Graphene oxide-chitosan nanocomposite based electrochemical DNA biosensor for detection of typhoid

Thiol Modified Chitosan Self-Assembled Monolayer Platform for Nucleic Acid Biosensor

“…3b). The higher Г for SH-CHIT/Au electrode indicates that large number of redox moieties are available for oxidation leading to higher faradic current wherein, the presence of delocalized electrons due to thiolation, results in increased electron transport between redox species and electrode [20][21][22][23][24]. The electrochemical response of ssDNA-SH-CHIT/Au bioelectrode after hybridization with 0.01-100 μM of complementary target DNA (dsDNA) has been studied by differential pulse voltammetry (DPV) technique in PBS (50 mM, pH 7.0, 0.9 % NaCl) using methylene blue (MB) as redox indicator.…”

A Rapid and Sensitive Diagnosis of Typhoid Fever Based On Nested PCR-Voltammetric DNA Biosensor Using Flagellin Gene Fragment

“…The most redox active among the four nucleic acids bases is the guanine moiety [10][11]. However, few works have been done to detect the S. typhi bacteria via Vi gene using gold nanoparticles (AuNPs) modified SPE for DNA hybridization assay using methylene blue as redox hybridization indicator [12], graphene oxide (GO)-Chitosan (CHI) nano-composite [13], direct electrodeposition on gold nano-aggregates (GNAs) [8], and the amino modified salmonella ssDNA probe sequence was covalently linked with carboxylic group on the surface of nanoporous glassy carbon electrode to prepare the DNA biosensor [9]. The other detection methods of S. typhi and S. typhimurium generally focus on immunological methods and polymerase chain reactions [4][5][14][15][16][17][18].…”

Electrochemical DNA biosensor based on poly(2,6-pyridinedicarboxylic acid) modified glassy carbon electrode for the determination of anticancer drug gemcitabine

“…In recent years, considerable interest has been devoted to DNA biosensors to develop new techniques for the detection of many important molecules such as drugs [7][8][9] pathogens [10], and organic dyes [11,12]. Electrochemical DNA biosensors are constructed by immobilizing DNA on the electrode surface [13,14].…”