Graphene Oxide as a Pathogen‐Revealing Agent: Sensing with a Digital‐Like Response

Morales‐Narváez, Eden; Hassan, Abdelrahim H. A.; Merkoçi, Arben

doi:10.1002/anie.201307740

Cited by 57 publications

(38 citation statements)

References 51 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 86 ] This novel glass substrate is also proven to be amenable for optical biosensing applications in the microarray format, showing comparable performances with the standard aminosilane substrate in a sandwich immunoassay and low background fl uorescence upon laser excitation at 533 and 635 nm. Moreover, interfacing nanomaterials and proteins on this new nanoenabled support could provide advanced bioanalytical tools, [ 50 ] and its use could be expanded to the study of nanomaterial-cell interactions. [1][2][3]30,54,87,88 ] The proposed glass nano biofunctionalization is easily scalable through the latest reported techniques, [ 56 ] quick, and environmental friendly allowing a cost-effective modifi cation.…”

Section: Discussionmentioning

confidence: 99%

“…[ 28,46 ] QDs being vigorous donors and GO sheets being powerful energy transfer acceptors, the interplay of these two complementary nanomaterials in rationally designed biological assemblies can open the avenue to unparalleled performances in sensing creating opportunities for the development of low-cost wileyonlinelibrary.com © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim and rapid molecular diagnostics tools, [47][48][49] possibly reaching digital-like responses. [ 50,51 ] Additionally, new dimensions for the elaboration of novel (bio)sensors based on diverse mechanisms of transduction can be created through immobilization of the molecular species on chips with high spatial resolution, using GO as a donor [ 52 ] or an acceptor [ 53 ] of FRET, QDs, [ 54 ] , or a combination of them. [ 50 ] Immobilization is a crucial step to design labs-on-a-surface for multiplex-screening, high-throughput sensing, real-time detection, device miniaturization, and reduction of reagent amount.…”

Section: Introductionmentioning

confidence: 99%

“…[ 50,51 ] Additionally, new dimensions for the elaboration of novel (bio)sensors based on diverse mechanisms of transduction can be created through immobilization of the molecular species on chips with high spatial resolution, using GO as a donor [ 52 ] or an acceptor [ 53 ] of FRET, QDs, [ 54 ] , or a combination of them. [ 50 ] Immobilization is a crucial step to design labs-on-a-surface for multiplex-screening, high-throughput sensing, real-time detection, device miniaturization, and reduction of reagent amount. [ 55,56 ] The disposition of concentrated sensing molecular assemblies in submicrometric sectors, light stimulated by focalized radiation, also allows the signal-to-noise ratio and sensitivity to bump up, therefore reaching very low detection limits.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

On‐the‐Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

Gravagnuolo

Morales‐Narváez

Matos

et al. 2015

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

Class I hydrophobin Vmh2, a peculiar surface active and versatile fungal\ud protein, is known to self-assemble into chemically stable amphiphilic fi lms,\ud to be able to change wettability of surfaces, and to strongly adsorb other\ud proteins. Herein, a fast, highly homogeneous and effi cient glass functionalization\ud by spontaneous self-assembling of Vmh2 at liquid–solid interfaces is\ud achieved (in 2 min). The Vmh2-coated glass slides are proven to immobilize\ud not only proteins but also nanomaterials such as graphene oxide (GO) and\ud quantum dots (QDs). As models, bovine serum albumin labeled with Alexa\ud 555 fl uorophore, anti-immunoglobulin G antibodies, and cadmium telluride\ud QDs are patterned in a microarray fashion in order to demonstrate functionality,\ud reproducibility, and versatility of the proposed substrate. Additionally, a\ud GO layer is effectively and homogeneously self-assembled onto the studied\ud functionalized surface. This approach offers a quick and simple alternative to\ud immobilize nanomaterials and proteins, which is appealing for new bioanalytical\ud and nanobioenabled applications

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

On‐the‐Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

Gravagnuolo

Morales‐Narváez

Matos

et al. 2015

Adv Funct Materials

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, this approach can be extended to DNA, peptide, and protein detection according to the utilized biorecognition probe, see Figure 4B. This biosensing mechanism employs a single type of antibody and has been successfully employed for pathogen detection via several formats based on the solid phase; including microarray technology, [82] lateral flow strips (see Figure 4C), [83] and paper-based devices. Mater.…”

Section: The Solid Phase Opens Up New Opportunities In Go-based Biosementioning

confidence: 99%

Graphene Oxide as an Optical Biosensing Platform: A Progress Report

2018

Self Cite

View full text Add to dashboard Cite

IntroductionAs a 2D material, graphene oxide (GO) is a lattice-like nanostructure of hexagonal carbon rings disrupted by oxygen-containing moieties. In 2012, we discussed the outstanding physicochemical properties offered by GO and how these features can be exploited in unprecedented optical biosensing systems. [1] In fact, GO can be processed in suspension, easily complexed with biomolecules, and offers a universal highly efficient long-range photoluminescence quenching agent-among other functional properties. Furthermore, we highlighted that GO photoluminescences with energy transfer donor/acceptor molecules exposed A few years ago, crucial graphene oxide (GO) features such as the carbon/oxygen ratio, number of layers, and lateral size were scarcely investigated and, thus, their impact on the overall optical biosensing performance was almost unknown. Nowadays valuable insights about these features are well documented in the literature, whereas others remain controversial. Moreover, most of the biosensing systems based on GO were amenable to operating as colloidal suspensions. Currently, the literature reports conceptually new approaches obviating the need of GO colloidal suspensions, enabling the integration of GO onto a solid phase and leading to their application in new biosensing devices. Furthermore, most GO-based biosensing devices exploit photoluminescent signals. However, further progress is also achieved in powerful label-free optical techniques exploiting GO in biosensing, particularly using optical fibers, surface plasmon resonance, and surface enhanced Raman scattering. Herein, a critical overview on these topics is offered, highlighting the key role of the physicochemical properties of GO. New challenges and opportunities in this exciting field are also highlighted.The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/adma.201805043.in a planar surface. [1] However, GO properties can be tailored according to its oxidation degree, lateral size, and number of layers, which was something little explored 6 years ago, and thus their impact on the overall biosensing performance was almost unknown. In addition, most of the biosensing systems based on GO were amenable to working as colloidal suspensions. Though, recent literature reports conceptually new approaches obviating the need of colloidal suspensions, which facilitates integration of GO-based biosensors into the solid phase and allows for their applications in new devices. Furthermore, the majority of the GO-based optical biosensing techniques rely on photoluminescent signals. However, further progress has also been achieved in powerful label-free optical techniques exploiting GO in biosensing. Here, we introduce a progress report on this exciting topic, underscoring challenges and opportunities in (bio)sensing accordingly. Number of LayersGO aqueous suspensions are highly stable in the form of mono layers. However, GO multilayer films can be built via

show abstract

“…This principle could even be applied for a multiplexed colorimetric DNA sensor [107]. Weak interactions with graphene oxide can also be obtained with antibodies labeled with QDs, which were exploited for the detection of the model pathogen E. coli [108]. The fluorescence of a corresponding antibody-modified QD is quenched in the presence of graphene oxide and recovered after the recognition event with the bacteria.…”

Section: Carbon Nanomaterialsmentioning

confidence: 99%

Synergetic Effects of Combined Nanomaterials for Biosensing Applications

Holzinger

Goff

Cosnier

2017

Sensors

View full text Add to dashboard Cite

Nanomaterials have become essential components for the development of biosensors since such nanosized compounds were shown to clearly increase the analytical performance. The improvements are mainly related to an increased surface area, thus providing an enhanced accessibility for the analyte, the compound to be detected, to the receptor unit, the sensing element. Nanomaterials can also add value to biosensor devices due to their intrinsic physical or chemical properties and can even act as transducers for the signal capture. Among the vast amount of examples where nanomaterials demonstrate their superiority to bulk materials, the combination of different nano-objects with different characteristics can create phenomena which contribute to new or improved signal capture setups. These phenomena and their utility in biosensor devices are summarized in a non-exhaustive way where the principles behind these synergetic effects are emphasized.

show abstract

Graphene Oxide as a Pathogen‐Revealing Agent: Sensing with a Digital‐Like Response

Cited by 57 publications

References 51 publications

On‐the‐Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

On‐the‐Spot Immobilization of Quantum Dots, Graphene Oxide, and Proteins via Hydrophobins

Graphene Oxide as an Optical Biosensing Platform: A Progress Report

Synergetic Effects of Combined Nanomaterials for Biosensing Applications

Contact Info

Product

Resources

About