Characterization of Streptococcus gordonii prophage PH15: complete genome sequence and functional analysis of phage-encoded integrase and endolysin

Ploeg, Jan R. van der

doi:10.1099/mic.0.2008/018739-0

Cited by 17 publications

(20 citation statements)

References 42 publications

(40 reference statements)

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“…While some of oral Streptococci such as mutans Streptococci, Streptococcus mutans and Streptococcus sobrinus, are responsible for dental diseases (Milnes et al, 1993;Smith et al, 1993;Jacques, 1998;Van der Ploeg, 2008) others like Streptococcus salivarius, Streptococcus sanguis, Streptococcus oralis and Streptococcus gordonii are the first bacteria that colonize the teeth (Nyvad and Kilian, 1990;Tanzer et al, 2001; Van der Ploeg, 2008). There are nonspecific strategies like tooth brushing and rinsing with biocides for reduction of Streptococcus mutans from dental plaque.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological Applications of Two Novel Lytic Bacteriophages of Streptococcus mutans in Tooth Decay Bio-Controlling

Maal¹,

Bouzari²,

Zavareh³

2015

Current Research in Bacteriology

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Recently the prevention of bacterial infections using their specific viruses, bacteriophages, is challenging. In this research two water samples of Caspian sea were gathered and filtered through 0.45 μm membrane filters. The filtrates were added to Streptococcus mutans culture media in their logarithmic phase, cultivated on brain heart infusion agar and incubated at 37°C and 5% CO 2 until bacteriophage plaques were appeared. Transmission electron microscopy of purified plaques revealed two types of lytic bacteriophages. The first phage had hexagonal head measured 74 nm and was most probably related to Cystoviridae family of bacteriophages. The second lytic bacteriophage contained a bottle-shaped mantle measuring 55×125 nm that was most probably related to Ampullaviridae family of bacteriophages. The microtiter plate assay showed the addition of bacteriophages to Streptococcus mutans after 0, 6, 12 and 18 h of culturing the host strain in microtiter plates prevented the biofilm formation up to 99, 69, 41 and 26%, respectively. In conclusion this is the first report of two novel Cystovirus and Ampullavirus that had lytic effects on Streptococcus mutans. These lytic bacteriophages could be considered as specific agents for phage therapy of tooth decay.

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Section: Introductionmentioning

confidence: 99%

Biotechnological Applications of Two Novel Lytic Bacteriophages of Streptococcus mutans in Tooth Decay Bio-Controlling

Maal¹,

Bouzari²,

Zavareh³

2015

Current Research in Bacteriology

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“…Plasmid pOMZ286 was introduced into E. coli BL21(DE3) (Merck) by transformation. Expression and purification by Ni-NTA agarose affinity chromatography was as described previously [13]. For mass spectrometry analysis, a sample of the protein was purified on C4 ZipTips (Millipore) and measured in 50% acetonitrile/0.2% formic acid (pH 2) on a Q-TOF Ultima API mass spectrometer.…”

Section: Identification Of the Major Capsid Proteinmentioning

confidence: 99%

“…The pellet was dissolved in 0.1 M NH 4 Ac pH 6.7. Electron microscopy was carried out with a Philips EM 400T electron microscope at 60 kV and using phosphotungstic acid as negative stain as described before [13].…”

Section: Preparation Of Phage Lysates and Electron Microscopymentioning

confidence: 99%

“…Interestingly, some virulent S. pneumoniae bacteriophages were capable of replicating in S. oralis [18], highlighting the close relationship between these species. I have previously reported about the presence of prophages in three oral streptococcal strains isolated from human supragingival plaque and about the characterization of bacteriophage PH15, which resides as prophage in the genome of S. gordonii OMZ 1039 [13]. This study deals with prophage PH10 from S. oralis strain OMZ 1038.…”

Section: Introductionmentioning

confidence: 95%

“…Attempts to isolate lytic bacteriophages from human saliva or dental plaque have been successful for some host species, for example for Actinomyces naeslundii [7] and for Streptococcus mutans [8], but others have reported difficulties in isolating bacteriophages [9,10]. The presence of functional prophages in oral microorganisms has been experimentally demonstrated for Aggregatibacter actinomycetemcomitans [11], S. mitis [12], S. gordonii [13] and Treponoma denticola [14].…”

Section: Introductionmentioning

confidence: 99%

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Genome sequence of the temperate bacteriophage PH10 from Streptococcus oralis

Ploeg

2010

Virus Genes

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Exponential growing cultures of Streptococcus oralis strain OMZ 1038, isolated from human supragingival dental plaque, were found to release a bacteriophage (designated PH10) upon treatment with mitomycin C. The complete genome sequence of phage PH10 was determined. The genome was 31276 bp in size and contained 54 open reading frames. The module encoding structural proteins was highly similar to that of Streptococcus pneumoniae prophage PhiSpn_3. The most abundant phage structural protein was encoded by ORF35 and was likely processed by proteolytic cleavage. The putative endolysin from PH10, which contained a muramidase domain and a choline-binding domain, was purified and shown to have lytic activity with S. oralis, S. pneumoniae and Streptococcus mitis, but not with other streptococcal species.

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Implant-Associated Infection

Zimmerli¹,

Trampuž²

2010

Biofilm Infections

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Characterization of Streptococcus gordonii prophage PH15: complete genome sequence and functional analysis of phage-encoded integrase and endolysin

Cited by 17 publications

References 42 publications

Biotechnological Applications of Two Novel Lytic Bacteriophages of Streptococcus mutans in Tooth Decay Bio-Controlling

Biotechnological Applications of Two Novel Lytic Bacteriophages of Streptococcus mutans in Tooth Decay Bio-Controlling

Genome sequence of the temperate bacteriophage PH10 from Streptococcus oralis

Implant-Associated Infection

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