Grapevine fatty acid hydroperoxide lyase generates actin-disrupting volatiles and promotes defence-related cell death

Akaberi, Sahar; Wang, Hao; Claudel, Patricia; Riemann, Michael; Hause, Bettina; Hugueney, Philippe; Nick, Peter

doi:10.1093/jxb/ery133

Cited by 17 publications

(18 citation statements)

References 69 publications

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“…6 ). In grapevine cells, RboH participates in defence-related cell death in response to the bacterial elicitor harpin ( Chang and Nick, 2012 ), or the product of plant oxylipin metabolism, 3- cis -hexenal ( Akaberi et al , 2018 ), and was also found to be necessary for the resulting gene activation ( Chang et al , 2011 ). Thus, O -methylmellein as an amplifier shares a specific signature (dependence on RboH, independence from calcium influx) with cell-death-related defence, while not culminating in cell death.…”

Section: Discussionmentioning

confidence: 99%

“…There is a further, very specific, hallmark of defence-related cell death: massive and rapid remodelling of actin filaments that has been found for the response to both harpin ( Chang et al , 2015 ) and 3- cis -hexenal ( Akaberi et al , 2018 ). We checked this phenomenon and found that O -methylmellein induced actin remodelling, and more importantly, boosted actin remodelling in response to flg22 in a synergistic manner ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Suspension cell cultures of V. rupestris expressing the fluorescent tag green fluorescent protein (GFP) linked with the tubulin marker AtTUB6 ( Guan et al , 2015 ), and V. vinifera L. cv. ‘Chardonnay’ expressing the actin marker Fimbrin Actin-Binding Domain 2 ( FABD2)–GFP were used in this experiment and cultivated as described previously ( Wang and Nick, 2017 ; Akaberi et al , 2018 ). If not stated otherwise, all these treatments were conducted with cells at the onset of the expansion phase (day 4 after subcultivation).…”

Section: Methodsmentioning

confidence: 99%

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Hunting modulators of plant defence: the grapevine trunk disease fungus Eutypa lata secretes an amplifier for plant basal immunity

Guan

Schmidt

et al. 2020

Journal of Experimental Botany

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Grapevine trunk diseases (GTDs) are progressively affecting vineyard longevity and productivity worldwide. To be able to understand and combat these diseases, we need a different concept of the signals exchanged between the grapevine and fungi than the well-studied pathogen-associated molecular pattern and effector concepts. We screened extracts from fungi associated with GTDs for their association with basal defence responses in suspension cells of grapevine. By activity-guided fractionation of the two selected extracts, O-methylmellein was identified as a candidate modulator of grapevine immunity. O-Methylmellein could not induce immune responses by itself (i.e. does not act as an elicitor), but could amplify some of the defence responses triggered by the bacterial elicitor flg22, such as the induction level of defence genes and actin remodelling. These findings show that Eutypa lata, exemplarily selected as an endophytic fungus linked with GTDs, can secrete compounds that act as amplifiers of basal immunity. Thus, in addition to elicitors that can trigger basal immunity, and effectors that down-modulate antibacterial basal immunity, once it had been activated, E. lata seems to secrete a third type of chemical signal that amplifies basal immunity and may play a role in the context of consortia of mutually competing microorganisms.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Hunting modulators of plant defence: the grapevine trunk disease fungus Eutypa lata secretes an amplifier for plant basal immunity

Guan

Schmidt

et al. 2020

Journal of Experimental Botany

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“…Even in non-photosynthetic cells as tobacco BY-2, plastids are present. Although their inner membranes are reduced, the plastids of suspension cells are metabolically active, and trigger, in response to salt stress, the synthesis of the plant stress hormone jasmonic acid , 65 , 66 . Jasmonate signalling clearly responds to peptoid treatment.…”

Section: Outlook: What Is the Role Of Plastids?mentioning

confidence: 99%

A mitochondria-targeted coenzyme Q peptoid induces superoxide dismutase and alleviates salinity stress in plant cells

Asfaw

Liu

et al. 2020

Sci Rep

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Salinity is a serious challenge to global agriculture and threatens human food security. plant cells can respond to salt stress either by activation of adaptive responses, or by programmed cell death. the mechanisms deciding the respective response are far from understood, but seem to depend on the degree, to which mitochondria can maintain oxidative homeostasis. Using plant peptoQ, a Trojan Peptoid, as vehicle, it is possible to transport a coenzyme Q10 (CoQ10) derivative into plant mitochondria. We show that salinity stress in tobacco BY-2 cells (Nicotiana tabacum L. cv Bright Yellow-2) can be mitigated by pretreatment with plant PeptoQ with respect to numerous aspects including proliferation, expansion, redox homeostasis, and programmed cell death. We tested the salinity response for transcripts from nine salt-stress related-genes representing different adaptive responses. While most did not show any significant response, the salt response of the transcription factor NtNAC, probably involved in mitochondrial retrograde signaling, was significantly modulated by the plant peptoQ. Most strikingly, transcripts for the mitochondrial, Mn-dependent Superoxide Dismutase were rapidly and drastically upregulated in presence of the peptoid, and this response was disappearing in presence of salt. the same pattern, albeit at lower amplitude, was seen for the sodium exporter SOS1. The findings are discussed by a model, where plant PeptoQ modulates retrograde signalling to the nucleus leading to a strong expression of mitochondrial SoD, what renders mitochondria more resilient to perturbations of oxidative balance, such that cells escape salt induced cell death and remain viable. By the year 2050, world agriculture should be able to boost production of food crops by 70% to feed the projected 9.1 billion people 1. Salt stress is one of the major challenges to this effort and has already affected 20% of cultivated land worldwide. Global warming and suboptimal irrigation accentuate the problem 2,3. The molecular, cellular, and physiological mechanisms underlying the severe effect of salinity on plant growth, development and yield have been reviewed comprehensively 3-5. Based on their response to salt stress, plants are categorised into the tolerant halophytes and the sensitive glycophytes. Unfortunately, most of the crop species belong to the

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“…The GLV aldehydes derived from HPL branch of oxylipin pathway are involved in a complex signaling system during plant-plant and plant-insect interactions , and provide enhanced protection against external environmental stresses (Bisignano et al, 2001;. In grapevine, cis-3-hexenal elicits a rapid disintegration of actin filaments and promotes defence-related cell death (Akaberi et al, 2018). The volatile aldehydes are also believed to behave as "volatile phytoalexins".…”

Section: Introductionmentioning

confidence: 99%

Hydroperoxide lyase modulates defense response and confers lesion‐mimic leaf phenotype in soybean (Glycine max (L.) Merr.)

Wang

Liu

et al. 2020

The Plant Journal

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Summary Allene oxide synthase (AOS) and hydroperoxide lyase (HPL) are two important members of P450 enzymes metabolizing hydroperoxy fatty acid to produce jasmonates and aldehydes respectively, which function in response to diverse environmental and developmental stimuli. However, their exact roles in soybean have not been clarified. In present study, we identified a lesion‐mimic mutant in soybean named NT302, which exhibits etiolated phenotype together with chlorotic and spontaneous lesions on leaves at R3 podding stage. The underlying gene was identified as GmHPL encoding hydroperoxide lyase by map‐based cloning strategy. Sequence analysis demonstrated that a single nucleotide mutation created a premature termination codon (Gln20‐Ter), which resulted in a truncated GmHPL protein in NT302. GmHPL RNA was significantly reduced in NT302 mutant, while genes in AOS branch of the 13‐LOX pathway were up‐regulated in NT302. The mutant exhibited higher susceptibility to bacterial leaf pustule (BLP) disease, but increased resistance against common cutworm (CCW) pest. GmHPL was significantly induced in response to MeJA, wounding, and CCW in wild type soybean. Virus induced gene silencing (VIGS) of GhHPL genes gave rise to similar lesion‐mimic leaf phenotypes in upland cotton, coupled with upregulation of the expression of JA biosynthesis and JA‐induced genes. Our study provides evidence that competition exist between HPL and AOS branches in 13‐LOX pathway of the oxylipin metabolism in soybean, thereby plays essential roles in modulation of plant development and defense.

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Grapevine fatty acid hydroperoxide lyase generates actin-disrupting volatiles and promotes defence-related cell death

Abstract: A fatty acid hydroperoxide lyase from grapevine promotes defence-related cell death and generates 3-cis-hexenal, which specifically activates actin disruption.

Cited by 17 publications

References 69 publications

Hunting modulators of plant defence: the grapevine trunk disease fungus Eutypa lata secretes an amplifier for plant basal immunity

Hunting modulators of plant defence: the grapevine trunk disease fungus Eutypa lata secretes an amplifier for plant basal immunity

A mitochondria-targeted coenzyme Q peptoid induces superoxide dismutase and alleviates salinity stress in plant cells

Hydroperoxide lyase modulates defense response and confers lesion‐mimic leaf phenotype in soybean (Glycine max (L.) Merr.)

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