Gram-Negative, Aerobic, Nitrilotriacetate-Utilizing Bacteria from Wastewater and Soil

Egli, Thomas; Weilenmann, Hans Ulrich; El-Banna, Tarek; Auling, Georg

doi:10.1016/s0723-2020(88)80016-x

Cited by 84 publications

(53 citation statements)

References 18 publications

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“…Bootstrap analysis gave a high degree of confidence to this relationship (100 %). Strain FaiI4 T was slightly less closely related to Chelatococcus asaccharovorans (Auling et al, 1993;Egli et al, 1988).…”

Section: Blast Analysis Indicated That Strain Faii4mentioning

confidence: 93%

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Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer

Kanso

Patel

2003

International Journal of Systematic and Evolutionary Microbiology

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Section: Blast Analysis Indicated That Strain Faii4mentioning

confidence: 93%

“…S. K. is a recipient of a Thai Government PhD scholarship. Auling et al (1993) and Egli et al (1988) (C. asaccharovorans) and Das et al (1996) …”

Section: Acknowledgementsmentioning

confidence: 99%

Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer

Kanso

Patel

2003

International Journal of Systematic and Evolutionary Microbiology

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“…The cells were suspended in AUX medium according to the manufacturer's instructions to inoculate the API 50CH system. Utilization of nitrilotriacetate (NTA) was tested with a synthetic medium as described by Egli et al (1988); C. asaccharovorans DSM 6462 T was used as a positive control.…”

mentioning

confidence: 99%

Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Yoon

Kang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106 T , was isolated from wastewater collected from a textile dye works in Korea. Strain K106 T grew optimally at pH 7.0-7.5 and 30-37 6C in the presence of 0-1.0 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain K106 T joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9 %. The predominant ubiquinone of strain K106 T was Q-10. The fatty acid profile of strain K106 T was similar to that of C. asaccharovorans DSM 6462 T . Major polar lipids of strain K106 T and C. asaccharovorans DSM 6462 T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G+C content was 68.3 mol%. Strain K106 T exhibited 16S rRNA gene sequence similarity of 96.6 % to the type strain of C. asaccharovorans. DNA-DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106 T can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106 T represents a novel species of the genus Chelatococcus, for which the name Chelatococcus daeguensis sp. nov. is proposed.

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“…NTA degradation was shown to be a result of microbial processes rather than of photochemical degradation (1,3,6,17,29,33). Several microorganisms, either obligately aerobic or facultatively denitrifying, that can use NTA as a sole source of carbon, nitrogen, and energy have been isolated (3,10,19,23,30,46,53). The majority of the obligately aerobic isolates has been assigned to two new genera, Chelatobacter and Chelatococcus (2).…”

mentioning

confidence: 99%

Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600

1996

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A 6.2-kb DNA fragment containing the genes for the nitrilotriacetate (NTA) monooxygenase of Chelatobacter heintzii ATCC 29600 was cloned and characterized by DNA sequencing and expression studies. The nucleotide sequence contained three major open reading frames (ORFs). Two of the ORFs, which were oriented divergently with an intergenic region of 307 bp, could be assigned to the NTA monooxygenase components A and B. The predicted N-terminal amino acid sequences of these ORFs were identical with those determined for the purified components. We therefore named these genes ntaA (for component A of NTA monooxygenase) and ntaB (for component B). The ntaA and ntaB genes could be expressed in Escherichia coli DH5␣, and the gene products were visualized after Western blotting (immunoblotting) and incubation with polyclonal antibodies against component A or B. By mixing overproduced NtaB from E. coli and purified component A from C. heintzii ATCC 29600, reconstitution of a functional NTA monooxygenase complex was possible. The deduced gene product of ntaA showed only significant homology to SoxA (involved in dibenzothiophene degradation) and to SnaA (involved in pristamycin synthesis); that of ntaB shared weak homologies in one domain with other NADH:flavine mononucleotide oxidoreductases. These homologies provide no conclusive answer as to the possible evolutionary origin of the NTA monooxygenase. The deduced gene product of the third ORF (ORF1) had homology in the N-terminal region with the GntR class of bacterial regulator proteins and therefore may encode a regulator protein, possibly involved in regulation of ntaA and ntaB expression.

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Gram-Negative, Aerobic, Nitrilotriacetate-Utilizing Bacteria from Wastewater and Soil

Cited by 84 publications

References 18 publications

Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer

Microvirga subterranea gen. nov., sp. nov., a moderate thermophile from a deep subsurface Australian thermal aquifer

Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600

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