Grafting of leg ulcers with undifferentiated keratinocytes

Brysk, Miriam M.; Raimer, S.; Pupo, Rafael A.; Bell, Trace; Rajaraman, Srinivasan

doi:10.1016/0190-9622(91)70188-8

Cited by 16 publications

(15 citation statements)

References 20 publications

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“…In Table 4, we compared Bioplast ™ CEA with other cultured graft materials. Only two can be directly compared with our study; other studies were not controlled and/or prospectively designed and/or randomized studies 9,39 . The results of our study exceed reports from six cited clinical studies utilizing CEAs, 12 allografts 33–35 and Apligraf ® (Novartis, East Hanover, NJ), a commercially available living skin equivalent 9 .…”

Section: Discussionmentioning

confidence: 77%

“…Clinical observations indicated that upon application, the epidermal sheet was intact, validating the packaging and shipping system. The role of weekly or biweekly debridement cannot be ruled out as a contributing factor in rapid wound healing 39 . In this study, after primary grafting, only those wound areas not viewed by the study director as appreciably healed were fully debrided.…”

Section: Discussionmentioning

confidence: 84%

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Rapid healing of chronic venous stasis leg ulcers treated by the application of a novel serum‐free cultured autologous epidermis

Wille¹,

Burdge

Pitttelkow

2011

Wound Repair Regeneration

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The efficacy and durability of wound closure was examined in a prospective randomized unbalanced clinical trial using the application of a living serum-free cultured epidermal autograft in conjunction with wound-area debridement and a four-layer compression wrap (N=10) compared with wound-area debridement and a four-layer compression wrap in patients with hard-to-heal leg ulcers arising from confirmed venous stasis (N=5). All 15 patients who presented with full-thickness venous ulceration were treated weekly for 8 weeks, with a 12-week final evaluation. The average time to wound closure for the grafted wounds was 4.1 weeks for 80% (8/10) of the cases that closed in 12 weeks compared with 12 weeks for the one closed in the control case. All of the grafted wounds remained closed at 12-month follow-up and one more healed at 30 weeks postenrollment. In the control group, one additional wound healed at 21 weeks postenrollment after the placement of an autograft. No serious adverse events were reported and subjective pain assessment was substantially reduced immediately after graft application. The graft treatment significantly improved outcome and provided durable wound closure. The data suggest that this adaption of this procedure may reduce the management costs of these wound types.

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Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 84%

Rapid healing of chronic venous stasis leg ulcers treated by the application of a novel serum‐free cultured autologous epidermis

Wille¹,

Burdge

Pitttelkow

2011

Wound Repair Regeneration

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“…Phillips et al 5 also achieved a 73% success rate in skin ulcers using allogeneic epidermal sheets of keratinocytes derived from neonatal foreskin. Overall, 114 cases of successful use of allogeneic keratinocyte on wounds have been reported 4–9 . This type of wound coverage using live cells has been defined by the United States Food and Drug Administration as an interactive wound dressing (IWD) 10…”

mentioning

confidence: 99%

Use of a cell‐based interactive wound dressing to enhance healing of excisional wounds in nude mice

Rees

Adamson²,

Lindblad

2001

Wound Repair Regeneration

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The need to have viable, metabolically active cells to heal wounds is well recognized, because there is clear evidence that cellular dysfunction delays healing. This suggests that addition of metabolically active cells to a delayed healing tissue could enhance the healing of the tissue. Therefore, we examined the ability of an interactive wound dressing composed of human keratinocytes or fibroblasts grown on microporous bio-reactor beads and placed into a polyethylene bag to facilitate the delayed healing of wounds in nude mice. A 1 x 1 cm wound was made on the backs of nude mice, and the dressing with or without viable cells was placed on the wound for 8 to 24 days, with dressing changes every other day. Wound area and time to heal measurements were compared after various interventions including freeze-thawing. The data shows that the interactive wound dressing was more effective than the control dressings (p<0.05) and that keratinocytes were more effective than fibroblasts in wound healing (p<0.05). Freezing-thawing of the interactive wound dressings destroyed the activity of the dressing. Studies examining cells using a live/dead viability assay showed that both keratinocytes and fibroblasts were alive after 2 days on the mice. Surprisingly, human fibroblasts appeared to exhibit bridging behavior that is indicative of fibroblast proliferation. We conclude that a simple interactive wound dressing using either keratinocytes or fibroblasts can enhance the healing of wounds in nude mice.

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“…Another technique reported by Brysk et al 67 utilized a polyethylene oxide surgical dressing coated with a thin layer of bovine dermal collagen seeded with keratinocytes. This resulted in complete epithelialization in 7 out of 8 patients with leg ulcers.…”

Section: Cultured Autografts Combined With Cadaveric Dermal Allograftsmentioning

confidence: 99%

Biologic Skin Substitutes

Phillips

1993

The Journal of Dermatologic Surgery and Oncology

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Grafting of leg ulcers with undifferentiated keratinocytes

Cited by 16 publications

References 20 publications

Rapid healing of chronic venous stasis leg ulcers treated by the application of a novel serum‐free cultured autologous epidermis

Rapid healing of chronic venous stasis leg ulcers treated by the application of a novel serum‐free cultured autologous epidermis

Use of a cell‐based interactive wound dressing to enhance healing of excisional wounds in nude mice

Biologic Skin Substitutes

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