Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Dou, Ce; Ding, Ning; Luo, Fei; Hou, Tianyong; Cao, Zhen; Bai, Yun; Liu, Chuan; Xu, Jiake; Dong, Shiwu

doi:10.1002/advs.201700578

Cited by 79 publications

(64 citation statements)

References 36 publications

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“…The relative expression of osteoclast marker genes, including Trap , MMP‐9 , DC‐STAMP , ATP6V0D2 , c‐Fos, and NFATc1 , also demonstrated the inhibitory effects of NCF on osteoclastogenesis. DC‐STAMP participates in the fusion process, and blockage of its expression can interrupt preosteoclast fusion . Therefore, the depression of DC‐STAMP might be responsible for the inhibitory effects of NCF on cell‐cell fusion.…”

Section: Discussionmentioning

confidence: 99%

“…DC-STAMP participates in the fusion process, [23][24][25] and blockage of its expression can interrupt preosteoclast fusion. 26 Therefore, the depression of DC-STAMP might be responsible for the inhibitory effects of NCF on cell-cell fusion. In addition, NCF treatment decreased the osteoclast surface/bone surface ratio and preserved bone destruction in OVX mice.…”

Section: F I G U R E 4 Ncf Potentiates the Angiogenesis-related Activmentioning

confidence: 99%

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Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation

et al. 2020

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Recently, type H vessels were reported to couple angiogenesis and osteogenesis during osteoclastogenesis, and tartrate‐resistant acid phosphatase (Trap)+ preosteoclasts were found to secrete increased PDGF‐BB to promote type H vessel formation. Therefore, utilization of type H vessels may be a strategy to treat diseases involving bone loss. In the present study, we found that nuciferine, a natural bioactive compound, has various effects, including inhibiting osteoclastogenesis and promoting type H vessel formation. Nuciferine inhibited osteoclastogenesis and bone resorption but increased the relative number of Trap+ preosteoclasts. Nuciferine restrained the expression of osteoclast‐specific genes and proteins, promoted PDGF‐BB production and potentiated related angiogenic activities by inhibiting the MAPK and NF‐κB signaling pathways in vitro. We confirmed the bone‐protective effects of nuciferine in ovariectomized mice and found that nuciferine treatment increased the PDGF‐BB concentration and the number of type H vessels in the femur. In conclusion, our results demonstrated that nuciferine can decrease multinucleated osteoclast formation and promote type H vessel formation through preservation of Trap+ preosteoclasts via inhibition of the MAPK and NF‐κB signaling pathways and may be an excellent agent for the treatment of diseases involving bone loss.

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Section: Discussionmentioning

confidence: 99%

Section: F I G U R E 4 Ncf Potentiates the Angiogenesis-related Activmentioning

confidence: 99%

Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation

et al. 2020

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“…Cells were removed by the 5% of hypochlorous acid solution. Resorption area was quantified by the ImageJ software as previously described …”

Section: Methodsmentioning

confidence: 99%

“…Resorption area was quantified by the ImageJ software as previously described. 28,29 2.12 | Survival of osteoclasts After 6 days cultures of osteoclasts, RANKL was removed from the culture medium and further cultured for the indicated periods. The remaining TRAP + MNCs were counted, and the survival rate is expressed as a percentage of the remaining cells at the initial time point (0 hour).…”

Section: Assessment Of Bone Resorptionmentioning

confidence: 99%

Bif‐1/Endophilin B1/SH3GLB1 regulates bone homeostasis

Touyama

Khan

Aoki

et al. 2019

J of Cellular Biochemistry

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Skeletal tissue homeostasis is maintained via the balance of osteoclastic bone resorption and osteoblastic bone formation. Autophagy and apoptosis are essential for the maintenance of homeostasis and normal development in cells and tissues. We found that Bax‐interacting factor 1 (Bif‐1/Endophillin B1/SH3GLB1), involving in autophagy and apoptosis, was upregulated during osteoclastogenesis. Furthermore, mature osteoclasts expressed Bif‐1 in the cytosol, particularly the perinuclear regions and podosome, suggesting that Bif‐1 regulates osteoclastic bone resorption. Bif‐1‐deficient (Bif‐1 −/−) mice showed increased trabecular bone volume and trabecular number. Histological analyses indicated that the osteoclast numbers increased in Bif‐1 −/− mice. Consistent with the in vivo results, osteoclastogenesis induced by receptor activator of nuclear factor‐κB (NF‐κB) ligand (RANKL) was accelerated in Bif‐1 −/− mice without affecting RANKL‐induced activation of RANK downstream signals, such as NF‐κB and mitogen‐activated protein kinases (MAPKs), CD115/RANK expression in osteoclast precursors, osteoclastic bone‐resorbing activity and the survival rate. Unexpectedly, both the bone formation rate and osteoblast surface substantially increased in Bif‐1 −/− mice. Treatment with β‐glycerophosphate (β‐GP) and ascorbic acid (A.A) enhanced osteoblastic differentiation and mineralization in Bif‐1 −/− mice. Finally, bone marrow cells from Bif‐1 −/− mice showed a significantly higher colony‐forming efficacy by the treatment with or without β‐GP and A.A than cells from wild‐type (WT) mice, suggesting that cells from Bif‐1 −/− mice had higher clonogenicity and self‐renewal activity than those from WT mice. In summary, Bif‐1 might regulate bone homeostasis by controlling the differentiation and function of both osteoclasts and osteoblasts (235 words).

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“…bonded to cationic polymers such as polyethylenimine (PEI) [12][13][14][15] . In current study, we developed a graphenebased plasmid transformation system using GO-PEI complexes loaded with antisense vicR plasmid (GO-PEI-ASvicR) by electrostatic interactions.…”

Section: Introductionmentioning

confidence: 99%

Nano-graphene oxide with antisense <i>vicR</i> RNA reduced exopolysaccharide synthesis and biofilm aggregation for <i>Streptococcus mutans </i>

Liu

Zhang

et al. 2020

Dent. Mater. J.

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Streptococcus mutans (S. mutans) has been proved to crucial cariogenic pathogens. Antisense vicR RNA reduced the transcription of virulence genes and lead to a reduction in biofilm formation. In the current study, a graphene-oxide plasmid transformation system was developed using interacted GO-polyethylenimine (PEI) complexes loaded with antisense vicR-expressing plasmid (GO-PEI-ASvicR). The particle size distribution and zeta potential of the GO-PEI-based ASvicR were evaluated. Quantitative real-time PCR assays were used to investigate the expression of S. mutans virulence genes. The exopolysaccharide (EPS) production in biofilm were evaluated by confocal laser scanning microscopy and anthrone method. We showed that GO-PEI could efficiently deliver the ASvicR-expressing plasmid into S. mutans cells and support excellent transcripts of ASvicR. Furthermore, GO-PEI-ASvicR significantly reduced virulent-associated gene expressions, suppressed biofilm aggregation and inhibited EPS accumulation. Our reports demonstrated that preserving nano-graphene oxide with antisense vicR RNA will be a more effective strategy for dental caries management.

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Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Cited by 79 publications

References 36 publications

Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation

Nuciferine prevents bone loss by disrupting multinucleated osteoclast formation and promoting type H vessel formation

Bif‐1/Endophilin B1/SH3GLB1 regulates bone homeostasis

Nano-graphene oxide with antisense <i>vicR</i> RNA reduced exopolysaccharide synthesis and biofilm aggregation for <i>Streptococcus mutans </i>

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