Gramicidin S messenger RNA

The Biosynthesis of Gramicidin S. A Restudy*

“…Different strains of this organism produce a variety of polypeptides which range from 10 to 17 or 20 amino acids in length. Originally, it appeared that a ribosomal system had been obtained for the synthesis of these peptides (Bodley et al, 1964;Hall et al, 1965), a finding which contradicted reports from other laboratories of a nonribosomal type of pathway (Mach et al, 1963;Eikhom et al, 1964;Yukioka et al, 1965). During the past year, however, it has not been possible to repeat these experiments and the possibility must be considered that the earlier reports should be reassessed.…”

Chromosomal ribonucleic acid of rat ascites cells

“…WAVELENGTH: µ. The fact that chromosomal RNA elutes from DEAE-Sephadex as a single sharp peak indicates that it is composed of molecules of a relatively homogeneous chain length (Hall et al, 1965). The pattern obtained from sucrose density gradient centrifugation of labeled chromosomal RNA in the presence of total ascites figure 3: Sedimentation pattern of 32P-labeled ascites chromosomal RNA in the presence of total ascites RNA.…”

Biosynthesis of gramicidin S. New peptide intermediate

“…The incorporation studies indicated that the X peptide contained all of the amino acids found in gramicidin S, but its mobility on electrophoresis was not consistent with the hypothesis that is was a simple pentapeptide. The pentapeptide produced from gramicidin S by nagarse digestion had been shown to migrate more rapidly than gramicidin S on electrophoresis in 50% acetic acid (Hall et al, 1965). The X peptide, on the contrary, migrated well behind gramicidin S, indicating that one of its amino groups was masked or that is was larger than a pentapeptide.…”