Thiol Modified Chitosan Self-Assembled Monolayer Platform for Nucleic Acid Biosensor

Mukherjee, Maumita Das; Solanki, Pratima R.; Sumana, Gajjala; Manaka, Takaaki; Iwamoto, Mitsumasa; Malhotra, Bansi D.

doi:10.1007/s12010-014-1177-7

Cited by 9 publications

(4 citation statements)

References 25 publications

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“…Several other special molecules might be introduced through the linker when preparing the carbohydrate SAM that can be easily seen through the change in position and sudden appearance or disappearance of the bands. Amide group present in the linker of the carbohydrate SAM was found to show bands at ≈1650 and ≈1560 cm −1 for amide I and amide II, respectively [34,88]. Similarly, if the hydroxyl groups are protected with an acetyl group, a strong band will be seen at 1765 cm −1 due to ester functionalities.…”

Section: Chemical Compositionmentioning

confidence: 95%

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Bhattarai¹,

Neupane²,

Mikhaylov³

et al. 2017

Carbohydrate

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Self-assembled monolayers (SAMs) presenting carbohydrates (glycans) have been widely prepared on gold surfaces to mimic the carbohydrate surfaces that are involved in molecular recognition phenomena in living cells. The binding affinity of carbohydrate immbolized on SAM surfaces to various carbohydrate-binding proteins (such as lectins) can be studied by optical, electrochemical, piezoelectrical and thermal sensing techniques. The lectins present on the surface of pathogens (e.g., bacteria or viruses) can be used as targets for capturing onto carbohydrates immobilized on SAM surfaces. The immobilized carbohydrates can also be used for detecting different types of disease biomarkers present in bodily fluids. Synergistic properties of carbohydrate SAMs and gold nanoparticles can be used for vaccine preparation and drug delivery. By studying different types of glycans, their properties, and the behavior toward recognition of specific pathogens and biomarkers, we can develop not only new therapeutics but also enhance the diagnostic strategies of various diseases. In this chapter, we discuss carbohydrate-terminated SAMs and their common preparation strategies. Next, we focus on roles of different components of SAMs, characterization techniques, and applications.

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Section: Chemical Compositionmentioning

confidence: 95%

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Bhattarai¹,

Neupane²,

Mikhaylov³

et al. 2017

Carbohydrate

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“…Along with the membrane-forming ability, TMC makes more space with a cationic charge, which helps in the attachment of the Au nanoparticle with the TMC/FeSO4 nanoparticle and can be a reason for increased response in electrochemical sensing . A self-assembled monolayer of thiolated chitosan on gold coated glass plate can also be fabricated to act as a nucleic acid biosensor due to its delocalized electrons . An electrical biosensor made up with N -CMC combined with molybdenum carbide nanocomposite can be applied in the detection of microRNA-21 …”

Section: Biomedical Applications Of Modified Chitosanmentioning

confidence: 99%

“…239 A self-assembled monolayer of thiolated chitosan on gold coated glass plate can also be fabricated to act as a nucleic acid biosensor due to its delocalized electrons. 240 An electrical biosensor made up with N-CMC combined with molybdenum carbide nanocomposite can be applied in the detection of microRNA-21. 241…”

Section: Antimicrobial Applicationsmentioning

confidence: 99%

Chitosan Derivatives as Carriers for Drug Delivery and Biomedical Applications

Manna

Seth

Gupta

et al. 2023

ACS Biomater. Sci. Eng.

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Over the past few decades, chitosan (CS) has gained the attention of researchers investigating newer biomaterial-based carriers for drugs in pharmaceutical and biomedical research. Combined with its nontoxic behavior, biodegradability, and biocompatibility, chitosan has found widespread applications in the fields of drug delivery, tissue engineering, and cosmetics. As a novel drug carrier, chitosan is regarded as one of the promising biomaterials in the pharmaceutical industry. The extensive use of this cationic biopolysaccharide in the delivery of therapeutic agents has brought a few limitations of chitosan into the limelight. Various chemical modifications of chitosan can minimize these limitations and improve the efficacy of chitosan as a drug carrier. The effectiveness of several chemically modified chitosan derivatives, including trimethyl chitosan, thiolated chitosan, PEGylated chitosan, and other chitosan derivatives, has been investigated by many researchers for the controlled and target specific delivery of therapeutics. The chemically modified chitosan derivatives exhibited greater importance in the current scenario on drug delivery due to their solubility in wide range of media along with their interaction with pharmaceutically active ingredients. Chitosan derivatives have also attracted attention in several biomedical fields, including wound healing, hyperthermia therapy, tissue engineering, and bioadhesives. The present review narrates the sources and common physicochemical properties of chitosan, including several important synthetic modifications to obtain chemically modified chitosans and their applications in targetspecific drug delivery, along with several biomedical applications.

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“…In order to improve detection methods of LM, biosensor technology has been developed 38 – 40 . This approach is based on the interactions between the bacteria and bio-receptors immobilized on different solid-state supports 41 .…”

Section: Introductionmentioning

confidence: 99%

A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces

Capo

D’Auria

Lacroix³

2020

Sci Rep

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Listeria monocytogenes is a foodborne pathogen responsible for human listeriosis. The increasing incidence of listeriosis induced governments and food manufacturing enterprises to act to diminish the problem. Several methods for the detection of Listeria monocytogenes in food industries were developed. However, they are time-consuming and require the use of specialized equipment. To reduce the detection time of Listeria monocytogenes in food, in this work we developed a fluorescence sandwich immunoassay based on the use of an innovative chitosan-cellulose nanocrystal (CNC) membrane that improves the antigen capture during bacterial growth. The combined use of CNC film for the capture of p60 protein-specific antigen together with the use of fluorescence detection reduced the time of analysis from 24 to 12 h with a limit of detection (LOD) of the assay of 102 CFU/mL (2 Log). In addition, the use of monoclonal anti-PepD covalently immobilized to a CNC membrane assured a high specificity of the assay. Interestingly, the obtained results show no cross-reactivity with the five most diffused pathogen bacteria strains tested.

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Thiol Modified Chitosan Self-Assembled Monolayer Platform for Nucleic Acid Biosensor

Cited by 9 publications

References 25 publications

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Self-Assembled Monolayers of Carbohydrate Derivatives on Gold Surfaces

Chitosan Derivatives as Carriers for Drug Delivery and Biomedical Applications

A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces

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