Graphene-Based SELDI Probe with Ultrahigh Extraction and Sensitivity for DNA Oligomer

Tang, Lena Ai Ling; Wang, Junzhong; Loh, Kian Ping

doi:10.1021/ja104017y

Cited by 266 publications

(203 citation statements)

References 11 publications

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“…Previous report suggested that plain GO can strongly bind to single‐strand DNA via π–π stacking whereas the interaction between double‐stranded DNA and GO is relatively weak 21. Thus, we adopted PEI, the “golden standard” cationic polymer in gene transfection for delivery agent.…”

Section: Resultsmentioning

confidence: 99%

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

et al. 2017

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The objective of this study is to design a graphene‐based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex loaded with miR‐7b overexpression plasmid. GO‐PEI complex exhibits excellent transfection efficiency within the acceptable range of cytotoxicity. The overexpression of miR‐7b after GO‐PEI‐miR‐7b transfection significantly abrogates osteoclast (OC) fusion and bone resorption activity by hampering the expression of an essential fusogenic molecule dendritic cell‐specific transmembrane protein. However, osteoclastogenesis occurs without cell–cell fusion and preosteoclast (POC) is preserved. Through preservation of POC, GO‐PEI‐miR‐7b transfection promotes mesenchymal stem cell osteogenesis and endothelial progenitor cells angiogenesis in the coculture system. Platelet‐derived growth factor‐BB secreted by POC is increased by GO‐PEI‐miR‐7b both in vitro and in vivo. In treating osteoporotic ovariectomized mice, GO‐PEI‐miR‐7b significantly enhances bone mineral density, bone volume as well as bone vascularization through increasing CD31hiEmcnhi cell number. This study provides a cell–cell fusion targeted miRNA transfection drug delivery strategy in treating bone disorders with excessive osteoclastic bone resorption.

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Section: Resultsmentioning

confidence: 99%

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

et al. 2017

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“…Derivatives of graphene are considered as useful matrices that can be replaced with organic matrices that are used conventionally for laser desorption-ionization mass spectrometry (LDI-MS), as they are very efficient in absorbing and transferring energy to the analytes [15]. The efficacy of graphene is much higher than that of GO and rGO, because of its electron transfer and high heat dissipation properties [144]. The hydrophobic properties of graphene make the affinity probe of LDI-MS more suitable for analysis of trace amounts of analytes having an aromatic structure which includes proteins [144], ssDNA, small molecules [69] and pollutants [145].…”

Section: Graphene In Mass Spectrometrymentioning

confidence: 99%

“…The efficacy of graphene is much higher than that of GO and rGO, because of its electron transfer and high heat dissipation properties [144]. The hydrophobic properties of graphene make the affinity probe of LDI-MS more suitable for analysis of trace amounts of analytes having an aromatic structure which includes proteins [144], ssDNA, small molecules [69] and pollutants [145]. The unique properties of graphene make it an excellent absorbent in solid-phase extraction.…”

Section: Graphene In Mass Spectrometrymentioning

confidence: 99%

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Graphene and graphene oxide as nanomaterials for medicine and biology application

et al. 2018

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Graphene-and graphene oxide-based nanomaterials have gained broad interests in research because of their unique physiochemical properties. The 2D allotropic structure allows it to be used in various biological fields. The biomedical applications of graphene and its composite include its use in gene and small molecular drug delivery. It is further used for biofunctionalization of protein, in anticancer therapy, as an antimicrobial agent for bone and teeth implantation. The biocompatibility of the newly synthesized nanomaterials allows its substantial use in medicine and biology. The current review summarizes the chemical structure and biological application of graphene in various fields.

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“…Use as MALDI matrix is a very interesting application aspect for G. [16][17][18][19][20][21][22][23][24] An ideal MALDI matrix should have strong energy absorption and transfer capability. In this regard, G is a good candidate due to its fast charge carrier mobility [25] and universal and frequency-independent optical absorption properties.…”

mentioning

confidence: 99%

Mildly Oxidized Graphene: Facile Synthesis, Characterization, and Application as a Matrix in MALDI Mass Spectrometry

Liu

Cheng

Jiang

2013

Chemistry A European J

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Graphene (G) is a new allotropic member of carbon with a unique two-dimensional and one-atom-thick sheet structure. It has attracted tremendous research interest in both academic and industrial areas due to its exceptional physical and chemical properties. [1][2][3] However, a serious problem in synthesis and application of G is aggregation. Due to the strong hydrophobic nature of G, G sheets have a strong tendency to aggregate to G clusters or even restack to graphite particles through van der Waals interactions. The resulting G agglomerates are very hard and insoluble in water or organic solvents, making further processing difficult. Since most of the exceptional properties of G can only be achieved with individual sheets, prevention of aggregation of G sheets is of particular importance for its applications.Aggregation of G can be reduced by using stabilizers. [4][5][6] However, attachment of foreign molecules onto G sheets may cause an adverse impact on the applications. As an alternative, graphene oxide (GO) has an excellent water dispersibility and has been used as a substitute for G in many applications. [3] GO is usually obtained by exfoliation of graphite oxide, [7][8] which can be synthesized from graphite. [9] However, the heavy oxidation during the synthesis can severely damage the large delocalized p-electron system of G and cause many imperfections and defects in the GO sheets. Thus, the unique electronic and optical absorption properties of G are seriously compromised in GO. Li et al. [10] reported the preparation of a processable aqueous dispersion of chemically converted G from GO; however, the obtained G dispersion was only stable under basic conditions and the reductant was difficult to remove from the dispersion. Therefore, facile and scalable methods for the synthesis of low-defect, well-dispersed, and clean G sheets are still urgently desired. Inspired by GO, we hypothesized that controlling the oxidation degree of G may provide a feasible approach for this purpose. However, although significant efforts have been made on developing synthetic strategies of G, little attention has been paid to controlling the oxidation degree of G. [11][12] Herein, we report a very simple method for the synthesis of mildly oxidized graphene by oxidizing chemically converted G with diluted nitric acid (2 m; Scheme 1A). We aimed to find a facile approach to prevent the aggregation of G sheets while maintaining the G structure as intact as possible. To distinguish from GO, the produced material is called acid-oxidized graphene (AOG). The AOG showed good dispersibility in water (up to 1 mg mL À1 ). Meanwhile, unlike GO (Scheme 1B), the main framework of G was not disrupted, and thus most of the exceptional properties of G can be maintained. Therefore, the AOG combines the advantages of G and GO while avoiding their disadvantages. Furthermore, the AOG contains no stabilizer molecules and can be highly pure, which is very favorable for its applications.We found that the AOG aqueous dispersion was rather stable. Eve...

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Graphene-Based SELDI Probe with Ultrahigh Extraction and Sensitivity for DNA Oligomer

Cited by 266 publications

References 11 publications

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Graphene and graphene oxide as nanomaterials for medicine and biology application

Mildly Oxidized Graphene: Facile Synthesis, Characterization, and Application as a Matrix in MALDI Mass Spectrometry

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