Gracilaria chorda subcritical-water extracts as ameliorant of insulin resistance induced by high-glucose in zebrafish and dexamethasone in L6 myotubes

Thakuri, Laxmi Sen; Park, Chul Min; Park, Jin Woo; Rhyu, Dong Young

doi:10.1016/j.jtcme.2023.07.006

Cited by 2 publications

(2 citation statements)

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“…The results demonstrated that MASW210 modulates the pathophysiology of obesity and metabolic disorders. SWE is a green technique for extracting phytochemicals with high biological activities from extracts and phytochemicals such as phenolic compounds (Romulo, 2020;Thakuri et al, 2023bThakuri et al, , 2023c. Phenolic compounds are vital for defense responses such as antioxidant, anti-inflammatory, and anti-aging activities (Lin et al, 2016;Rahman et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Because zebrafish larvae weigh very light, a pool of 10 larvae was used to measure body weight. Body weight was calculated by removing the water from the pool (Thakuri et al, 2023c). To measure glucose levels, 30 larvae from each group were homogenized using Bullet BlenderTM (Next Advance Lab Products, Palmer, Troy, NY, USA) following the manufacturer's protocol in a capped-microcentrifuge tube with zirconium oxide beads (0.5 mm).…”

Section: Measurement Of Body Weight and Glucose Levelsmentioning

confidence: 99%

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Extracts of abalone intestine regulates fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae

Thakuri,

Park,

Choi

et al. 2024

Fish Aquat Sci

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Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.

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