Magnetic Fe₃O₄@MCM-41 core–shell nanoparticles functionalized with thiol silane for efficient l-asparaginase immobilization

Abstract

l-Asparaginase (l-ASNase) is a vital enzyme for medical treatment and food industry. Here, we assessed the use of FeO@Mobil Composition of Matter No. 41 (MCM-41) magnetic nanoparticles as carrier matrix for l-ASNase immobilization. In addition, surface of FeO@MCM-41 magnetic nanoparticles was functionalized with 3-mercaptopropyltrimethoxysilane (MPTMS) to enhance stability of l-ASNase. The chemical structure, thermal properties, magnetic profile and morphology of the thiol-functionalized FeO@MCM-41 magnetic nanoparticles were characterized with Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), differential thermal analysis (DTA), differential scanning calorimetry (DSC), vibrating sample magnetometer (VSM), scanning electron microscope (SEM), energy dispersive X-ray (EDX) spectroscopy and zeta-potential measurement. l-ASNase was covalently immobilized onto the thiol-functionalized FeO@MCM-41 magnetic nanoparticles. The properties of the immobilized enzyme, including optimum pH, temperature, kinetic parameters, thermal stability, reusability and storage stability were investigated and compared to free one. Immobilized enzyme was found to be stable over a wide range of pH and temperature range than free enzyme. The immobilized l-ASNase also showed higher thermal stability after 180 min incubation at 50 °C. The immobilized enzyme still retained 63% of its original activity after 16 times of reuse. The Km value for the immobilized enzyme was 1.15-fold lower than the free enzyme, which indicates increased affinity for the substrate. Additionally, the immobilized enzyme was active over 65% and 53% after 30 days of storage at 4 °C and room temperature (∼25 °C), respectively. Thereby, the results confirmed that thiol-functionalized FeO@MCM-41 magnetic nanoparticles had high efficiency for l-ASNase immobilization and improved stability of L-ASNase.

Magnetic Mesoporous Silica Composite for Enhanced Preconcentration of Selected Organophosphorus Pesticides in Fruits

“…3(b)) indicated the Fe-O stretching [45][46]. The Fe3O4-MCM-41 particles show characteristics peaks at 464 and 1636 cm -1 , which slightly decreased with the appearance of the Fe-O-Si covalent bonding due to OH condensation [44]. Consequently, changes of some characteristic peaks confirmed the Fe3O4 nanoparticles were successfully anchored on the surface of MCM-41.…”

Encapsulation of Asparaginase as a Promising Strategy to Improve In Vivo Drug Performance

“…In addition, immobilized ASNase gained pH and thermal stability, kinetic, reusability and storage stability in comparison with the free enzyme. However, no in vivo assays were performed in this study [74].…”

ANALISA SIFAT LISTRIK DAN KANDUNGAN Fe NANO PARTIKEL PASIR BESI BERBASIS PASIR BESI SUNGAI UNTUK MENGETAHUI KUALITAS AIR SUNGAI

“…Nano partikel Fe3O4 magnetik adalah bahan super paramagnetik,dan memiliki koersivitas tinggi sebagai adsorben (Setyaningsih et al, 2019). Nano partikel magnetik adalah bahan yang memiliki sifat magnetik yang kuat,ukurannya yang kecil, area permukaan yang tinggi, sintesis berbiaya rendah, penyerapan yang tinggi dan biokompatibilitas (Ulu et al, 2018). Bahan berbasis Fe3O4 sangat efektif sebagai adsorben ion logam dalam air, limbah, atau bubur karena dapat dipisahkan dari matriks dengan cepat menggunakan medan magnet eksternal.…”