Graphene oxide-based Fe₃O₄ nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase

Abstract

A chloropropyl-functionalized graphene oxide decorated with Fe3O4 nanoparticles, CPS/GO-Fe3O4@MCM-41, was made, and porcine pancreas lipase (PPL) was immobilized onto the graphene oxide based magnetic nanoparticles via covalent bonding.

Preparation of Carriers Based on ZnO Nanoparticles Decorated on Graphene Oxide (GO) Nanosheets for Efficient Immobilization of Lipase from Candida rugosa

“…Graphene oxide (GO) was prepared using a modified Hummer method [ 22 ]. Graphite flakes (0.6 g), and NaNO 3 (3.0 g) were mixed with an ice-water bath, then H 2 SO 4 (120 mL) and KMnO 4 (16.0 g) was added successively and the mixture stirred slowly for 2 h. After the reaction, the flask was placed in a thermostatted water bath, and stirred at 35 °C for 30 min.…”

“…So far, very few reports about GO-based supports for lipase immobilization have been published. For instance, Jing and co-workers [ 22 ] synthesized a chloropropyl-functionalized graphene oxide decorated with Fe 3 O 4 nanoparticles for the immobilization of porcine pancreatic lipase (PPL); Mohammadi and co-workers [ 23 ] used an isocyanide-based four-component reaction tobimmobilize Rhizomucor miehei lipase (RML) on carboxylated multi-wall carbon nanotubes and carboxylated graphene nanosheets; Li and co-workers [ 24 ] fabricated three dimensional (3D) magnetic graphene oxide-chitosan (GO/CS) composites with orderly self-assembled magnetite (Fe 3 O 4 ) nanoparticles (GO/CS/Fe 3 O 4 ) to was immobilize Candida rugosa lipase (CRL).…”

N-doped graphene/CoFe2O4 catalysts for the selective catalytic reduction of NOx by NH3

“…Graphene oxide was prepared using the Hummers' method. 20 The preparation process was as follows: 5 g of graphite powder, 2.5 g of sodium nitrate and 115 ml of concentrated sulfuric acid were rstly added to a three-necked ask placed in an ice water bath for stirring, and aer being uniformly mixed, 15 g of potassium permanganate was added. The mixture was kept in the ice-water bath for 2 hours, and then transferred from the ask into the thermostat water bath again, and stirred for 30 min at 35 C. Transfer the mixture in the three-necked ask into an ice water bath and then add 230 ml of deionized water.…”

Magnetic Fe3O4@MCM-41 core–shell nanoparticles functionalized with thiol silane for efficient l-asparaginase immobilization

“…Bovine serum albumin was used as standard. In addition, IE of L-ASNase was calculated by measuring the difference between amount of protein loaded and amount of protein in supernatant after immobilization [34]. To determine activities of free and immobilized L-ASNase, Nesslerization method was used as described by Mashburn and Wriston [35].…”