Five winter wheat cultivars created in Small Grains Research Centre of Kragujevac (Ana Morava, Toplica, Vizija, Takovčanka and Lazarica) were grown at the macro field trial in three locations (Kragujevac, Sombor and Bačka Topola) during three years (2004-2006). Influence of genetic and agro-ecological conditions of locations on wheat quality components (sedimentation value and wet gluten content) was investigated. The analysis of variance suggested there were highly significant differences among genotypes (G), investigated years (Y) and locations (L) for sedimentation value and wet gluten content. Apart from individual influence of the factors, their interactions (G x Y, G x L, Y x L, G x Y x L) were also high significant for both investigated traits. In average the highest sedimentation value (40.6 ml) and wet gluten content (31.85 %) established at Backa Topola locality. The highest value of all investigated cultivars and localities established at cultivar Vizija (45.3 ml) in Bačka Topola, while the lowest at Lazarica (31.7 ml) in Sombor. The highest wet gluten content was measured at Bačka Topola locality by cultivar Toplica (38.53 %). In this investigation Bačka Topola locality was favourable for both investigated quality traits