Granulocyte colony-stimulating factor attenuates early ventricular expansion after experimental myocardial infarction

Sugano, Yasuo; Anzai, Toshihisa; Yoshikawa, Tsutomu; Maekawa, Yuichiro; Kohno, Takashi; Mahara, Keitaro; Naito, Kunihiko; Ogawa, Satoshi

doi:10.1016/j.cardiores.2004.10.008

Cited by 89 publications

(93 citation statements)

References 50 publications

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“…We previously reported that G-CSF treatment improved (22) and GM-CSF induction aggravated (23) early LV remodeling after MI through modification of the infarct healing process. GM-CSF induces differentiation from immature DC to myeloid DC, with a subsequent increase in Th1 cells, whereas G-CSF induces plasmacytoid DC followed by proliferation of Th2 cells (24 -26).…”

Section: Eft Ventricular (Lv)mentioning

confidence: 96%

Differential Effects of GM-CSF and G-CSF on Infiltration of Dendritic Cells during Early Left Ventricular Remodeling after Myocardial Infarction

Naito

Anzai

Sugano

et al. 2008

The Journal of Immunology

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Several lines of evidence suggest that the immune activation after myocardial infarction (MI) induces secondary myocardial injury. Although dendritic cells (DC) are potent regulators of immunity, their role in MI is still undetermined. We investigated the effect of DC modulation by CSF on left ventricular (LV) remodeling after MI. MI was induced by ligation of the left coronary artery in male Wistar rats. G-CSF (20 μg/kg/day, MI-G, n = 33), a GM-CSF inducer (romurtide, 200 μg/kg/day, MI-GM, n = 28), or saline (MI-C, n = 55) was administered for 7 days. On day 14, MI-G animals had higher LV max dP/dt and smaller LV dimensions, whereas MI-GM animals had lower LV max dP/dt and larger LV dimensions than did MI-C animals, despite similar infarct size. In MI-C, OX62+ DC infiltrated the infarcted and border areas, peaking on day 7. Bromodeoxyuridine-positive DC were observed in the border area during convalescence. Infiltration by DC was decreased in MI-G animals and increased in MI-GM animals compared with MI-C (p < 0.05). In the infarcted area, the heat shock protein 70, TLR2 and TLR4, and IFN-γ expression were reduced in MI-G, but increased in MI-GM in comparison with those in MI-C animals. IL-10 expression was higher in MI-G and lower in MI-GM than in MI-C animals. In conclusion, G-CSF improves and GM-CSF exacerbates early postinfarction LV remodeling in association with modulation of DC infiltration. Suppression of DC-mediated immunity could be a new strategy for the treatment of LV remodeling after MI.

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Section: Eft Ventricular (Lv)mentioning

confidence: 96%

Differential Effects of GM-CSF and G-CSF on Infiltration of Dendritic Cells during Early Left Ventricular Remodeling after Myocardial Infarction

Naito

Anzai

Sugano

et al. 2008

The Journal of Immunology

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“…Orlic and colleagues reported that a significant accelerated mobilization of BMSCs occurs after SCF + G-CSF administration resulting in enhanced myocardial regeneration in infarcted murine hearts [8]. The latter investigations have instead proposed myocardial protection of G-CSF through angiogenesis [9] and prevention of scar formation in the left ventricles (LV) [10,11]. It has been reported that BMSCs secrete a wide array of cytokines which may protect target cells [12,13].…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo effects of bone marrow stem cells on cardiac structure and function

Xu¹,

Uemura²,

Dai

et al. 2007

Journal of Molecular and Cellular Cardiology

177

123

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It is hypothesized that the protection of bone marrow stem cells (BMSCs) on ischemic myocardium might be related to the anti-apoptotic effect via paracrine mechanisms. In this study, a wide array of cytokines including vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), stromal cell derived factor-1 (SDF-1) and insulin growth factor-1 (IGF-1) were detected in the BMSCs cultured medium by ELISA. Myocyte apoptosis was assayed by DNA fragmentation and annexin-V staining. Myocardial infarction model was produced by ligation of mouse left anterior descending coronary arteries (LAD). Before LAD ligation, mice were myoablated by irradiation and transplanted with bone marrow cells from transgenic green fluorescent protein (GFP) mice. After LAD ligation, animals were administered stem cell factor (SCF, 200 μg / day / kg, i.p.) or saline for 6 days. Animals were sacrificed on end of SCF treatment and four weeks later. Apoptotic cardiomyocytes were assayed after treatment finished by TUNEL. Myocardial function was analyzed by echocardiography and pressure-volume loop. Bcl-2 protein was analyzed by western blotting. Our results showed that cultured BMSCs released VEGF, bFGF, SDF-1 and IGF-1. Hypoxia induced cell apoptosis was diminished in cardiomyocytes co-cultured with BMSCs. Smaller LV dimension and increased LV ejection fraction were seen in SCF treated animals. SCF significantly reduced cardiomyocytes apoptosis within peri-infarct area and up-regulation expression of Bcl-2 in ischemic area. Moreover, conditioned medium from cultured BMSCs also induced up-regulation of Bcl-2 protein in cardiomyocytes. It is concluded that paracrine mediators secreted by BMSCs might be involved in early repair of ischemic heart by preventing cardiomyocytes apoptosis and improving cardiac function.

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“…Известно, что структура наполнения желудочков сердца зависит от способности миокарда рассла-бляться и растягиваться, от камерной жесткости, механических факторов -величины градиента дав-ления между предсердиями и желудочками, силой сокращения предсердий, от величины давления в предсердиях, величины КДД в желудочках [2,5,9,11,12]. При ИМ нарушение структуры наполнения идет за счет замедления расслабления в сочетании со снижением растяжимости как следствие зон ише-мии миокарда, приводящей как к замедленному уда-лению кальция из миофибрилл, так и снижению содержания АТФ.…”

Section: результаты и обсуждениеunclassified

Place of Volumetric Load in Assessment of Diastolic Reserve of the Left Ventricle and in Prediction of Heart Failure Development for Myocardial Infarction Patients

Бейшенкулов¹,

Abdurashidova²,

Kaliev³

et al. 2016

Russ J Cardiol

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ОРИГИНАЛЬНЫЕ СТАТЬИ 31У больных инфарктом миокарда (ИМ), в условиях острой ишемии миокарда, нарушение диастоличе-ской функции (ДФ) левого желудочка (ЛЖ) обычно сопровождает снижение его насосной функции, а иногда предшествует ему [1][2][3]. Известно о роли диастолической дисфункции (ДД) в возникновении, клиническом течении и прогнозе застойной сердеч-ной недостаточности (СН) [4][5][6]. Изменение параме-РОЛь ОБЪЕМНОЙ НАГРУЗКИ В ОЦЕНКЕ ДИАСТОЛИЧЕСКОГО РЕЗЕРВА ЛЕВОГО ЖЕЛУДОЧКА И В ПРОГНОЗИРОВАНИИ РАЗВИТИя СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТИ У БОЛьНЫХ ИНФАРКТОМ МИОКАРДА бейшенкулов М. Т., Абдурашидова Т. Ш., Калиев К. Р., Токтосунова А. К., Тагаева А. К.Цель. изучить роль объемной нагрузки в оценке диастолического резерва левого желудочка (ЛЖ) и выявить информативные предикторы трансмитраль-ного диастолического потока в развитии сердечной недостаточности у боль-ных инфарктом миокарда без систолической дисфункции ЛЖ. Материал и методы. Обследовано 40 больных мужчин первичным Q волно-вым инфарктом миокарда без клинических признаков сердечной недостаточ-ности с исходной фракцией выброса ЛЖ в пределах 40-55%. Статистический анализ проводили с помощью электронных таблиц Excel 5.0. Применяли стан-дартные методы вариационной статистики: вычисление средних величин, стандартных ошибок средней. Достоверность различий определяли с помощью критерия t Стьюдента. Результаты. У больных инфарктом миокарда на объемной нагрузке отмеча-лось 2 типа изменений трансмитрального диастолического потока. 1 группа -больные, у которых на объемной нагрузке изменения трансмитрального диа-столического потока были однонаправлены со здоровыми: достоверно увели-чился Е и А (p<0,05), не изменился Е/А, укоротился IVRt ЛЖ и tdec (p<0,002). 2 группа -больные, у которых на объемной нагрузке отмечалось отсутствие увеличения Е (p>0,05), достоверно увеличился А на 12% (p<0,002) и, следова-тельно, уменьшился Е/А (p<0,05), и достоверно удлинился IVRt ЛЖ и tdec (p<0,05); изменения не возвратились к исходным к 5 мин восстановительного периода. Заключение. 1) нагрузочный тест ОДНТ является безопасным и информатив-ным методом для определения ДР ЛЖ и выявления больных с высоким риском развития застойной СН у больных иМ. 2) у больных со сниженным ДР ЛЖ при ОН отмечается уменьшение Е, достоверное увеличение А, уменьшение Е/А и удлинение IVRt и tdec, в постинфарктный период в 27,3% случаев развива-ется застойная СН. 3) у больных иМ с сохраненным ДР ЛЖ в постинфарктный период застойная СН не развивается. PLACE OF VOLUMEtRIC LOAD IN ASSESSMENt OF DIAStOLIC RESERVE OF tHE LEFt VENtRICLE AND IN PREDICtION OF HEARt FAILURE DEVELOPMENt FOR MYOCARDIAL INFARCtION PAtIENtSBeyshenkulov М. Т., Abdurashidova Т. Sh., Kaliev К. R., toktosunova А. К., tagaeva А. К.Aim. to study the role of volumetric load in assessment of diastolic reserve of the left ventricle (LV) and to reveal informative predictors of transmitral diastolic flow for heart failure development in myocardial infarction patients without systolic LV dysfunction. Material and methods. totally, 40 males studied with primary Q-w...

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Granulocyte colony-stimulating factor attenuates early ventricular expansion after experimental myocardial infarction

Abstract: G-CSF treatment improves early post-infarct ventricular expansion through promotion of reparative collagen synthesis in the infarcted area, suggesting some beneficial effect of G-CSF on the infarct healing process.

Cited by 89 publications

References 50 publications

Differential Effects of GM-CSF and G-CSF on Infiltration of Dendritic Cells during Early Left Ventricular Remodeling after Myocardial Infarction

Differential Effects of GM-CSF and G-CSF on Infiltration of Dendritic Cells during Early Left Ventricular Remodeling after Myocardial Infarction

In vitro and in vivo effects of bone marrow stem cells on cardiac structure and function

Place of Volumetric Load in Assessment of Diastolic Reserve of the Left Ventricle and in Prediction of Heart Failure Development for Myocardial Infarction Patients

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