Grape seed proanthocyanidins repress the hepatic lipid regulators miR‐33 and miR‐122 in rats

Abstract

These results suggest that proanthocyanidin treatment increased hepatic cholesterol efflux to produce new HDL particles by repressing miR-33, and it reduced lipogenesis by repressing miR-122. These results highlight a new mechanism by which grape seed proanthocyanidins produce hypolipidemia through their effects on miRNA modulators of lipid metabolism.

A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor γ with miR-27 involved mechanism

“…And the dosages of proanthocyanidins used in animal models ranged from 10-250 mg/kg body weight. It was reported that a concentration of 25 μg/ml of proanthocyanidins is need in vitro to reach the significant regulation of miRNA [56]. Results from Qiu et alindicated that after a single oral administration of 100 mg/kg body weight for each compound, the plasma concentrations of EGC and EGCG dimer were 7.5 and 9.3 nmol/L, separately [57].…”

“…Results from Qiu et alindicated that after a single oral administration of 100 mg/kg body weight for each compound, the plasma concentrations of EGC and EGCG dimer were 7.5 and 9.3 nmol/L, separately [57]. and their metabolites (about 17.18 μmol/L in total) were five times higher than that in the cell media at 25 μg/ml of proanthocyanidins [56]. Therefore, the concentrations (20-60 μg/ml) we used in the present study were enough to modulate miRNA levels in vivo.…”

Proanthocyanidin Protects Human Embryo Hepatocytes from Fluoride-Induced Oxidative Stress by Regulating Iron Metabolism

“…The doses of NaF and GSPE were selected based on previous studies conducted in our laboratory [14,15].…”

Persimmon tannin alleviates hepatic steatosis in L02 cells by targeting miR-122 and miR-33b and its effects closely associated with the A type ECG dimer and EGCG dimer structural units

“…Recently, it was reported that miR-122 and miR-33a/b played a major role in regulating fatty acids and cholesterol metabolism (Baselga-Escudero et al, 2012;Esau et al, 2006;Rayner et al, 2011). miR-122 is a highly liver-specific miRNA, making up 70% of the total miRNA population in liver (Wen & Friedman, 2012).…”