Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Abstract

Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106 T , was isolated from wastewater collected from a textile dye works in Korea. Strain K106 T grew optimally at pH 7.0-7.5 and 30-37 6C in the presence of 0-1.0 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain K106 T joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9 %. The predominant ubiquinone of strain K106 T was Q-10. The fatty acid profile of strain K106 T was similar to that of C. asaccharovorans DSM 6462 T . Major polar lipids of strain K106 T and C. asaccharovorans DSM 6462 T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G+C content was 68.3 mol%. Strain K106 T exhibited 16S rRNA gene sequence similarity of 96.6 % to the type strain of C. asaccharovorans. DNA-DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106 T can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106 T represents a novel species of the genus Chelatococcus, for which the name Chelatococcus daeguensis sp. nov. is proposed.

The Family Beijerinckiaceae

“…All members of the family Beijerinckiaceae are aerobic but C. daeguensis and C. sambhunathii can also grow anaerobically with nitrate (Panday and Das 2010;Yoon et al 2008). Growth factor requirements have been only reported in two species, C. asaccharovorans ) and M. ligni (Vorob'ev et al 2009).…”

“…However, the next two species of the genus, each based on a single strain, were obtained following a more general approach. C. daeguensis K106 T (Yoon et al 2008) was isolated from wastewater of a textile dye works in Korea, by means of the standard dilution-plating technique on trypticase soy agar (TSA; Difco) at 30 C. C. sambhunathii HT4 T (Panday and Das 2010) was retrieved from sediment of an Indian hot spring that was inoculated into a 250 ml conical flask containing 50 ml nutrient broth (Difco) and incubated on a shaker at 200 r.p.m. and 45 C. After 2 days incubation, the contents of the flasks were serially diluted and plated onto nutrient agar medium (Difco) and incubated at 37 C for 4 days, after which colonies were picked and purified by repeated streaking on the same medium.…”

“…Several characteristics of the genus make . Table 4.4 Differential traits between C. asaccharovorans , C. daeguensis (Yoon et al 2008) it unique compared with other methanotrophic bacteria. Representatives of this genus do not contain an intracytoplasmic membrane system like that in all other known methanotrophs and appear to possess only a soluble form of methane monooxygenase (sMMO).…”

“…Description of the genus Chelatococcus is based on Auling et al (1993) and the emendation by Yoon et al (2008). Cells are cocci .…”

Emended description of the family Beijerinckiaceae and transfer of the genera Chelatococcus and Camelimonas to the family Chelatococcaceae fam. nov.

“…At present, it includes four species, i.e. Chelatococcus asaccharovorans (Auling et al, 1993), Celatococcus daeguensis (Yoon et al, 2008), Chelatococcus sambhunathii (Panday & Das, 2010) and Chetaltococcus caeni (Jin et al, 2015). Members of this genus are neutrophilic short rods, which differ with regard to utilization of different multicarbon substrates.…”