Graphene based electrochemical immunosensor for the ultra-sensitive label free detection of Alzheimer's beta amyloid peptides Aβ(1–42)

Abbasi, Hina Y.; Tehrani, Zari; Devadoss, Anitha; Ali, Muhammad Munem; Moradi-Bachiller, Soraya; Albani, Diego; Guy, Owen J.

doi:10.1039/d0na00801j

Cited by 24 publications

(16 citation statements)

References 55 publications

(37 reference statements)

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“…In another investigation, 153 ultrathin layers of polymerized 1,5-diaminonaphthalene (pDAN) were used to coat screenprinted graphene electrodes (SPGEs) to produce a highly sensitive immunosensor for the detection of Aβ peptides, which have been demonstrated to be a reliable AD biomarker. The SPGEs were coated on a continuous polymer layer having a regulated thickness using the electropolymerization of 1,5diaminonaphthalene (DAN).…”

Section: Graphene Against Alzheimer's Diseasementioning

confidence: 99%

Graphene-based Nanomaterials in Fighting the Most Challenging Viruses and Immunogenic Disorders

Ebrahimi

Asadi

Akhavan

2021

ACS Biomater. Sci. Eng.

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Viral diseases have long been among the biggest challenges for healthcare systems around the world. The recent Coronavirus Disease 2019 (COVID-19) pandemic is an example of how complicated the situation can get if we are not prepared to combat a viral outbreak in time, which brings up the need for quick and affordable biosensing platforms and vast knowledge of potential antiviral effects and drug/gene delivery opportunities. The same challenges have also existed for nonviral immunogenic disorders. Nanomedicine is considered a novel candidate for effectively overcoming these worldwide challenges. Among the versatile nanomaterials commonly used in biomedical applications, graphene has recently earned much attention thanks to its special and inspiring physicochemical properties, such as its large surface area, efficient thermal/electrical properties, carbon-based chemical purity with controllable biocompatibility, easy functionalization, capability of single-molecule detection, anticancer characteristics, 3D template feature in tissue engineering, and, in particular, antibacterial/antiviral activities. In this Review, the most important and challenging viruses of our era, such as human immunodeficiency virus, Ebola, SARS-CoV-2, norovirus, and hepatitis virus, and immunogenic disorders, such as asthma, Alzheimer’s disease, and Parkinson’s disease, in which graphene-based nanomaterials can effectively take part in the prevention, detection, treatment, medication, and health effect issues, have been covered and discussed.

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Section: Graphene Against Alzheimer's Diseasementioning

confidence: 99%

Graphene-based Nanomaterials in Fighting the Most Challenging Viruses and Immunogenic Disorders

Ebrahimi

Asadi

Akhavan

2021

ACS Biomater. Sci. Eng.

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“…Among all form of amyloid peptides, β‐Amyloid Peptide (Aβ 1‐42 ) is more toxic and amyloidogenic and can be effectively used as a marker to identify the disease [2] . Aβ 1‐42 is present in cerebrospinal fluid (CSF) and blood plasma and are lower in CSF of AD patients than healthy control [3] …”

Section: Introductionmentioning

confidence: 99%

“…[2] Aβ 1-42 is present in cerebrospinal fluid (CSF) and blood plasma and are lower in CSF of AD patients than healthy control. [3] Conventional analytical protocols such as enzyme-linked immunosorbent assay and other imaging techniques are expensive, time consuming and need sophisticated lab environment and pose problems concerning lower limit of detection. [4][5][6] Thus, there is an immediate need for the development of ultra-sensitive, low-cost, easy to use and less time consuming detection method for selective, and effective detection of bioanalytes and disease biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Single‐platform, Attomolar Detection of Multiple Biomarkers by a Flexible SERS Sensor

Verma

Jaiswal

Naqvi

et al. 2023

Chemistry An Asian Journal

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Early detection of Alzheimer's disease (AD) is critical for better healthcare management. Herein, we demonstrate a Surface Enhanced Raman Spectroscopy (SERS) active sensor for highly sensitive and selective detection of β-Amyloid Peptide (Aβ 1-42 ), a biomarker of Alzheimer's disease. Polyacrylonitrile (PAN) nanofiber mats, containing purine-based ligand (L; 0 mg (P 1 ), 50 mg (P 2 ), and 100 mg (P 3 )) were prepared by electrospinning followed by functionalization with silver nanoparticles (AgNPs). The fabricated SERS sensors were employed for the detection of Rhodamine 6G (Rh-6G) dye for optimization and the highest sensitivity was achieved on P 3 /AgNPs SERS sensor. The P 3 /AgNPs sensor was chosen for the detection of Aβ 1-42 and human Insulin (HI). The limit of detection (LoD) was found to be 76 × 10 À 18 M and 26 × 10 À 18 M for Aβ 1-42 and HI, respectively. The sensitivity achieved is one order improved for Aβ 1-42 and four orders for HI when compared with reported values. Also, demonstrated the selectivity of the P 3 /AgNPs sensor by testing a simulated cerebrospinal fluid (CSF) and achieved easily identifiable peaks of Aβ 1-42 among the noise of HI and bovine serum albumin should be written before the acronym of BSA. This approach could be extended to develop ultra-sensitive flexible SERS sensors for the facile detection of multiple biomarkers on a single platform with excellent sensitivity, selectivity and stability.

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“…The distinguishing feature of Alzheimer’s disease is the presence of β-amyloid (Aβ) that build up in the brain to the point that they obstruct normal cognitive functions. This usually manifests with changes in memory, abstract thinking, judgement, behavior, and emotions, and it ultimately interferes with physical control over the living body [ 2 , 3 , 4 , 5 , 6 ]. Thus, it is of great significance to achieve accurate monitoring of Aβ in the early stage of AD for prevention and treatment.…”

Section: Introductionmentioning

confidence: 99%

Versatile Dicyanomethylene-Based Fluorescent Probes for the Detection of β-Amyloid in Alzheimer’s Disease: A Theoretical Perspective

Zhang

et al. 2022

IJMS

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Motivated by the growing demand for target chemosensors designed with diagnostic or therapeutic capability for fibrils related to amyloidosis diseases, we investigated in the present work the response mechanism of dicyanomethylene-based fluorescent probes for amyloid fibril using a combined approach, including molecular docking, quantum mechanics/molecular mechanics (QM/MM), and the quantum chemical method. Various binding modes for the probes in β-amyloid (Aβ) are discussed, and the fibril environment-induced molecular optical changes at the most stable site are compared to the fibril-free situation in aqueous environments. The results reveal that the fluorescence enhancement for the probes in Aβ observed experimentally is an average consequence over multiple binding sites. In particular, the conformational difference, including conjugation length and donor effect, significantly contributes to the optical property of the studied probes both in water and fibril. To further estimate the transition nature of the molecular photoabsorption and photoemission processes, the hole-electron distribution and the structural variation on the first excited state of the probes are investigated in detail. On the basis of the calculations, structure–property relationships for the studied chemosensors are established. Our computational approach with the ability to elucidate the available experimental results can be used for designing novel molecular probes with applications to Aβ imaging and the early diagnosis of Alzheimer’s disease.

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Graphene based electrochemical immunosensor for the ultra-sensitive label free detection of Alzheimer's beta amyloid peptides Aβ(1–42)

Abstract: An immunosensor, capable of high sensitivity detection of beta-amyloid peptides, shown to be a reliable biomarker for Alzheimer’s disease, has been developed using screen printed graphene electrodes (SPGEs) modified with...

Cited by 24 publications

References 55 publications

Graphene-based Nanomaterials in Fighting the Most Challenging Viruses and Immunogenic Disorders

Graphene-based Nanomaterials in Fighting the Most Challenging Viruses and Immunogenic Disorders

Single‐platform, Attomolar Detection of Multiple Biomarkers by a Flexible SERS Sensor

Versatile Dicyanomethylene-Based Fluorescent Probes for the Detection of β-Amyloid in Alzheimer’s Disease: A Theoretical Perspective

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