Gram-positive commensal bacteria for mucosal vaccine delivery

Fischetti, Vincent A.; Medaglini, Donata; Pozzi, Gianni

doi:10.1016/s0958-1669(96)80079-6

Cited by 55 publications

(23 citation statements)

References 88 publications

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“…Moreover, the pneumococcal pullulanase is immunogenic and highly conserved, making it a potential target for vaccine development (9). Cell wall sorting signals have also been used to create fusion proteins with heterologous antigens, which can thus be expressed in a gram-positive commensal for oral vaccination (17,40). Moreover, CWPs from gram-positive bacteria are known to interact with human proteins and as such may be used as biotechnological tools.…”

Section: Discussionmentioning

confidence: 99%

Improved Pattern for Genome-Based Screening Identifies Novel Cell Wall-Attached Proteins in Gram-Positive Bacteria

Janulczyk

Rasmussen

2001

Infect Immun

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With a large number of sequenced microbial genomes available, tools for identifying groups or classes of proteins have become increasingly important. Here we present an improved pattern for the identification of cell wall-attached proteins (CWPs), a group of proteins with diverse and important functions in gram-positive bacteria. This tripartite pattern is based on analysis of 65 previously described cell wall-attached proteins and takes into account the three principal requirements for cell wall sorting; a sortase target region (LPXTGX), a membrane-spanning region, and a charged stop-transfer tail. In five different genomes of gram-positive bacteria, the tripartite pattern identified a total of 35 putative CWPs, 19 of which were novel. The specificity and sensitivity of the tripartite pattern are higher than those of the classical pattern, which is based solely on the sortase target region. Several putative CWPs with atypical sortase target regions were identified. In the complete genome of the important human pathogen Streptococcus pyogenes, the tripartite pattern identified 14 putative CWPs. Seven of the putative S. pyogenes proteins were novel, and two of these were a 5 nucleotidase and a pullulanase. This study represents the first whole-genome screening for CWPs, and we conclude that the tripartite pattern is highly suitable for this purpose. Identification of CWPs using this pattern offers important possibilities in the study of the pathogenesis and physiology of gram-positive bacteria.

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Section: Discussionmentioning

confidence: 99%

Improved Pattern for Genome-Based Screening Identifies Novel Cell Wall-Attached Proteins in Gram-Positive Bacteria

Janulczyk

Rasmussen

2001

Infect Immun

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“…Nine healthy, full-term, breast-fed infants-referred to here as infants 1,7,8,12,14,18,20,21, and 24-were selected for this study. They comprised five males and four females.…”

Section: Methodsmentioning

confidence: 99%

“…The saliva samples were held at Ϫ80°C until assay. Saliva collected from infant 18 at 2, 4, and 10 months (visits 4, 5, and 8), from infant 20 at 4, 10, and 15 months (visits 5, 8, and 10), from infant 21 at 2, 4, 8, 10, and 15 months (visits 4, 5, 7, 8, and 10), and from infants 1,7,8,12,14, and 24 at 4 and 15 months (visits 5 and 10) were used in the current study. The volumes of saliva that could be collected, particularly at the earlier visits, were low, such that only a limited number of assays could be carried out on each sample.…”

Section: Methodsmentioning

confidence: 99%

Study of Humoral Immunity to Commensal Oral Bacteria in Human Infants Demonstrates the Presence of Secretory Immunoglobulin A Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 Ribotypes

Cole

Evans

Kirchherr

et al. 2004

Clin Vaccine Immunol

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The mouths of three human infants were examined from birth to age 2 years to detect colonization of Actinomyces naeslundii genospecies 1 and 2. These bacteria did not colonize until after tooth eruption. The diversity of posteruption isolates was determined by ribotyping. Using immunoblotting and enzyme-linked immunosorbent assay, we determined the reactivity of secretory immunoglobulin A (SIgA) antibodies in saliva samples collected from each infant before and after colonization against cell wall proteins from their own A. naeslundii strains and carbohydrates from standard A. naeslundii genospecies 1 and 2 strains. A. naeslundii genospecies 1 and 2 carbohydrate-reactive SIgA antibodies were not detected in any saliva sample. However, SIgA antibodies reactive with cell wall proteins were present in saliva before these bacteria colonized the mouth. These antibodies could be almost completely removed by absorption with A. odontolyticus, a species known to colonize the human mouth shortly after birth. However, after colonization by A. naeslundii genospecies 1 and 2, specific antibodies were induced that could not be removed by absorption with A. odontolyticus. Cluster analysis of the patterns of reactivity of postcolonization salivary antibodies from each infant with antigens from their own strains showed that not only could these antibodies discriminate among strains but antibodies in saliva samples collected at different times showed different reactivity patterns. Overall, these data suggest that, although much of the salivary SIgA antibodies reactive with A. naeslundii genospecies 1 and 2 are directed against genus-specific or more broadly cross-reactive antigens, species, genospecies, and possibly strain-specific antibodies are induced in response to colonization.

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“…On the contrary, it has been suggested that S. gordonii can modulate the virulence properties of S. mutans by interfering with the comCDE quorum-sensing system, which regulates biofilm formation, bacteriocin production and genetic competence (Wang & Kuramitsu, 2005). Because of its pioneer colonizing properties and because of its potential to elicit immune responses in infants and children, it has been proposed to use engineered strains of S. gordonii for live vaccine delivery after mucosal colonization (Fischetti et al, 1996;Lee, 2003). Ideally, such antigens would be exported or displayed on the cell surface of S. gordonii (Lee, 2003).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Streptococcus gordonii prophage PH15: complete genome sequence and functional analysis of phage-encoded integrase and endolysin

Ploeg

2008

Microbiology

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Gram-positive commensal bacteria for mucosal vaccine delivery

Cited by 55 publications

References 88 publications

Improved Pattern for Genome-Based Screening Identifies Novel Cell Wall-Attached Proteins in Gram-Positive Bacteria

Improved Pattern for Genome-Based Screening Identifies Novel Cell Wall-Attached Proteins in Gram-Positive Bacteria

Study of Humoral Immunity to Commensal Oral Bacteria in Human Infants Demonstrates the Presence of Secretory Immunoglobulin A Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 Ribotypes

Characterization of Streptococcus gordonii prophage PH15: complete genome sequence and functional analysis of phage-encoded integrase and endolysin

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