Graphene–gold nanoparticle composite: Application as a good scaffold for construction of glucose oxidase biosensor

Sabury, Sina; Kazemi, Sayed Habib; Sharif, Farhad

doi:10.1016/j.msec.2015.01.018

Cited by 49 publications

(25 citation statements)

References 69 publications

(59 reference statements)

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“…By combination of graphene with metal nanoparticles, a novel class of carbon-based functional nanomaterials has been developed to optimize the electrical, optical, biological, and catalyst properties because graphene possesses large interfacial surface area [32,[36][37][38][39][40][41].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical sensor for Isoniazid based on the glassy carbon electrode modified with reduced graphene oxide–Au nanomaterials

Guo

Wang

et al. 2015

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 99%

Electrochemical sensor for Isoniazid based on the glassy carbon electrode modified with reduced graphene oxide–Au nanomaterials

Guo

Wang

et al. 2015

Materials Science and Engineering: C

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“…in contrast with our current study which thoroughly addressed cytotoxicity and proliferation for bone cells [65][66][67]. Our results have potentials and implications for future work on osteogenic differentiation and assessing the in vitro and in vivo effects of electrical conductivity and exogenous electrical signaling, stemming partly from successful embedment of gold nanoparticles.…”

Section: Discussionmentioning

confidence: 65%

Simple and Robust Fabrication and Characterization of Conductive Carbonized Nanofibers Loaded with Gold Nanoparticles for Bone Tissue Engineering Applications

Nekounam

Gholizadeh

Allahyari

et al. 2020

Preprint

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Bone tissue engineering is a new and applicable emerging approach to repair the bone defects. In this regard, designing and robust fabrication of tissue engineering scaffolds that could provide an appropriate environment for cell proliferation and differentiation is of high interest.Electrical conductive scaffolds which provide a substrate for stimulating cell growth and differentiation through a physiologically relevant physical signaling, electrical stimulation, has shown a highly promise in this approach. In this paper, we fabricated carbon nanofiber/gold nanoparticle (CNF/GNP) conductive scaffolds using two distinct methods; blending electrospinning in which gold nanoparticles were blended with electrospinning solution and electrospun, and electrospinning/electrospraying in which gold nanoparticle was electrosprayed simultaneously with electrospinning. The obtained electrospun mats underwent stabilization/carbonization process to prepare CNF/GNP scaffolds. The scaffolds were characterized by SEM, XRD, and Raman spectroscopy. SEM characterizations showed improved morphology and a slight decrease in the diameter of the spinned and sprayed nanofibers with moderate concentrations (from 178.66 ± 38.40 nm to 157.94 ± 24.14 nm and 120.81 ± 13.77 nm, respectively), In the electrosprayed form, better size distributions of nanofibers and less adhesion between individual fibers was observed, while XRD analysis confirmed the crystal structure of the nanofibers. Raman spectroscopy revealed enhancement in the graphitization of the structure, and the electrical conductivity of the structure improved by up to 29.2% and 81% in electrospraying and blending electrospinning modes, respectively. Indirect MTT and LDH toxicity assays directly were performed to assess MG63 cell toxicity, but no significant toxicity was observed and the scaffolds did not adversely affect cell proliferation.Overall, it can be concluded that in early tests, this structure have significant potential for bone tissue engineering applications.

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“…In this study, MB was immobilized on the SPE to produce reagentless biosensor so that no need for the introduction of the redox mediator for measurement purposes. Graphene that are composed of a monolayer sp 2 -bonded carbon atoms packed into benzene ring configuration are widely used for sensor and biosensor fabrication due to its fast electron transfer and excellent electrical conductivity (Chen et al 2015;Gholivand & Khodadadian 2014;Sabury et al 2015). In our study, the reduced graphene oxide (rGO) has been used to improve the conductivity of the carbon electrode.…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of Cellulose Nanomaterial for Urea Biosensor Application

Khalid¹,

Lee²,

Arip³

2018

JSM

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Cellulose nanomaterial with rod-like structure and highly crystalline order, usually formed by elimination of the amorphous region from cellulose during acid hydrolysis. Cellulose nanomaterial with the property of biocompatibility and nontoxicity can be used for enzyme immobilization. In this work, urease enzyme was used as a model enzyme to study the surface modification of cellulose nanomaterial and its potential for biosensor application. The cellulose nanocrystal (CNC) surface was modified using 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO)-mediated oxidation to introduce the carboxyl group at C6 primary alcohol. The success of enzyme immobilization and surface modification was confirmed using chemical tests and measured using UV-Visible spectrophotometer. The immobilization strategy was then applied for biosensor application for urea detection. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques were used for electroanalytical characterization of the urea biosensor.

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Graphene–gold nanoparticle composite: Application as a good scaffold for construction of glucose oxidase biosensor

Cited by 49 publications

References 69 publications

Electrochemical sensor for Isoniazid based on the glassy carbon electrode modified with reduced graphene oxide–Au nanomaterials

Electrochemical sensor for Isoniazid based on the glassy carbon electrode modified with reduced graphene oxide–Au nanomaterials

Simple and Robust Fabrication and Characterization of Conductive Carbonized Nanofibers Loaded with Gold Nanoparticles for Bone Tissue Engineering Applications

Surface Modification of Cellulose Nanomaterial for Urea Biosensor Application

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