Grafting of Detergent-Denatured Skeletal Muscles Provides Effective Conduits for Extension of Regenerating Axons in the Rat Sciatic Nerve.

Mligiliche, Nurru; Kitada, Masaaki; Idé, Chizuka

doi:10.1679/aohc.64.29

Cited by 17 publications

(19 citation statements)

References 21 publications

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“…This simple method of decellularization produces a conduit with architecture similar to that of peripheral nerves. The use of detergent-based methods to facilitate complete removal of cellular material from these scaffolds has shown additional benefits [45,46]. These grafts have been shown to be effective at bridging nerve defects.…”

Section: Discussionmentioning

confidence: 99%

The influence of extracellular matrix derived from skeletal muscle tissue on the proliferation and differentiation of myogenic progenitor cells ex vivo

et al. 2009

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Section: Discussionmentioning

confidence: 99%

The influence of extracellular matrix derived from skeletal muscle tissue on the proliferation and differentiation of myogenic progenitor cells ex vivo

et al. 2009

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“…[108][109] Mligiliche et al were the first to suggest the use of a detergent to denature skeletal muscle fibers to provide effective conduits for regenerating sciatic nerve axons in the rat and rabbit. 110,111 Long-term studies showed that there are length limitations for freeze-thawed muscles grafts to support axonal regeneration. Most studies point to 5 cm, for the largest animal models (sheep femoral nerve), and to less than 2 cm, in the rat sciatic nerve, as the length limit for the successful employment of skeletal muscle grafts.…”

Section: Experimental Studiesmentioning

confidence: 99%

Use of Skeletal Muscle Tissue in Peripheral Nerve Repair: Review of the Literature

Meek¹,

Varejão

Geuna

2004

Tissue Engineering

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The management of peripheral nerve injury continues to be a major clinical challenge. The most widely used technique for bridging defects in peripheral nerves is the use of autologous nerve grafts. This technique, however, necessitates a donor nerve and corresponding deficit. Many alternative techniques have thus been developed. The use of skeletal muscle tissue as graft material for nerve repair is one example. The rationale regarding the use of the skeletal muscle tissue technique is the availability of a longitudinally oriented basal lamina and extracellular matrix components that direct and enhance regenerating nerve fibers. These factors provide superiority over other bridging methods as vein grafts or (non)degradable nerve conduits. The main disadvantages of this technique are the risk that nerve fibers can grow out of the muscle tissue during nerve regeneration, and that a donor site is necessary to harvest the muscle tissue. Despite publications on nerve conduits as an alternative for peripheral nerve repair, autologous nerve grafting is still the standard care for treatment of a nerve gap in the clinical situation; however, the use of the skeletal muscle tissue technique can be added to the surgeon's arsenal of peripheral nerve repair tools, especially for bridging short nerve defects or when traditional nerve autografts cannot be employed. This technique has been investigated both experimentally and clinically and, in this article, an overview of the literature on skeletal muscle grafts for bridging peripheral nerve defects is presented.

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“…Unlike some scaffolds, such as poly‐β‐hydroxybutyrate37 and PLA,38 which may need adding additional stimulating molecules or cells to promote axonal regeneration, acellular muscle itself, can promote axonal regeneration. It has been reported that acellular muscle can promote the axonal regeneration of peripheral nerve 24. The walls of acellular muscle contain laminin, fibronectin, and collagen 23.…”

Section: Discussionmentioning

confidence: 99%

“…The paravertebral muscles were excised, and bundles were longitudinally harvested. The preparation of the chemically extracted acellular muscle was based on a previously described procedure 24. Briefly, the muscle segments were treated with a series of detergent baths (Table I) consisting of Triton X‐100 and sodium dodecyl sulfate (SDS; all reagents from Sigma).…”

Section: Methodsmentioning

confidence: 99%

Chemically extracted acellular muscle: A new potential scaffold for spinal cord injury repair

Zhang

Xue

Liu

et al. 2011

J Biomedical Materials Res

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Extracellular matrix is the gold standard for tissue regeneration. In this study, we directly made the extracellular matrix of the tissue or organ into scaffold for spinal cord injuries, a strategy that is seldomly tried in spinal cord engineering. The aim of this study was to determine if the chemically extracted acellular muscle could be a potential scaffold for spinal cord injury. The chemically extracted acellular muscle was implanted in the lateral hemisected adult rat thoracic spinal cord. Control rats were similarly injured. After 1 and 4 weeks, scaffold integration and biocompatibility, axon sprouting, and myelination were evaluated. The chemically extracted acellular muscle scaffolds were found to be well integrated with the host tissue. Sprouting axons grew into the full length of the scaffold in a strikingly parallel and linear fashion. A few remyelinated axons were also detected in the scaffolds. The tracing results in another six rats showed that labeled fibers entered the chemically treated muscle grafts. Furthermore, there were no apparent quantitative differences in the ED-1 and glial fibrillary acidic protein positive cells between groups. Neuron counting showed more surviving neurons in the acellular muscle treated group than those of the injured only group. Vascularization of the grafts was also confirmed. These findings clearly demonstrated that chemically extracted acellular muscle grafts provided useful biomatrices to enhance axon sprouting in the injured spinal cord.

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Grafting of Detergent-Denatured Skeletal Muscles Provides Effective Conduits for Extension of Regenerating Axons in the Rat Sciatic Nerve.

Cited by 17 publications

References 21 publications

The influence of extracellular matrix derived from skeletal muscle tissue on the proliferation and differentiation of myogenic progenitor cells ex vivo

The influence of extracellular matrix derived from skeletal muscle tissue on the proliferation and differentiation of myogenic progenitor cells ex vivo

Use of Skeletal Muscle Tissue in Peripheral Nerve Repair: Review of the Literature

Chemically extracted acellular muscle: A new potential scaffold for spinal cord injury repair

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