing sugars, DNA and other biomolecules by using GO/MNP@SiO 2 nanomaterial.…”

Section: The Magnetization and Protein Isolation Of Silica-coated Magmentioning

confidence: 99%

“…As a result, graphene oxide is utilized in many applications, such as catalysis [2], sensing [3], energy [4], drug delivery [5], and biomedical applications-deep brain stimulators [6], blood glucose sensors [7], tissue engineering [8,9], gene therapy [10], cell imaging [11], cancer therapies [12], and to differentiate and image stem cells [13]. However, the separation of GO from solution is a time-consuming and complicated process [14].…”

Section: Introductionmentioning

confidence: 99%

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Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation

et al. 2020

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In this study, silica-coated magnetic iron oxide nanoparticles (MNPs@SiO2) were covalently conjugated onto graphene oxide (GO/MNP@SiO2) for protein isolation. First, MNPs were precisely coated with a silica layer on the surface by using the reverse microemulsion method, followed by incubation with 3-glycidyloxypropyltrimethoxysilane (GPTS) to produce the GPTS-functionalized MNPs@SiO2 (GPTS-coated MNPs@SiO2) that display epoxy groups on the surface. The silica shell on the MNPs was optimized at 300 µL of Igepal®CO-520, 5 mg of MNP, 100 µL of TEOS, 100 µL of NH4OH and 3% of 3-glycidyloxypropyltrimethoxysilane (GPTS). Simultaneously, polyethyleneimine (PEI) was covalently conjugated to GO to enhance the stability of GO in aqueous solutions and create the reaction sites with epoxy groups on the surface of GPTS-coated MNP@SiO2. The ratio of PEI grafted GO and GPTS-coated MNP@SiO2 (GO/MNP ratio) was investigated to produce GO/MNPs@SiO2 with highly saturated magnetization without aggregation. As a result, the GO/MNP ratio of 5 was the best condition to produce the GO/MNP@SiO2 with 9.53 emu/g of saturation superparamagnetization at a magnetic field of 2.0 (T). Finally, the GO/MNPs@SiO2 were used to separate bovine serum albumin (BSA) to investigate its protein isolation ability. The quantity of BSA adsorbed onto 1 mg of GO/MNP@SiO2 increased sharply over time to reach 628 ± 9.3 µg/mg after 15 min, which was 3.5-fold-higher than that of GPTS-coated MNP@SiO2. This result suggests that the GO/MNP@SiO2 nanostructure can be used for protein isolation.

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Section: The Magnetization and Protein Isolation Of Silica-coated Magmentioning

confidence: 88%

Section: Preparation Of Graphene Oxide Grafted Polyethyleneimine (Go-mentioning

confidence: 99%

Section: The Magnetization and Protein Isolation Of Silica-coated Magmentioning

confidence: 99%

Section: The Magnetization and Protein Isolation Of Silica-coated Magmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation

et al. 2020

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Solid‐phase extraction methods for nucleic acid separation. A review

Yue

et al. 2021

J of Separation Science

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The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid‐phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid‐phase extraction. Herein, solid‐phase extraction methods are systematically reviewed to outline research progress and explore additional solid‐phase sorbents and devices for novel, flexible, and high‐efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic‐free solid‐phase extraction methods, separation device design and optimization, and high‐throughput automatable applications based on high‐performance solid‐phase extraction are summarized. Finally, the current challenges and promising topics are discussed.

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Protein Binding on the Surface of Magnetic Nanoparticles

Sun

Shi

et al. 2019

Part & Part Syst Charact

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Magnetic nanoparticles (MNPs) are widely used in the areas of biology and biomedicine. The interaction between MNPs and proteins plays a crucial role in the bioapplication of MNPs, and the binding affinity of protein–MNPs is the manifestation of this interaction. The binding affinity of some proteins with MNPs modified in various ways is determined by fluorescence quenching. The results show that the binding affinity depends on the properties of both the MNPs and the proteins. The higher the surface curvature of MNPs, the larger the MNP, and the higher the binding affinity. No significant difference is found in binding affinity between MNPs with different modification methods. For proteins, the binding affinity depends on the properties of individual proteins, such as the amino acid sequence, the native protein conformation in solution, the isoelectric point, and surface potential. In general, the binding affinity is higher for proteins with cysteine residues on the surface. In addition, pH affects the binding affinity between proteins and MNPs; positively charged proteins and lower pH are more suitable for MNP binding due to electrostatic forces.

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Graphene Oxide Conjugated Magnetic Beads for RNA Extraction

Cited by 19 publications

References 42 publications

Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation

Silica-Coated Magnetic Iron Oxide Nanoparticles Grafted onto Graphene Oxide for Protein Isolation

Solid‐phase extraction methods for nucleic acid separation. A review

Protein Binding on the Surface of Magnetic Nanoparticles

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