&lt;p&gt;Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line&lt;/p&gt;

Jabir, Majid S.; Sahib, Usama I.; Taqi, Zainab J.; Taha, Ali A.; Sulaiman, Ghassan M.; Albukhaty, Salim; Al-Shammari, Ahmed Majeed; Alwahibi, Mona S.; Soliman, Dina A.; Dewir, Yaser Hassan; Rizwana, Humaira

doi:10.2147/ijn.s276714

Cited by 82 publications

(32 citation statements)

References 59 publications

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“…The nanoscale is important because nanocarrier systems containing anti-cancer drugs can easily target and accumulate within the tumor’s area by exerting cytotoxic effects on proliferating cells [ 33 ]. FTIR confirmed that the prepared NPs contained uncoated SPION, SPION@Au, FA, and CS NPs.…”

Section: Resultsmentioning

confidence: 99%

Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Nanoparticles Coated with Chitosan and Folate for Targeted Antitumor Therapy

Al-Musawi¹,

Albukhaty

Al-Karagoly

et al. 2020

Nanomaterials

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A dual-targeting nanomedicine composed of pH-sensitive superparamagnetic iron oxide core-gold shell SPION@Au, chitosan (CS), and folate (FA) was developed as a doxorubicin (DOX) antitumor medication. Microemulsion was used for preparation and cross-linking conjugation. The characteristics of the designed nanocomposite were studied using atomic force microscopy (AFM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction, UV-visible spectroscopy, Zeta potential and vibrating sample magnetometry (VSM), and Fourier transform infrared spectroscopy. The prepared SPION@Au-CS-DOX-FA nanoparticles (NPs) were spherical with an average diameter of 102.6 ± 7 nm and displayed an elevated drug loading behavior and sustained drug release capacity. The SPION@Au-CS-DOX-FA NPs revealed long term anti-cancer efficacy due to their cytotoxic effect and apoptotic inducing efficiency in SkBr3 cell lines. Additionally, Real-time PCR outcomes significantly showed an increase in BAK and BAX expression and a decrease in BCL-XL and BCL-2. In vivo results revealed that SPION@Au significantly decreased the tumor size in treated mice through magnetization. In conclusion, prepared SPION@Au-CS-DOX-FA could be a beneficial drug formulation for clinical breast cancer treatment.

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Section: Resultsmentioning

confidence: 99%

Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Nanoparticles Coated with Chitosan and Folate for Targeted Antitumor Therapy

Al-Musawi¹,

Albukhaty

Al-Karagoly

et al. 2020

Nanomaterials

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“…The proliferation of the Ag-NPs-treated THP-1, AMJ-13, and HBL cells was investigated. Labeling of the cells was performed using the cell proliferation kit for flow cytometry (CellTrace™ Red-APC) prior to treatment with the AgNPs, in accordance with the kit protocol (Gibco/Thermo Fisher Scientific, Waltham, MA, USA) [34].…”

Section: Cell Proliferation Assaymentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy

et al. 2021

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Annona muricata is one of the most important traditional medicinal plants which contains numerous chemicals that exhibit various pharmacological properties. In this study, silver nanoparticles were prepared using A. muricata peel extract as a reducing agent and the effect was enhanced through A. muricata like pharmaceutical activity. AgNPs formation was confirmed by color changes, UV-visible spectroscopy, SEM, DLS, and XRD. The anti-proliferative activity of AgNPs against THP-1, AMJ-13, and HBL cell lines was studied. Apoptotic markers were tested using AO/EtBr staining assay, cell cycle phases using flowcytometry, and the expression of P53. Autophagy takes an essential part in controlling inflammasome activation by primary bone marrow-derived macrophages (BMDMs). We report novel functions for AgNPs-affected autophagy, represented by the control of the release of IL-1β, caspase-1, adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), and NLRP3 in BMDMs following treatment with LPS+ATP. The current study revealed that the AgNPs inhibited THP-1 and AMJ-13 cell proliferation. Meanwhile, the AgNPs significantly increased autophagy and reduced IL-1b and NLRP3 levels in both in vivo and in vitro models. The secretion of IL-1β was reduced whereas the degradation of NLRP3 inflammasome was enhanced. These findings propose that AgNPs apply an anti-proliferative activity against THP-1 and AMJ-13 cells through the stimulation of apoptosis via mitochondrial damage and induction of p53 protein pathway. In addition, AgNP-induced autophagy reduced the levels of IL-1β and NLRP3 inflammasome activation. This indicated that the AgNPs augment autophagy controlled by the IL-1β pathway via two different novel mechanisms. The first one is regulating activation of the IL-1 β, caspae-1, and ASC, while the second is NLRP3 targeting for lysosomal degradation. Overall, this study suggests that AgNPs could be a potent therapy for various types of cancer and an alternative treatment for preventing inflammation via enhancing autophagy.

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“…Other microfluidic devices for plasma separation have been demonstrated based on diffusion filter/microfilter [ 19 ], bends in micro-channels [ 20 ], acoustic waves [ 21 ], crossflow filtration [ 22 ], and dielectrophoresis [ 23 , 24 ]. Major research projects in recent decades have focused on microfluidics, drug, and gene nano delivery systems, tissue engineering, and biosensors due to cost-effectiveness and high performance [ 25 , 26 , 27 , 28 , 29 ]. Recently, we developed a microfluidic device featuring two nano-junctions with different sizes to form a size and mobility trap (SMT) for on-chip filtrate the whole blood from blood cells and plasma proteins, then on-chip extract, concentrate, and separate small molecules from whole blood [ 30 ].…”

Section: Introductionmentioning

confidence: 99%

A Novel Microfluidic Device for Blood Plasma Filtration

et al. 2021

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The use of whole blood and some biological specimens, such as urine, saliva, and seminal fluid are limited in clinical laboratory analysis due to the interference of proteins with other small molecules in the matrix and blood cells with optical detection methods. Previously, we developed a microfluidic device featuring an electrokinetic size and mobility trap (SMT) for on-chip extract, concentrate, and separate small molecules from a biological sample like whole blood. The device was used to on-chip filtrate the whole blood from the blood cells and plasma proteins and then on-chip extract and separate the aminoglycoside antibiotic drugs within 3 min. Herein, a novel microfluidic device featuring a nano-junction similar to those reported in the previous work formed by dielectric breakdown was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield of 62% within less than 5 min. The filtered plasma was analyzed using our previous device to show the ability of this new device to remove blood cells and plasma proteins. The filtration device shows a high yield of plasma allowing it to detect a low concentration of analytes from the whole blood.

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<p>Linalool-Loaded Glutathione-Modified Gold Nanoparticles Conjugated with CALNN Peptide as Apoptosis Inducer and NF-κB Translocation Inhibitor in SKOV-3 Cell Line</p>

Cited by 82 publications

References 59 publications

Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Nanoparticles Coated with Chitosan and Folate for Targeted Antitumor Therapy

Design and Synthesis of Multi-Functional Superparamagnetic Core-Gold Shell Nanoparticles Coated with Chitosan and Folate for Targeted Antitumor Therapy

Green Synthesis of Silver Nanoparticles Using Annona muricata Extract as an Inducer of Apoptosis in Cancer Cells and Inhibitor for NLRP3 Inflammasome via Enhanced Autophagy

A Novel Microfluidic Device for Blood Plasma Filtration

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