Graphene nanoparticles induces apoptosis in MCF-7 cells through mitochondrial damage and NF-KB pathway

Alsaedi, Iman I J; Taqi, Zainab J.; Hussien, Adi M. Abdul; Sulaiman, Ghassan M.; Jabir, Majid S.

doi:10.1088/2053-1591/ab33af

Cited by 48 publications

(16 citation statements)

References 44 publications

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“…was tested by applying double staining with acridine orange and propidium iodide 24 . Briefly, the cells were placed in 96-well plates, followed by incubation with the IC 50 concentration of Hsp, AuNPs and Hsp-AuNPs (16 h).…”

Section: Acridine Orange and Propidium Iodide Staining The Hsp-aunpsmentioning

confidence: 99%

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Sulaiman

Waheeb

Jabir

et al. 2020

Sci Rep

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185

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Hesperidin is a flavonoid glycoside with proven therapeutic activities for various diseases, including cancer. However, its poor solubility and bioavailability render it only slightly absorbed, requiring a delivery system to reach its therapeutic target. Hesperidin loaded on gold nanoparticles (Hsp-AuNPs) was prepared by a chemical synthesis method. Various characterization techniques such as UV-VIS spectroscopy, ftiR, XRD, feSeM, teM and eDX, Zeta potential analysis, particle size analysis, were used to confirm the synthesis of Hsp-AuNPs. The cytotoxic effect of Hsp-AuNPs on human breast cancer cell line (MDA-MB-231) was assessed using MTT and crystal violet assays. The results revealed significant decrease in proliferation and inhibition of growth of the treated cells when compared with human normal breast epithelial cell line (HBL-100). Determination of apoptosis by fluorescence microscope was also performed using acridine orange-propidium iodide dual staining assay. The in vivo study was designed to evaluate the toxicity of Hsp-AuNPs in mice. The levels of hepatic and kidney functionality markers were assessed. No significant statistical differences were found for the tested indicators. Histological images of liver, spleen, lung and kidney showed no apparent damages and histopathological abnormalities after treatment with Hsp-AuNPs. Hsp-AuNPs ameliorated the functional activity of macrophages against Ehrlich ascites tumor cells-bearing mice. The production of the pro-inflammatory cytokines was also assessed in bone marrow-derived macrophage cells treated with Hsp-AuNPs. The results obviously demonstrated that Hsp-AuNPs treatment significantly inhibited the secretion of IL-1β, IL-6 and TNF. The burden caused by cancer to the global public health has been markedly increasing, as evidenced by an estimate of 9.6 million mortalities in 2018 (around one sixth of the overall worldwide mortalities), around 70% of which being recorded in nations with low to middle income 1,2. The most frequently diagnosed type of cancer in females is breast cancer, being the reason for more than one tenth of annual newly diagnosed cases and the second cause of mortality. In Iraq, breast cancer occupies the second rank, following cardiovascular diseases, as a major cause of mortality (23% of cancer-related deaths in women) 3. Direct administration of the available chemical drugs is generally problematic, while these chemicals often exert toxicity and unwanted side effects to the patient's

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Section: Acridine Orange and Propidium Iodide Staining The Hsp-aunpsmentioning

confidence: 99%

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Sulaiman

Waheeb

Jabir

et al. 2020

Sci Rep

Self Cite

185

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“…This phenomenon of 2D TMDCs is similar to that of graphene, which can also cause different degrees of cell function disturbance in a variety of cells, including ROS, proliferation and apoptosis (Pelin et al, 2018;Alsaedi et al, 2019;. For example, graphene induced autophagy by activating NFκB and TLR signaling pathways in THP-1 cells and RAW 264.7 cells, respectively.…”

Section: Biosafety Evaluation Of 2d Tmdcsmentioning

confidence: 76%

Two-Dimensional Transition Metal Dichalcogenides: Synthesis, Biomedical Applications and Biosafety Evaluation

Zhou

Sun

Bai

2020

Front. Bioeng. Biotechnol.

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Recently, two-dimensional transition metal dichalcogenides (2D TMDCs) have drawn certain attentions in many fields. The unique and diversified electronic structure and ultrathin sheet structure of 2D TMDCs offer opportunities for moving ahead of other 2D nanomaterials such as graphene and expanding the wide application of inorganic 2D nanomaterials in many fields. For a better understanding of 2D TMDCs, one needs to know methods for their synthesis and modification, as well as their potential applications and possible biological toxicity. Herein, we summarized the recent research progress of 2D TMDCs with particular focus on their biomedical applications and potential health risks. Firstly, two kinds of synthesis methods of 2D TMDCs, top-down and bottom-up, and methods for their surface functionalization are reviewed. Secondly, the applications of 2D TMDCs in the field of biomedicine, including drug loading, photothermal therapy, biological imaging and biosensor were summarized. After that, we presented the existing researches on biosafety evaluation of 2D TMDCs. At last, we discussed major research gap in current researches and challenges and coping strategies in future studies.

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“…GraphPad Prism 6 was used for the statistical analysis of the data, which were presented as the mean ± SEM of three replicates per experiment. 26…”

Section: Discussionmentioning

confidence: 99%

<p>Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line</p>

Jabir

Sahib

Taqi

et al. 2020

IJN

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Graphene nanoparticles induces apoptosis in MCF-7 cells through mitochondrial damage and NF-KB pathway

Cited by 48 publications

References 44 publications

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Hesperidin Loaded on Gold Nanoparticles as a Drug Delivery System for a Successful Biocompatible, Anti-Cancer, Anti-Inflammatory and Phagocytosis Inducer Model

Two-Dimensional Transition Metal Dichalcogenides: Synthesis, Biomedical Applications and Biosafety Evaluation

<p>Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line</p>

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